Glutamax

Manufactured by Omega Scientific

Sourced in United States

GlutaMAX is a laboratory equipment product designed to measure and analyze glutamine levels in biological samples. It provides precise quantification of glutamine concentrations through advanced spectrophotometric techniques.

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Lab products found in correlation

Penicillin streptomycin, by Omega Scientific (2 mentions) Fetal bovine serum (fbs), by Omega Scientific (2 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Glutamax, by Thermo Fisher Scientific (2 mentions) Atplite assay, by PerkinElmer (1 mentions) Prism 5, by GraphPad (1 mentions) Sk mel 5 cells, by American Type Culture Collection (1 mentions) Celltrace violet dye, by Thermo Fisher Scientific (1 mentions) Human ab serum, by Omega Scientific (1 mentions) Kaluza software, by Beckman Coulter (1 mentions) Navios, by Beckman Coulter (1 mentions) Human fibronectin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by GE Healthcare (1 mentions) Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions) Penicillin, by Omega Scientific (1 mentions) Streptomycin, by Omega Scientific (1 mentions) Wnt3a, by R&D Systems (1 mentions)

3 protocols using glutamax

Cytotoxicity Evaluation of Test Compounds

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Human malignant melanoma SKMEL-5 cells were obtained from the American
Type Culture Collection (ATCC HTB-70, Manassas, VA, USA) and were
maintained in 5% CO₂ at 37 °C and cultured in Eagle’s
Minimum Essential Medium (EMEM; Cellgro) plus GlutaMAX supplemented
with 10% fetal bovine serum (FBS; Omega Scientific) and 1% penicillin/streptomycin
(Omega Scientific).
Approximately 30 000 cells were seeded
into individual wells of a 96-well tissue culture plate and incubated
for 24 h. Cells were replenished with fresh medium (0.1 mL/well; 5%
FBS, no antibiotics) and exposed to triplicates of different concentration
solutions (from 0.4 to 100 μM) of test compounds. The analyzed
inhibitors were dissolved in DMSO, reaching a final DMSO concentration
of 1%. After incubation for 24 h at 37 °C and 5% CO₂, cell viability was assessed using ATPlite assay from PerkinElmer
(Waltham, MA). Viability was normalized to control cells which were
treated with the vehicle, DMSO. The reported IC₅₀ values
were calculated by Prism5 (GraphPad).

Barile E., Marconi G.D., De S.K., Baggio C., Gambini L., Salem A.F., Kashyap M.K., Castro J.E., Kipps T.J, & Pellecchia M. (2016). hBfl-1/hNOXA Interaction Studies Provide New Insights on the Role of Bfl-1 in Cancer Cell Resistance and for the Design of Novel Anticancer Agents. ACS Chemical Biology, 12(2), 444-455.

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Allogeneic T Cell Proliferation Assay

Cited 1 time

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The assay was performed as previously described (29 (link)). Briefly, recipient PBMCs were labeled with a CellTrace Violet dye (Invitrogen) and mixed with appropriate donor sBCs at the ratio of 2 sBCs to 1 PBMC. To assess general alloreactivity, a mixture of six sBCs with distinct HLA types was used instead of donor sBC to stimulate the recipient PBMCs. The cells were cultured in RPMI 1640 medium supplemented with GlutaMAX, 10% human AB serum (Omega Scientific), 1% nonessential amino acids, 1% sodium pyruvate, and 1% penicillin/streptomycin (all reagents were from Gibco unless stated otherwise) for 84 to 96 hours before staining with antibodies to CD4, CD8, FOXP3, and HELIOS (table S8) as described above for flow cytometry. The samples were measured on a Navios (Beckman Coulter) flow cytometer, and CellTrace Violet dilution in T_regs (CD4⁺FOXP3⁺HELIOS⁺), T_conv (CD4⁺, not T_reg gate), and CD8⁺ T cells were analyzed using Kaluza software (Beckman Coulter).

Tang Q., Leung J., Peng Y., Sanchez-Fueyo A., Lozano J.J., Lam A., Lee K., Greenland J.R., Hellerstein M., Fitch M., Li K.W., Esensten J.H., Putnam A.L., Lares A., Nguyen V., Liu W., Bridges N.D., Odim J., Demetris A.J., Levitsky J., Taner T, & Feng S. (2022). Selective decrease of donor-reactive Tregs after liver transplantation limits Treg therapy for promoting allograft tolerance in humans. Science translational medicine, 14(669), eabo2628.

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Routine culture of mouse embryonic stem cells

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R1 mESCs were purchased from ATCC and cultured at 37°C and 5% CO₂. Routine culture of mESCs was carried out in media containing DMEM with GlutaMAX (Thermo Fisher, Waltham, MA), 15% fetal bovine serum (GE Healthcare, Chicago, IL), 1 mM sodium pyruvate, MEM nonessential amino acids, 50 μM β-mercaptoethanol, 100 U/ml penicillin, 100 μg/ml streptomycin (all from Thermo Fisher), and 1000 U/ml LIF (Millipore Sigma, Burlington, MA) in tissue culture plates coated with 0.1% gelatin. For serum-free culture, cells were maintained in one volume DMEM/F12 with GlutaMAX combined with one volume Neurobasal media, with 0.5% N2 Supplement, 1% B27 Supplement, 0.033% bovine serum albumin (BSA), 50 μM β-mercaptoethanol, 100 U/ml penicillin, and 100 μg/ml streptomycin (all from Thermo Fisher) in tissue culture plates coated with 2.5 μg/cm² human fibronectin (Thermo Fisher). HEK293T cells and L cells were cultured in DMEM with GlutaMAX supplemented with 10% fetal bovine serum (FBS; Omega Scientific, Tarzana, CA), 100 U/ml penicillin, and 100 μg/ml streptomycin. Carrier-free recombinant mouse Wnt3a was purchased from R&D, reconstituted in 0.1% BSA/phosphate buffered saline (PBS), and added to culture at specified concentrations.

Rim E.Y., Kinney L.K, & Nusse R. (2020). β-catenin-mediated Wnt signal transduction proceeds through an endocytosis-independent mechanism. Molecular Biology of the Cell, 31(13), 1425-1436.

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