IBC cell line IOWA-1T (ATCC® CRL-3292™) was purchased from the ATCC cell bank. Breast cancer cell lines MDA-MB-231, T47D, and MCF-7 and healthy mammary fibroblasts Hs578Bst were purchased from the Shanghai Cell Bank. Cells were cultured in 5% CO2 at 37°C in Dulbecco’s modified Eagle’s medium/F-12 HAM (Sigma-Aldrich Chemical Company, St. Louis, MO, USA) supplemented with 50 units/ml penicillin-streptomycin (Sigma-Aldrich Chemical Company, St. Louis, MO, USA) and 10% fetal bovine serum (FBS; Sigma-Aldrich Chemical Company, St. Louis, MO, USA).
Iowa 1t
Manufactured by American Type Culture Collection
The IOWA-1T is a laboratory instrument designed for the cultivation and enumeration of microbial cultures. It provides a controlled environment for the growth and maintenance of a variety of microorganisms, including bacteria, fungi, and cell lines. The core function of the IOWA-1T is to facilitate the propagation and enumeration of microbial samples in a standardized and repeatable manner.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle s medium f 12 ham, by Merck Group (1 mentions)
Hs 578bst, by Shanghai Cell Bank (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Mda mb 231, by Shanghai Cell Bank (1 mentions)
Mcf 7, by Shanghai Cell Bank (1 mentions)
Bt474, by American Type Culture Collection (1 mentions)
Hcc1937, by American Type Culture Collection (1 mentions)
Mda mb 468, by American Type Culture Collection (1 mentions)
Mda mb 453, by American Type Culture Collection (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Skbr3, by American Type Culture Collection (1 mentions)
2 protocols using iowa 1t
1
Culturing Breast Cancer and Healthy Cell Lines
2
Breast Cancer Cell Line Characterization
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Cell lines used in this study include BT20 (catalog number [Cat. No.] HTB-19; ATCC, Manassas, VA, USA), BT474 (Cat. No. HTB-20; ATCC), HCC1937 (Cat. No. CRL-2336; ATCC), K562 (Cat. No. CCL-243; ATCC), MCF-7 (Cat. No. HTB-22; ATCC), MDA-MB-231 (Cat. No. HTB-26; ATCC), MDA-MB-453 (Cat. No. HTB-131; ATCC), MDA-MB-468 (Cat. No. HTB-132; ATCC), Iowa1T (Cat. No. CRL-3292; ATCC), SKBr3 (Cat. No. HTB-30; ATCC). All cell lines were cultured under the conditions recommended by the vendor.
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