Cd207 pe

Manufactured by Beckman Coulter

Sourced in United States

The CD207 PE is a fluorescently-labeled antibody used in flow cytometry applications. It binds to the CD207 (Langerin) antigen expressed on certain immune cells.

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Lab products found in correlation

Facsdiva software, by BD (1 mentions) Cd45 percp, by BD (1 mentions) Live dead stain, by Thermo Fisher Scientific (1 mentions) Facsaria instrument, by BD (1 mentions) Cd1a fitc, by BD (1 mentions) Cd45 v500, by BD (1 mentions) Cd19 fitc, by Thermo Fisher Scientific (1 mentions) Cd3 percp, by BD (1 mentions) Recombinant human tnf, by R&D Systems (1 mentions) Cd3 af700, by BD (1 mentions) Cd1a apc, by BD (1 mentions) Tripalmitoylated lipopeptide pam3csk4, by InvivoGen (1 mentions) Cd20 fitc, by Thermo Fisher Scientific (1 mentions) Dc sign fitc, by R&D Systems (1 mentions)

Close spelling variants of this product

CD207 (2 citations)

2 protocols using cd207 pe

FACS Purification of Primary LCH Samples

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The following antibodies were used for FACS purification of primary LCH samples: CD45-PerCP (345809, BD Biosciences), CD1A-FITC (555806, BD Biosciences), CD207 PE (IM3577, Beckman Coulter), CD14 Alexa Fluor 700 (A7-212-T100, Exbio). To selected antibodies for the analysis of LCH subsets, we cross-checked marker gene lists for commercially available, high-quality antibodies. The following antibodies were used for FACS purification of LCH subsets: CD207 APC-Vio770 (130-112-371, Miltenyi Biotec), CD1A PE-Vio 770 (130-112-872, Miltenyi Biotec), CD45 eFluor 506 (69-0459-42, eBioscience), CD168 FITC (bs-4736R-FITC, Bioss), CD208 PE (130-104-392, Miltenyi Biotec), CD298 PerCP-Vio770 (130-101-294, Miltenyi Biotec), CD300A Alexa 647 (566342, BD Biosciences), and CD370 VioBlue (130-097-406, Miltenyi Biotec). LIVE/DEAD stain (Invitrogen) was used for LIVE/DEAD staining according to manufacturer’s description. Cell sorting was performed on a FACSAria instrument (BD Biosciences). The FACSDiva software (BD Biosciences) was used for data analysis.

Halbritter F., Farlik M., Schwentner R., Jug G., Fortelny N., Schnöller T., Pisa H., Schuster L.C., Reinprecht A., Czech T., Gojo J., Holter W., Minkov M., Bauer W., Simonitsch-Klupp I., Bock C, & Hutter C. (2019). Epigenomics and Single-cell Sequencing Define a Developmental Hierarchy in Langerhans Cell Histiocytosis. Cancer discovery, 9(10), 1406-1421.

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Multicolor Flow Cytometry Assay for Viral Infection

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The following reagents were used: tripalmitoylated‐lipopeptide Pam3CSK4 (5 μg/mL) (Invivogen, San Diego, CA, USA), recombinant human TNF (1 μg/mL) (R&D Systems, Minneapolis, MN, USA). The following antibodies were used (all anti‐human): CD3‐AF700, CD3‐PerCP (BD Biosciences, San Jose, CA, USA), CD19‐FITC, CD20‐FITC, CD56‐FITC, (Thermo Fisher Scientific Waltham, MA, USA), CD45‐V500, (BD Horizon, USA), CD1a‐APC (BD Biosciences, San Jose, CA, USA), CD207‐PE (Beckman Coulter, Indianapolis, IN, USA), DC‐SIGN‐FITC (R&D Systems) and anti‐HIV‐1 p24, KC57‐RD1‐PE, (Beckman Coulter). The following reagents were obtained through the NIH AIDS Reagent Program, NIAID: Zidovudine (10 μmol/L), Raltegravir (100 nmol/L) and Indinavir (1 μmol/L).
The following plasmids were provided by Dr. Takaji Wakita at Tokyo Metropolitan Institute of Neuroscience: Genotype 2a HCV genomic RNA clone pJFH1 (APP1025) 27. pJFH1‐AM120‐Rluc was provided by Dr. Curt Hagedorn at University of Utah, Salt Lake City, USA. pNL4.3.Luc_RΔenv provided by Dr. N.R. Landau 28, pHCV_H77_E1_E2(AF009606) Dr. Joe Grove (Addgene) 29.

Nijmeijer B.M., Sarrami‐Forooshani R., Steba G.S., Schreurs R.R., Koekkoek S.M., Molenkamp R., Schinkel J., Reiss P., Siegenbeek van Heukelom M.L., van der Valk M., Ribeiro C.M, & Geijtenbeek T.B. (2019). HIV‐1 exposure and immune activation enhance sexual transmission of Hepatitis C virus by primary Langerhans cells. Journal of the International AIDS Society, 22(3), e25268.

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