Vac stova

The animals were randomly divided into four groups (n = 15), and stimulated by chronic ovalbumin (OVA) to establish an asthmatic model [4] (link). In brief, on day 0 and 7, the mice were sensitized by intravenous injection of 0.2 mL saline solution containing 20 μg OVA (vacstova, Invivogen, Toulouse, France) and 2 mg aluminum hydroxide (HY-B1521, MedChem Express, Shanghai, China). The mice were exposed to 2% OVA solution for 30 min by ultrasonic nebulizer from day 14 on three times a week for 6 weeks (Figure 1(a)). Mice in the control group were only sensitized and treated by saline solution. From day 14 on, the mice in control and asthmatic (OVA) groups were orally administrated with saline solution, while those in Bulleyaconitine A (BLA) group were intraperitioneally given BLA (0.48 mg/kg). In tiotropium bromide (Tio) group, the mice received intranasal drip of 0.1 mm Tio (S61229, Yuanye) 30 min before the OVA stimulation and were considered as a positive drug group. After the completion of the program, the animals were anesthesed by intraperitoneal injection of overdose of sodium pentobarbital (R20830, Yuanye), and sacrificed by cervical dislocation method. Finally, the blood and lung tissues of the animals were obtained for subsequent analysis.