Mats u55r30

Transfected cells were plated onto glass-bottom dishes (Iwaki, Tokyo, Japan) coated with 0.3 mg/mL type-I collagen (Cell matrix I-C; Nitta Gelatin). Two-dimensional time-lapse images were acquired using an inverted microscope (IX71; Olympus) equipped with a single-chip color CCD camera (DP70; Olympus) and an objective lens (UPlanFl 100´/1.30 NA Oil; Olympus). During observation, cells were warmed on a thermoplate set to 37°C (MATS-U55R30; Tokai Hit, Fujinomiya, Japan). Images of rounded cells were acquired every 30 s from anaphase onset using DP Controller software (Olympus).

Time-lapse images were captured with an inverted microscope (IX71; Olympus) equipped with a single-chip color CCD camera (DP70; Olympus) and an objective lens (LCPlanFl 20/0.40 NA; UPlanApo 60/0.90 NA; UPlanFl 100/1.30 NA Oil; Olympus).
During observation, cells were warmed on a thermoplate set to 37°C (MATS-U55R30; Tokai Hit). Images were captured every 5 min and analyzed using Lumina Vision version 2.4.2 software (Mitani Corporation). Images of cells expressing mCherry-NMHC-IIA and EGFP-NMHC-IIB were captured using an inverted microscope (Ti-E; Nikon) equipped with an oil-immersion objective lens (Plan Apo-VC 60/1.40 NA; Nikon). During observation, cells were warmed in an incubation chamber heated to 37°C (INUBG2H-TIZB; Tokai Hit). Images were captured and analyzed using NIS-Elements C software (Nikon).