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Annexin 5 propidium iodide double staining test kit

Manufactured by Keygen Biotech
Sourced in China

The Annexin V–propidium iodide (PI) double-staining test kit is a laboratory equipment used for the detection and quantification of apoptotic and necrotic cells in a cell population. The kit utilizes the binding properties of Annexin V to phosphatidylserine, which is exposed on the outer membrane of apoptotic cells, combined with the ability of propidium iodide to stain the nuclei of necrotic cells.

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2 protocols using annexin 5 propidium iodide double staining test kit

1

MG-63 Cells Autophagy Regulation

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MG-63 cells was purchased from ATCC (Manassas, USA). MPPa, Monodansylcadaverine (MDC), Hoechst 33258, 2′,7′-dichlorofluorescin diacetate (DCFH-DA), SP600125, 3-methyladenine (3-MA), Chloroquine (CQ) N-acetyl-l-cysteine (NAC) were purchased from Sigma-Aldrich (St-Louis, MO, USA). Dulbecco modified Eagle medium (DMEM), Fetal bovine serum (FBS) and trypsin were purchased from Gibco (Carlsbad, CA, USA); JC-1 kits was purchased from Beyotime Biotech (Shanghai, China). Cell viability and cytotoxicity test kits (CCK-8) was purchased from Dojindo Molecular Technologies (Kimamoto, Japan). Annexin V–propidium iodide (PI) double-staining test kit was purchased from Keygen Biotech (Nanjing, China). PDT equipment was purchased from Chongqing Jingyu Laser Technology Co. Ltd. (Chongqing, China).
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2

Oxidative Stress and Cell Viability Assays

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MPPa and 2′7′-dichlorofluorescin diacetate (DCFH-DA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). BCA and trypsin were purchased from Beyotime Biotech (Shanghai, China). Cell viability and cytotoxicity test kits [Cell Counting Kit-8 (CCK-8)] were obtained from Dojindo Molecular Technologies (Kumamoto, Japan). Extracellular matrix gel was obtained from BD Biosciences (Franklin Lakes, NJ, USA). Annexin V-propidium iodide (PI) double-staining test kit was purchased from KeyGen Biotech (Nanjing, China). LED equipment was purchased from Chongqing Jingyu Laser Technology Co. Ltd. (Chongqing, China).
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