Immunohistochemical (IHC) staining was performed as described below. The tumor specimens were fixed with 10% paraformaldehyde and embedded in paraffin. The sections were incubated with IL-10 primary antibody (1:100, Abcam, USA), followed by the appropriate secondary antibody, and visualized with diaminobenzidine (DAB). Finally, the slides were counterstained with hematoxylin. For the evaluation of the IHC results, we defined low expression (immunohistochemistry score 0 or 1) and high expression (immunohistochemistry score 2).
Il 10 primary antibody
Manufactured by Abcam
Sourced in United States
The IL-10 primary antibody is a research-use-only product that specifically recognizes the interleukin-10 protein. It can be used in various immunoassay techniques to detect and measure IL-10 levels in biological samples.
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Lab products found in correlation
2 protocols using il 10 primary antibody
1
Immunohistochemical Staining of IL-10
2
Immunofluorescence Staining of Liver Macrophages
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Liver macrophages were isolated for immunofluorescence staining of IL-1 and IL-10 by seeding them into 6-well plates and incubating with different treatments. After incubation, the cells were fixed with cold methanol: acetone (1∶1) for 10 min on ice. After washing the cells with PBS (pH 7.2) and blocking in solution containing 1% immunohistochemical-grade bovine serum albumin (BSA; Vector Laboratories, Burlingame, CA, U.S.A) the cells were incubated overnight with an IL-1 primary antibody (Santa Cruz biotechnology, Dallas, USA) and IL-10 primary antibody (Abcam, Cambridge, USA) at 4°C. After three washes with blocking solution, the sections were incubated for 30 min at room temperature with secondary antibodies (Antgene, Wuhan, China) and viewed by confocal laser microscopy.
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