Serum fasting blood glucose (FBG), total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-c), high-density lipoprotein cholesterol (HDL-c), free fatty acids (FFAs), lipoprotein(a) [Lp(a)], uric acid (UA), and high-sensitivity C reactive protein (hsCRP) were measured by routine automated laboratory methods. Serum insulin, fibroblast growth factor 21 (FGF21), glucagon-like peptide 1 (GLP-1), peptide YY (PYY), and leptin levels were measured by ELISA kits (CEA448Ra, CEC918Ra, CEA804Mi, CEB067Ra, and SEA084Ra, Wuhan USCN Business Co., Ltd., Wuhan, China) following the instructions of the manufacturers. The coefficients of variation for intraassay of insulin, FGF21, GLP1, PYY, and leptin were 2.9, 3.1, 2.0, 1.9, and 3.0%, respectively. Homeostasis model assessment of insulin resistance (HOMA-IR) was calculated according to the following formula: fasting serum insulin (pmol/L) × FBG (mmol/L)/135 (Yan et al., 2017 (link)).
Cea448ra
Manufactured by USCN
Sourced in China
The CEA448Ra is a laboratory equipment product. It is a quantitative enzyme-linked immunosorbent assay (ELISA) kit designed to measure the concentration of the target analyte in a sample.
Automatically generated - may contain errors
5 protocols using cea448ra
1
Cardiometabolic Biomarker Assessment
2
Cytokine Profiling in Animal Model
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Cardiac blood was collected from animals at week 7 for cytokine analyses. Serum levels of C-peptide Cloudclone (CEA447Ra, Uscn Life Science Inc. Wuhan, China), insulin (CEA448Ra, Uscn Life Science Inc. Wuhan, China), Th1-related IFN-γ (SEA049Ra, Uscn Life Science Inc. Wuhan, China), Th17-related IL-17 (SEA063Ra, Uscn Life Science Inc. Wuhan, China) as pro-inflammatory, and Th2-related IL-4 (SEA063Ra, Uscn Life Science Inc. Wuhan, China) and Treg-related IL-10 (SEA056Ra, Uscn Life Science Inc. Wuhan, China) cytokines as anti-inflammatory were evaluated by ELISA analysis. The manufacturer’s instructions were followed during the analyses, and the measurements were made with a 450-nm microplate reader (Allsheng AMR-100 Microplate Reader AS-16050-00). With the measured absorbance values, the concentrations of the samples were calculated as pg/ml.
3
Oral Glucose Tolerance Test in Rats
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For OGTT, rats were fasted for 12 h and followed by an oral administration of glucose load (2 g/kg of body weight). The blood glucose (BG) levels were determined by a blood glucose test strip (Sinocare, Changsha, China) in tail blood at 0, 30, 60 and 120 min after glucose treatment. The area under the curve (AUC) was calculated using the following equation: AUC = 0.5 × (BG0 + BG30)/2 + 0.5 × (BG30 + BG60)/2 + 1×(BG60 + BG120)/2 according to the previous report [20 ]. The SD rats were fasted overnight and sacrificed at the 16th week. The fast blood glucose level was measured by glucometer (Sinocare, Changsha, China). The levels of insulin in serum were determined by ELISA kit (CEA448Ra, USCN Life Science) according to the manufactures’ protocol. The HOMA-IR was calculated as follows: fasting blood glucose (mmol/L) × fasting insulin (mIU/L)/22.5.
4
Insulin Secretion Assay in Glucose-Starved INS-1 Cells
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INS-1 cells were starved in glucose–free RPMI 1640 for 2 h after the pretreatments, and then washed twice in HEPES-balanced Krebs–Ringer bicarbonate (KRB) buffer containing 2 g/L bovine serum albumin ((KRB/BSA) before the exposure to 8.3 mM glucose (in KRB buffer) for 1 h [22 (link)]. The levels of insulin secretion were measured by ELISA according to the manufacture’s instruction (CEA448Ra, USCN Life Science, Wuhan, China).
5
Enzyme-linked Immunosorbent Assay for CEA
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To achieve this measurement, we used a commercially available Enzyme-linked Immunosorbent Assay kit, CEA448Ra, following the instructions of the supplier (USCN Life sciences Inc., Wuhan, Hubei, China).
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