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C57BL/6 MEFs are primary mouse embryonic fibroblasts derived from the C57BL/6 mouse strain. They provide a renewable source of cells for various applications.

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Lab products found in correlation

Dulbecco s modified eagle s medium, by Quality Biological (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Penicillin, by Quality Biological (1 mentions) Streptomycin, by Quality Biological (1 mentions) Fetal bovine serum (fbs), by Quality Biological (1 mentions) Ifn γ, by Thermo Fisher Scientific (1 mentions)

2 protocols using c57bl 6 mefs

1

Cell Culture Protocol for Multiple Cell Lines

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BS-C-1, HeLa, RK-13 and BHK-21 cells were grown in minimum essential medium with Earle’s balanced Salts supplemented with 2 mM L-glutamine, 100 units penicillin and 100 µg streptomycin per ml (Quality Biological, Inc.) and containing 8% fetal bovine serum (FBS)(Sigma-Aldrich). C57BL/6 MEFs (ATCC SCRC-1008) were grown in Dulbecco’s modified eagle’s medium (Quality Biological, Inc.) supplemented with 4 mM L-glutamine, 20% FBS, 100 units penicillin and 100 µg streptomycin per ml.
Liu S.W., Katsafanas G.C., Liu R., Wyatt L.S, & Moss B. (2015). Poxvirus Decapping Enzymes Enhance Virulence by Preventing the Accumulation of dsRNA and the Induction of Innate Antiviral Responses. Cell host & microbe, 17(3), 320-331.
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2

Assessing Parasite Survival in IFN-γ Activated MEFs

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C57BL/6 MEFs (ATCC) were seeded into 24-well trays and were cultured in DMEM in 15% FBS. MEF monolayers were incubated without IFN-γ, or MEF monolayers were stimulated with 200 U/ml IFN-γ (Peprotech) 24 h prior to parasite infection to activate host immunity-related GTPases and cell autonomous killing mechanisms. Triplicate wells of MEFs were infected with 200 or 1,000 tachyzoites, and plaques were allowed to develop for 5 to 7 days. The total number of PFU per well was counted microscopically, and the percentage of PFU survival was calculated as the number of PFU in IFN-γ-stimulated MEFs divided by the number of PFU scored in nonstimulated MEFs (no IFN-γ treatment).
Fox B.A., Rommereim L.M., Guevara R.B., Falla A., Hortua Triana M.A., Sun Y, & Bzik D.J. (2016). The Toxoplasma gondii Rhoptry Kinome Is Essential for Chronic Infection. mBio, 7(3), e00193-16.
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