Anti sox2 mab2018

Using the wild-type ESCs, the Flag-tagged CTBP2 expressing ESCs, the Flag-tagged Zfp217 WT rescued ESCs and the Flag-tagged Zfp217 MUT rescued ESCs, immunoprecipitation and western blot were done as previously described (29 (link)). Anti-Zfp217, anti-Zfp516, anti-Zfp518b, anti-Zbtb8b, anti-Hic2, anti-Wiz, anti-Zscan10, anti-Zscan4f antibodies were made by GenScript (Piscataway, New Jersey). Anti-Oct4 (sc-5279) and anti-Znf217 (sc-55351) were acquired from Santa Cruz Biotechnology (Dallas, TX, USA); anti-Nanog (ab14959), anti-Hdac1 (ab7028), anti-Mi-2b (ab72418), anti-Lsd1 (ab17721), anti-H3 (ab1792), anti-H3K4me1 (ab8895), anti-H3K4me3 (ab8580), and anti-H3K27ac (ab4729) were purchased from Abcam (UK); anti-Ctbp1 (612042), anti-Ctbp2 (612044), and anti-Ezh2 (612667) were acquired from BD Transduction Laboratories (San Jose, CA, USA); anti-Esrrb (H6707) and anti-Sox2 (MAB2018) were obtained from R&D System (Minneapolis, MN, USA); anti-Suz12 (3737S), anti-Bax (2772), anti-Caspase-3 (9662S), and anti-Cleaved Caspase-3 (9661S) were from Cell Signaling Technology (Danvers, Massachusetts); anti-CoREST (07-455) and anti-H3K27me3 (07-449) was obtained from Millipore (Germany); anti-HA tag and anti-Myc tag were acquired from Covance (Princeton, NJ, USA); anti-Flag tag and anti-β-Actin were purchased from (Sigma-Aldrich, St. Louis, MO, USA).