Goat anti mouse secondary antibodies

Antibodies against AMPKα1/2, AMPKα2, p‐AMPKα1/2(Thr¹⁸³/Thr¹⁷²), phospho‐ACC (Ser⁷⁹), ACC, and 14‐3‐3 were obtained from Abcam (San Francisco, CA, USA). TBC1D1 and p‐TBC1D1 (Ser²³⁷) were purchased from Merck Millipore (Darmstadt, Germany). Antibodies against HDAC5 and p38 MAPK were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against β‐actin were purchased from Sigma‐Aldrich (St. Louis, MO, USA). Antibodies against phospho‐p38 (Thr¹⁸⁰/Tyr¹⁸²), α‐tubulin, GLUT4, and lamin B were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Phospho‐HDAC5 (Thr⁴⁹⁸) antibodies were from Thermo Fisher Scientific (Rockford, IL, USA). Horseradish peroxidase (HRP)‐conjugated goat anti‐rabbit IgG and goat anti‐mouse secondary antibodies were purchased from Enzo Life Sciences (Farmingdale, NY, USA). Metrnl was obtained from Cusabio (Wuhan, Hubei, China), and 1, 2‐bis (o‐aminophenoxy) ethane‐N, N, N′, N′‐tetraacetic acid (BAPTA)‐AM was purchased from Abcam. Compound C and STO‐609 were obtained from Calbiochem (San Diego, CA, USA). Protein A‐agarose beads were obtained from GE Healthcare (Piscataway, NJ, USA). The fluorescent Ca²⁺ indicator Fluo‐3 AM and Hoechst 33342 were obtained from Invitrogen (Leiden, the Netherlands).

Antibodies against protein phosphatase 2 A (PP2A) were purchased from Merck Millipore (Darmstadt, Germany). Antibodies against phospho-PP2A (Tyr³⁰⁷), phospho-protein kinase C alpha (PKCα) (Thr⁶³⁸), PKCα, phospho-ERM (Thr⁵⁶⁷), and Src were purchased from Abcam (MA, USA). Antibodies against phospho-Src (Tyr⁴¹⁶) and ERM were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against β-actin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-vimentin antibody and anti-HA tag antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG and goat anti-mouse secondary antibodies were purchased from Enzo Life Sciences (Farmingdale, NY, USA). Resistin was obtained from Phoenix Pharmaceuticals (Belmont, CA). 1,2-Bis(o-aminophenoxy) ethane-N,N,N′,N′-tetraacetic acid (BAPTA)-AM was purchased from Abcam (MA, USA). Gö6976 and PP2 were obtained from Calbiochem (San Diego, CA, USA). Okadaic acid was purchased from Sigma-Aldrich. Protein A-agarose beads were obtained from GE Healthcare (Piscataway, NJ, USA). Fluo-3 AM was obtained from Invitrogen (Leiden, Netherlands). Ezrin-EGFP plasmid was kindly provided to us by Dr. Y. H. Moon (Korea University, Seoul, Korea), and the PKCα-DS-Red plasmid was kindly provided to us by Dr. Kwang-Youl Lee (Chonnam University, Kwangju, Korea).