Rosettesep human ilc2 enrichment kit

Manufactured by STEMCELL

Sourced in United Kingdom

The RosetteSepTM Human ILC2 Enrichment Kit is a cell separation product designed to isolate type 2 innate lymphoid cells (ILC2s) from human peripheral blood mononuclear cells (PBMCs) or other cell suspensions. The kit utilizes a negative selection approach to enrich for ILC2s by targeting and removing unwanted cell types.

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Lab products found in correlation

Interleukin 2 (il 2), by Thermo Fisher Scientific (2 mentions) Il 33, by Thermo Fisher Scientific (2 mentions) Interleukin 7 (il 7), by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

RosetteSep (157 citations) RosetteSep kit (26 citations)

2 protocols using rosettesep human ilc2 enrichment kit

Isolation and Culture of ILCs

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ILCs were enriched from whole blood (adult mild asthmatics and healthy controls) using RosetteSep^TM Human ILC2 Enrichment Kit (Stemcell Technologies, Cambridge, UK) and sorted by flow cytometry using CD45⁺ lineage^neg (CD1a, CD3, CD4, CD5, CD8, CD11c, CD14, CD16, CD19, CD20, CD34, FcεRI and CD123) (BioLegend), CD161⁺, CD127⁺, CRTH2⁺ and C-Kit^var. In order to fully differentiate between lineage negative and positive cells after RosetteSep^TM antibody cocktail staining, an expanded lineage panel was used. ILCs were cultured in IL-2, IL-7 (5 ng·mL⁻¹) and IL-33 (10 ng·mL⁻¹) (eBioscience). ILC cultures were stimulated as described for PBMC cultures.

Nagakumar P., Puttur F., Gregory L.G., Denney L., Fleming L., Bush A., Lloyd C.M, & Saglani S. (2019). Pulmonary type-2 innate lymphoid cells in paediatric severe asthma: phenotype and response to steroids. The European Respiratory Journal, 54(2), 1801809.

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Isolation and Culture of Human ILC2

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ILC2 were enriched from whole blood using RosetteSepTM Human ILC2
Enrichment Kit (STEMCELL Technologies) and further sorted by FACs using
CD45⁺ Lineage^neg (CD1a, CD3, CD4, CD5, CD8, CD11c,
CD14, CD16, CD19, CD20, CD34, FcγRI and CD123) (Biolegend),
CD161⁺, CD127⁺, CRTH2⁺ and
C-Kit^var (Biolegend). ILC2 were bulk cultured in IL-2, IL-7
(5ng/ml) and IL-33 (10ng/ml) (eBioscience) and cytokines and medium were
replenished every 3-4 days. For investigations involving humans ILC2 isolation,
an informed consent was obtained and the nature and possible consequences of the
studies were explained to all donors.

Puttur F., Denney L., Gregory L.G., Vuononvirta J., Oliver R., Entwistle L.J., Walker S.A., Headley M.B., McGhee E.J., Pease J.E., Krummel M.F., Carlin L.M, & Lloyd C.M. (2019). Pulmonary environmental cues drive group 2 innate lymphoid cell dynamics in mice and humans. Science immunology, 4(36), eaav7638.

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