Primary antibody against actin

RPMI-1640 medium, fetal bovine serum, penicillin (FBS), streptomycin were products of GIBCO BRL (Grand Island, NY) and materials for culturing H. pylori were sheep blood agar, Gaspak and anaerobic jars (BD Biosciences, Sparks, MD). Primary antibody against actin was purchased from Sigma-Aldrich Co. (St. Louis, MO), antibodies for lamin B from Santa Cruz Biotechnology (Santa Cruz, CA), other antibodies for p-STAT3^Tyr705, total STAT3 from Cell Signaling Technology (Beverly, MA), horseradish peroxidase (HRP)-conjugated secondary antibody from Pierce Biotechnology (Rockford, IL). DL-dithiothreitol (DTT), TRIzol, 4',6-diamidino-2-phenylindole (DAPI) from Invitrogen (Carlsbad, CA), and polyvinylidene difluoride (PVDF) membranes were supplied from Gelman laboratory (Ann Arbor, MI). The ECL chemiluminescent detection kit was purchased from LPS solution (Daejon, South Korea) and protein assay dye (Bradford) reagent was supplied by Bio-Rad Laboratories (Hercules, CA), bicinchonic acid (BCA) protein assay reagent was obtained from Pierce Biotechnology.

Galangin (3,5,7-trihydroxyflavone) and primary antibodies against ERK, phospho-ERK, GCLC, and GSS were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and primary antibody against actin were purchased from Sigma-Aldrich Chemical Company (St. Louis, MO, USA). Buthioninesulfoximine (BSO) was purchased from Cayman Chemical (Ann Arbor, MI, USA). Primary antibodies against TATA-binding protein (TBP) and phospho-Nrf2 were purchased from Abcam (Cambridge, MA, USA). Primary antibodies against Nrf2, AKT, and phospho-AKT were purchased from Cell Signaling Technology (Beverly, MA, USA). Cell Tracker^TM Blue CMAC was purchased from Molecular Probes (Eugene, OR, USA). All other chemicals and reagents were of analytical grade.

The primary antibody against MMP-1 was purchased from Epitomics (Burlingame, CA, USA), and the primary antibody against actin was obtained from Sigma-Aldrich (St. Louis, MO, USA). The primary antibodies against c-Jun and phosphorylated (phospho)-c-Jun were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). All other chemicals and reagents were of analytical grade, unless otherwise stated.