For ChIP assays LowCell ChIP kit (C01010071 Diagenode) was used according to manufacturer instructions. Because RbAp48 protein does not bind directly to DNA we used dual crosslinking with 1.5 mM EGS (Invitrogen). For DNA isolation the iPURE v2 (C03010015 Diagenode) kit was used according to manufacturer instructions. Fold enrichment was calculated using the 2−ΔΔCt method.
Ipure v2
Manufactured by Diagenode
The IPURE v2 is a laboratory equipment product from Diagenode. It is designed for nucleic acid extraction and purification. The device performs automated processing of samples to isolate and purify DNA, RNA, or other nucleic acids.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ipure v2
1
Low-cell ChIP Protocol with Dual Crosslinking
2
Low-cell ChIP Protocol with Dual Crosslinking
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For ChIP assays LowCell ChIP kit (C01010071 Diagenode) was used according to manufacturer instructions. Because RbAp48 protein does not bind directly to DNA we used dual crosslinking with 1.5 mM EGS (Invitrogen). For DNA isolation the iPURE v2 (C03010015 Diagenode) kit was used according to manufacturer instructions. Fold enrichment was calculated using the 2−ΔΔCt method.
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