B6 129s6 rag2tm1fwan12 mice

Manufactured by Taconic Biosciences

B6. 129S6-Rag2tm1FwaN12 mice are a genetically modified mouse strain. These mice have a targeted mutation in the Rag2 gene, which results in a severe combined immunodeficiency (SCID) phenotype. This mutation leads to a lack of mature T and B cells, making the mice useful for research involving engraftment of human cells or tissues.

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Lab products found in correlation

B6 sjl ptprca boyaitac, by Taconic Biosciences (1 mentions) C57bl 6n mice, by Inotiv (1 mentions)

2 protocols using b6 129s6 rag2tm1fwan12 mice

Conditional Knockout of Usp9X in Mice

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The loxP-targeted allele of Usp9X was generated by Lexicon using C57BL/6 embryonic stem cells and standard molecular biology techniques. The loxP sites flank exon 31 of Usp9X. Usp9X^fl/fl mice were crossed to C57BL/6-Gt(ROSA)26Sor^{tm16(Cre)Arte} (Taconic), Tg(Vav1-cre)1Cgp (MGI ID: 5527187), or B6.Cg-Tg(CD4.cre)1Cwi N9 (Taconic) deleter strains and backcrossed to a C57BL/6 background for >10 generations. The complete open reading frame of the tdTomato fluorescent protein and a T2A sequence was inserted between the 5′ untranslated region (UTR) and the translation initiation codon present in exon 2 of Usp9X. B6. 129S6-Rag2^tm1FwaN12 mice were purchased from Taconic. Usp9X^fl genotyping primers 5′-GGCAGGTATTCCCACTCAGTAAGTG-3′ and 5′-TGCTGTCTTAAATGCATTTATTAATGGAG-3′ amplified 175-bp (WT) and 260-bp (LoxP) genomic DNA fragments. Usp9X.tdTomato knock-in (KI) genotyping primers 5′-AGGAGGGCAGAGGAAGTC-3′, 5′-CAATGTTGATTTCAGCACCTAAGT-3′, and 5′-TGCCCTGTGTCGAGTATGA-3′ amplified 283-bp (WT) and 324-bp (KI) genomic DNA fragments. All experiments were conducted with the approval of the Genentech Institutional Animal Care and Use Committee.

Naik E., Webster J.D., DeVoss J., Liu J., Suriben R, & Dixit V.M. (2014). Regulation of proximal T cell receptor signaling and tolerance induction by deubiquitinase Usp9X. The Journal of Experimental Medicine, 211(10), 1947-1955.

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Subcutaneous Tumor Model and Vaccination in Mice

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6-8 week old C57BL6/N mice were purchased from Envigo or Taconic Biosciences. 6–8 week old B6.SJL-Ptprc^a/BoyAiTac (known as CD45.1 mice) and B6.129S6-Rag2^tm1Fwa N12 Mice (known as Rag2 KO) were purchased from Taconic Biosciences. Mice were shaved before subcutaneous engraftment of the indicated number of RMA/S cells in 100 uL PBS. Tumor volumes were calculated using caliper measurements and the standard modified ellipsoid formula: tumor volume = (LxW² x 0.52 every 2–3 days. Animals were euthanized when tumor volume exceeded 2000 mm³ or if ulceration was noted. Vaccination of animals was performed by subcutaneous injection of 10 × 10⁶ irradiated (20 Gy) MCA205-ΔTap2 cells expressing libraries of minigenes.

Gejman R.S., Chang A.Y., Jones H.F., DiKun K., Hakimi A.A., Schietinger A, & Scheinberg D.A. (2018). Rejection of immunogenic tumor clones is limited by clonal fraction. eLife, 7, e41090.

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