Mouse anti-GD3 monoclonal antibody (mAb) (R24) was obtained from Dr. L. J. Old (Memorial Sloan-Kettering Cancer Center, New York, USA). Anti-GD2 mAb 220-51 was as described previously.31 (link) Rat anti-mouse CD68 (MCA1957) and rabbit anti-mouse iNOS (PA1-036) were purchased from AbDSerotec (Kidlington, UK). Rabbit anti-Iba1 antibody was from Wako (Cat. No. 019-19741, Osaka, Japan). Alexa568-conjugated anti-rabbit IgG and Alexa488-conjugated anti-rat IgG were purchased from Abcam (Cambridge, UK). Alexa546-conjugated anti-mouse IgG and DAPI were from Thermo Fisher Scientific (Waltham, MA, USA).
Rat anti mouse cd68 mca1957
Manufactured by Bio-Rad
Sourced in United Kingdom
The Rat anti-mouse CD68 (MCA1957) is a laboratory reagent used for the identification and detection of the CD68 antigen, a transmembrane glycoprotein expressed on the surface of macrophages and monocytes. This product can be used in various immunoassay and cell analysis applications.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)
Alexa546 conjugated anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Alexa 568 conjugated anti rabbit igg, by Abcam (1 mentions)
Rabbit anti iba1 antibody, by Fujifilm (1 mentions)
Rat anti mouse gr 1 rb6.8c5 fitc, by BD (1 mentions)
2 protocols using rat anti mouse cd68 mca1957
1
Immunostaining of GD3 and GD2 in Mice
2
Glomerular Immunostaining Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Frozen sections (2 µm) were fixed in ice-cold acetone for 10 min and stained essentially as described previously (Rops et al., 2007b (link)). Directly labeled antibodies included goat anti-mouse C3c and fibrinogen-fluorescein isothiocyanate (FITC) (Nordic, Tilburg, Netherlands), goat anti-rabbit IgG Alexa-488 (Life Technologies, Breda, Netherlands), rat anti-mouse GR-1 (RB6.8C5)-FITC (BD Biosciences, Alphen aan de Rijn, Netherlands), and goat anti-Armenian hamster-Cy3 (Jackson ImmunoResearch Laboratories, West Grove, PA). Unlabeled primary antibodies included rat anti-mouse-CD68 (MCA 1957; Serotec, Oxford, United Kingdom) and hamster anti-agrin (MI91) (Raats et al., 1998 (link)). Sections were fixed with 1% paraformaldehyde–PBS and embedded in VectaShield mounting medium H-1000 (Brunschwig Chemie, Amsterdam, Netherlands). Goat anti-rabbit IgG, goat anti-mouse C3c, fibrinogen, and anti-HS scFv staining intensities were evaluated semi-qualitatively from 0 (no staining) to 10 (100% staining intensity inside the glomeruli) and averaged over 50 glomeruli. All quantitative observations were made by two independent observers on blinded sections. Glomerular influx of granulocytes was determined by counting the number of cells per 50 glomeruli.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!