At the end of the experiment, the thoracic aorta (from the arch to the diaphragm) was harvested, cut in half, and either fixed in buffered formalin or snap frozen. Aorta rings were embedded in paraffin and sections were cut at 4 μm and prepared for hematoxylin and eosin (HE) staining. Immunohistochemical detection of proliferating cell nuclear antigen (PCNA) was performed using an anti-PCNA primary antibody (1 : 500) and a horseradish peroxidase-conjugated goat anti-rat Ig secondary antibody (1 : 100) (Invitrogen, Basel, Switzerland).
Horseradish peroxidase conjugated goat anti rat ig secondary antibody
Manufactured by Thermo Fisher Scientific
Sourced in Switzerland
Horseradish peroxidase-conjugated goat anti-rat Ig secondary antibody is a laboratory reagent used in immunoassays and other immunological techniques. It consists of a goat-derived antibody that specifically binds to rat immunoglobulins, conjugated with the enzyme horseradish peroxidase. This reagent can be used to detect and quantify the presence of rat antibodies in samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using horseradish peroxidase conjugated goat anti rat ig secondary antibody
1
Thoracic Aorta Histological Assessment
2
Renal Function and Histology Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The GFR was assessed by the inulin clearance test as previously described, in anesthetized mice. 35 Renal histology, immunohistochemistry, and crystal anisotropism Kidneys were embedded in paraffin and 4-mm sections were prepared for hematoxylin and eosin staining. Immunohistochemical detection of the macrophage marker F4/80 and lymphocyte marker CD3 was performed using respectively: rat anti-F4/80 primary antibody (1:800) and a horseradish peroxidase-conjugated goat antirat Ig secondary antibody (1:100) (Invitrogen, Basel, Switzerland); rabbit anti-human CD3 primary antibody (1:100, DAKO, Baar, Switzerland) and the DAKO EnVision þ Rabbit HRP ready-to-use kit (cat. K4002). Cryosections of kidney were obtained from absolute ethanol-fixed kidneys to assess anisotropism of uric acid crystals under polarized light.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!