Aperio cs2 pathology slide scanner

Tissue sections were imaged using an LSM880 confocal microscope (Zeiss) equipped with a motorized stage using 10× (0.3 NA) or 20× (0.8 NA) objective lenses with identical settings across all experimental conditions. The lasers used were as follows: argon 458, 488 and 514; diode 405; and DPSS 561–10. The DPSS 561–10 laser intensity was increased during imaging of the control perfusate samples for the intravascular haemoglobin fluorescence study to obtain a background signal that was comparable to the other groups. Images were acquired at either 1,024 × 1,024 or 2,048 × 2,048 pixel resolution. The images are either representative confocal tile scans, high-magnification maximum intensity z-stack projections (approximately 7–9 μm stacks; ~1 μm per z-step) or high-magnification confocal images. Alternatively, histological images were acquired using the Aperio CS2 Pathology Slide Scanner (Leica) as described above. Image adjustments were uniformly applied to all of the experimental conditions in Zeiss Zen. Digitized images were assembled in Zeiss Zen, ImageScope and Adobe Illustrator.

Tissue sections were imaged utilizing an LSM880 confocal microscope (Zeiss; Jena, Germany) equipped with a motorized stage using 10x (0.3 NA) or 20x (0.8 NA) objective lenses with identical settings across all experimental conditions. Lasers utilized: Argon 458, 488, and 514; Diode 405; and DPSS 561–10. The DPSS 561–10 laser intensity was increased during imaging of the control perfusate samples for the intravascular hemoglobin fluorescence study in order to obtain a background signal comparable to other groups. Images were acquired at either 1024 × 1024 or 2048 × 2048 pixel resolution. Images are either representative confocal tile scans, high-magnification maximum intensity Z-stack projections (approximately 7–9 micron stacks; ~1 micron per z-step), or high-magnification confocal images. Alternatively, histological images were acquired with an Aperio CS2 Pathology Slide Scanner (Leica; Wetzlar, Germany) as described above. Image adjustments were uniformly applied to all experimental conditions in Zeiss Zen. Digitized images were assembled in Zeiss Zen, ImageScope, and Adobe Illustrator.