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Cell counting kit 8 regent

Manufactured by Beyotime
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Sourced in China

The Cell Counting Kit-8 reagent is a colorimetric assay used for the determination of cell viability and cytotoxicity. It utilizes a water-soluble tetrazolium salt that is reduced by cellular dehydrogenases, producing a yellow-colored formazan dye. The amount of the formazan dye, which is directly proportional to the number of living cells, can be measured by absorbance at 450 nm.

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Lab products found in correlation

Microplate spectrophotometer, by Agilent Technologies (1 mentions) Enzyme labeled instrument, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Cell Counting Kit

2 protocols using cell counting kit 8 regent

1

Cell Proliferation Assay Protocol

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In total, 5 × 103 cells were grown in 96-well plates and incubated for 0, 1, 2 and 3 days. At indicated time, 100 μL Cell Counting Kit-8 regent (Beyotime) was added and incubated for another 2 hours. Absorbance was measured at 450 nm using Microplate Spectrophotometer (BioTek Instruments, Inc, Winooski, Vermont).
Yan X., Yu H., Liu Y., Hou J., Yang Q, & Zhao Y. (2019). miR-27a-3p Functions as a Tumor Suppressor and Regulates Non-Small Cell Lung Cancer Cell Proliferation via Targeting HOXB8. Technology in Cancer Research & Treatment, 18, 1533033819861971.
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2

Cell Viability Assay Protocol

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10 μL Cell Counting Kit-8 regent (Beyotime, C0038) was added to 5 × 103 cells per well at 0, 24, 48, and 72 h. Cells were collected, centrifuged at a low speed (JIDI-5R, Guangzhou JiDi Instrument Co., Ltd., China). The optical density was measured with an enzyme-labeled instrument (Thermo Fisher Scientific) at 450 nm.
Deng D., Su Z., Wei B., Zhou J., Yang H, & Liang Z. (2022). Downregulation of miR-146a-5p Promotes Acute Pancreatitis through Activating the TLR9/NLRP3 Signaling Pathway by Targeting TRAF6 In Vitro Rat Model. Computational and Mathematical Methods in Medicine, 2022, 1747470.
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