An Annexin -FITC/PI apoptosis kit (Sigma-Aldrich, St. Louis, MO, USA) was used to detect apoptosis in HeLa and SiHa cells. Cells were cultured as described above and washed with PBS, and then resuspended in 500 μL binding buffer containing 5 μL Annexin -FITC and 5 μL PI for 30 min in dark condition at room temperature. Apoptotic cells were detected using a Beckman Coulter FC 500 ow cytometer and analyzed using the Expo32-ADC software (Beckman Coulter, Miami, FL, USA). All assays were performed in triplicates.
Fc 500 ow cytometer
Manufactured by Beckman Coulter
Sourced in United States
The FC 500 flow cytometer is a versatile and advanced instrument designed for comprehensive cell analysis. It utilizes flow cytometry technology to rapidly analyze and characterize various cell populations within a sample. The FC 500 is capable of detecting and measuring multiple parameters, including cell size, granularity, and fluorescence signals, providing detailed insights into the properties and characteristics of the analyzed cells.
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Lab products found in correlation
4 protocols using fc 500 ow cytometer
1
Annexin-FITC/PI Apoptosis Assay
2
Annexin-FITC/PI Apoptosis Assay
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An Annexin -FITC/PI apoptosis kit (Sigma-Aldrich, St. Louis, MO, USA) was used to detect apoptosis in HeLa and SiHa cells. Cells were cultured as described above and washed with PBS, and then resuspended in 500 μL binding buffer containing 5 μL Annexin -FITC and 5 μL PI for 30 min in dark condition at room temperature. Apoptotic cells were detected using a Beckman Coulter FC 500 ow cytometer and analyzed using the Expo32-ADC software (Beckman Coulter, Miami, FL, USA). All assays were performed in triplicates.
3
Humanized Mouse PBMC Engraftment
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Two weeks after PBMC transplantation, the peripheral blood of mice was collected from the tail vein to detect the content of humanised level. At the completion of the study, mice were euthanised by carbon dioxide inhalation. Spleen and bone marrow were collected immediately after euthanasia. Mouse PBMCs were incubated with uorescently labelled antibodies to determine the levels of human CD45 + CD3 + cells in the blood. Cell acquisition was performed using a FC500 ow cytometer (Beckman Coulter, Miami, FL, USA). Data were analysed using FlowJo software (version 10.7) (TreeStar, San Carlos, CA, USA).
4
Flow Cytometry Characterization of MSCs
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The suspension of live MSCs was characterized on a FC 500 ow cytometer (Beckman Coulter, Miami, FL, USA). MSCs were cultured in 75 cm 2 asks until near con uence. Then, MSCs were harvested using trypsinization with Trypsin 0.05% EDTA (Thermo Fisher Scienti c), washed twice with DPBS 1X, and resuspended in 1x DPBS at 50,000 cells/ml. Anti-CD29, CD73, and CD105 were used as positive markers of MSCs, and CD11 b/c, CD45, CD68, Ia as negative markers.
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