Electrochemiluminescence ecl reagent

4,5-Dimethylthiazol-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) was bought from MP Biomedicals (California, USA). Annexin V/propidium iodide (PI) apoptosis kit was purchased from MultiSciences Biotech Co., Ltd (Hangzhou, China). BCA protein assay kit was bought from Thermo Fisher Scientific, Inc (ML, USA). Rabbit anti-human PIK3CA, EGFR, phospho-EGFR (p-EGFR), AKT, p-AKT, ERK, and p-ERK monoclonal antibodies (mAb) and horseradish peroxidase (HRP) conjugated anti-rabbit antibody were purchased from Cell Signaling Technology, Inc (MA, USA). Electro-Chemi-Luminescence (ECL) reagent was purchased from Millipore Corporation (MA, USA).

Primers, plasmid extraction kit and gel recovery kit were purchased from Shanghai Bio-engineering (Shanghai, China). Lipofectamine 2000 reagent was purchased from Invitrogen (Carlsbad, CA, USA). Fetal bovine serum (FBS) was purchased from Hyclone (Logan, USA). Electrochemiluminescence (ECL) reagent was purchased from Millipore (Billerica, MA, USA). RIPA lysis buffer, protease inhibitor and BCA protein assay kit were purchased from Beyotime Institute of Biotechnology (Jiangsu, China). Stathmin primary antibody was purchased from Abcam (Abcam, UK). Goat anti-rabbit/mouse secondary antibody was purchased from Jackson.

Ginsenoside Rb1 (purity > 99.5%) was purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). SSD cream (1%) was used as a positive control.Anti-FGF-2, anti-PDGFR-β, anti-PDGF-BB and anti-β-actin antibodies were purchased from Abcam (Shanghai, China). A BCA protein quantification kit was purchased from BiyunTian (Shanghai, China). Electrochemiluminescence (ECL) reagent was purchased from Millipore (Billerica, MA, USA). A total RNA extraction kit was purchased from Tiangen (Beijing, China). A RevertAid First Strand cDNA Synthesis Kit was purchased from Thermo Fisher Scientific (Waltham, MA, USA). RT-PCR Master Mix was purchased from Vazyme (Nanjing, China).The various other chemicals used were of analytical grade and commercial origin.