Golden gate single reflection diamond atr unit

IR spectra (128 scans, 4 cm⁻¹ spectral resolution) of the CD-complexes were recorded in attenuated total reflection (ATR) mode using a dynamically aligned Varian 2000 FTIR spectrometer (Varian Inc., Palo Alto, CA, USA) equipped with a liquid nitrogen cooled broadband MCT detector and fitted with a ‘Golden Gate’ single reflection diamond ATR unit (Specac Ltd., Orpington, UK). 5 μL of sample solution was spread on the top of the ATR crystal and a thin dry film was obtained by evaporation of the aqueous solvent under ambient conditions. All spectra were ATR corrected. For spectral manipulations the GRAMS/AI (7.02) software package was used.

Infrared spectroscopic investigation was performed using the attenuated total reflection (ATR) technique. A Varian Scimitar 2000 FT-IR spectrometer (Varian Inc., Palo Alto, CA, USA) equipped with an MCT (mercury–cadmium–telluride) detector was used and fitted with a ‘Golden Gate’ single reflection diamond ATR unit (Specac Ltd., Cray Ave, Orpington, UK). During the measurements, the solid samples, without any sample preparation, were pressed with a constant 70 cNm pressure on the top of the diamond ATR crystal by a sapphire anvil. All spectra were collected with a nominal resolution of 4 cm⁻¹ by the co-addition of 128 individual spectra. Before spectral evaluation, ATR correction was performed. Raman spectra were recorded with a Bio-Rad (Digilab) dedicated FT-Raman spectrometer equipped with a Spectra-Physics Nd-YAG-laser (1064 nm) and liquid-N₂ cooled Ge detector. The power of the excitation laser was about 500 mW at the samples. All spectra were collected using a backscattered geometry with a nominal resolution of 4 cm⁻¹ and by the co-addition of 128 individual scans.