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Amicon ultra 4 regenerated cellulose 3 kda

Manufactured by Merck Group
Sourced in Italy

The Amicon Ultra-4 regenerated cellulose 3 kDa is a centrifugal filter device designed for the concentration and purification of macromolecules. It has a molecular weight cut-off (MWCO) of 3 kDa, making it suitable for the retention of molecules larger than 3 kDa while allowing the passage of smaller molecules and solutes.

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2 protocols using amicon ultra 4 regenerated cellulose 3 kda

1

Simulated Digestion of Pinto Beans

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Bile salts (mixture of sodium cholate and sodium deoxycholate), porcine α-amylase, pepsin from porcine gastric mucosa, pancreatin from porcine pancreas (4xUSP), angiotensin converting enzyme from rabbit lung, mucin II and III, bovine serum albumin, lysozyme and urea were supplied by Sigma (Milan, Italy). Amicon Ultra-4 regenerated cellulose 3 kDa were supplied by Millipore (Milan, Italy) . Phaseolus vulgaris beans (pinto beans) were purchased from a local market (Reggio Emilia). All electrophoretic, HPLC and MS/MS reagents were from Biorad (Hercules CA, U.S.A.).
All the other reagents were from Carlo Erba (Milan, Italy). The absorbance was read using a Jasco V-550 UV/Vis spectrophotometer (Orlando FL, U.S.A.).
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2

Camel Milk Peptide Isolation Protocol

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Bile salts (mixture of sodium cholate and sodium deoxycholate), porcine α-amylase, pepsin from porcine gastric mucosa, pancreatin from porcine pancreas (4xUSP), ACE from rabbit lung, mucin II and III, bovine serum albumin, 2,4,6-trinitrobenzenesulfonic acid (TNBS), sodium dodecyl sulphate (SDS), dithiothreitol (DTT), lysozyme and urea were supplied by Sigma (Milan, Italy). The tripeptide isoleucine-proline-proline (IPP; 95% purity) was synthesized by DBA (Milan, Italy). Amicon Ultra-4 regenerated cellulose 3 kDa were supplied by Millipore (Milan, Italy). The whole camel milk was obtained from farms at El-Alamin and Sidi-Barani areas around Alexandria (Egypt). All electrophoretic, HPLC and MS reagents were from Bio-Rad (Hercules CA, U.S.A.).
All the other reagents were from Carlo Erba (Milan, Italy).
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