Sgc7901 ddp

Normal gastric mucosal cell line GES-1 and parental GC cell lines BGC823 and SGC7901, as well as corresponding DDP-resistant strains (BGC823/DDP and SGC7901/DDP) were all purchased from the American Type Culture Collection (ATCC; Rockville, MD, USA). All the cells were cultured in PRMI-1640 medium (Gibco, USA) containing 10% bovine fetal serum (Gibco, USA) 37 °C with 5% CO₂.

The human gastric mucosal cell GES-1, human GC cell line SGC-7901, and chemoresistant GC cell line SGC-7901/DDP were obtained from the American Type Culture Collection. These cells were cultured in RPMI-1640 medium (Hyclone, USA) with 10% fetal bovine serum (Hyclone, USA) at 37°C in 5% CO₂ atmosphere. The 1 μg/mL of DDP (J&K, China) was added to the corresponding cell group. The plasmids of pGL3-ABCC2-wt, pGL3-ABCC2-mut, pcDNA3.1-ABCC2, shRNA-ABCC2, miR-424-3p mimics, miR-424-3p inhibitor, and control vectors were obtained from GeneCopoeia (Guangzhou, China), Obio technology (Shanghai, China), and some of them were constructed by ourselves. The plasmids were transfected into cells by using transfection reagents (Roche, Switzerland).