Anti α catenin

Proteins were lysed by radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime Institute of Biotechnology, Shanghai, P.R. China), separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel and then transferred into polyvinylidene difluoride (PVDF) membranes (Bio-Rad Laboratories, Inc., Hercules, CA, USA). After blocking with 5% skim milk at room temperature for 1 h, the membranes were incubated with primary antibodies overnight at 4°C. After being incubated with corresponding secondary antibodies (1:5,000; Cell Signaling Technology, Beverly, MA, USA) at room temperature for 1 h, the enhanced chemiluminescence (ECL) system (Bio-Rad Laboratories) was used for detection. The primary antibodies used in our study were anti-octamer-binding transcription factor 4 (OCT4), anti-sex-determining region Y box 2 (SOX2), anti-NANOG, anti-epithelial (E)-cadherin, and anti-vimentin (Cell Signaling Technology); anti-α-catenin, anti-β-catenin, anti-fibronectin, anti-c-myc, and anti-survivin (Abcam, Cambridge, UK); and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Sigma-Aldrich).