Log In Sign up
The largest database of trusted experimental protocols

Salubrinal

Manufactured by Enzo Life Sciences
Visit Supplier
Sourced in United States

Salubrinal is a laboratory reagent produced by Enzo Life Sciences. It is a small molecule that functions as an inhibitor of the protein phosphatase 1 (PP1) complex. Salubrinal acts to regulate cellular stress response pathways.

Automatically generated - may contain errors

Lab products found in correlation

Dihydrotestosterone dht, by Steraloids (1 mentions) Rwpe 1, by Merck Group (1 mentions) Cyclosporin a csa, by Cell Signaling Technology (1 mentions) Bafilomycin a1, by Merck Group (1 mentions) Anti chop, by Cell Signaling Technology (1 mentions) Z vad fmk, by Beyotime (1 mentions) Anti β actin antibody, by Santa Cruz Biotechnology (1 mentions) Anti grp78, by Cell Signaling Technology (1 mentions) Anti dr5, by Cell Signaling Technology (1 mentions) Anti phosphorylated eif2α, by Cell Signaling Technology (1 mentions) Anti caspase 7, by Cell Signaling Technology (1 mentions) Anti parp, by Cell Signaling Technology (1 mentions) Cycloheximide (chx), by Santa Cruz Biotechnology (1 mentions) Puromycin, by MP Biomedicals (1 mentions) Anti ubiquitin, by Cell Signaling Technology (1 mentions) Anti p mdm2, by Cell Signaling Technology (1 mentions) Anti atf6, by Cell Signaling Technology (1 mentions) Anti bip, by Cell Signaling Technology (1 mentions) Anti xbp1, by Cell Signaling Technology (1 mentions) Anti pdi, by Cell Signaling Technology (1 mentions)

4 protocols using salubrinal

1

Prostate Cancer Cell Line Cultivation

Check if the same lab product or an alternative is used in the 5 most similar protocols
LNCaP, and DU145 human prostate cancer cells as well as RWPE-1 immortalized normal prostate epithelial cells were purchased from the American Type Culture Collection (Manassas, VA). LNCaP C4-2B (C42B) cells were purchased from the MD Anderson Cancer Center (Houston, TX). LNCaP, C42B, DU145, and RWPE-1 cells were grown in RPMI 1640 supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 1% HEPES, 1% sodium-pyruvate and 10ml/L of 100x antibiotic-antimycotic solution (Sigma-Aldrich, St-Louis, MO). Cells were maintained in a humidified atmosphere (5% CO2) at 37oC. Ring-substituted 4,4′- and 7,7′-dihaloDIMs were synthesized in our laboratories at >95% purity and were dissolved in 100% dimethyl sulfoxide (DMSO) to obtain 100 mM stock solutions. Dihydrotestosterone (DHT; Steraloids Inc., Newport, RI) was dissolved in DMSO to make a 100 mM stock solution. Cyclosporin A (CsA; Cell Signaling, Beverly, MA), salubrinal (Enzo Life Sciences, Farmingdale, NY), KN92, KN93 (Millipore, Billerica, MA) and bafilomycin A1 (Sigma Aldrich) were dissolved in DMSO as 1000-fold concentrated stock solutions. The final concentration of DMSO in culture medium was 0.1% for single exposures and not greater than 0.3% for combined exposures.
Goldberg A.A., Draz H., Montes-Grajales D., Olivero-Verbél J., Safe S.H, & Sanderson J.T. (2015). 3,3′-Diindolylmethane (DIM) and its ring-substituted halogenated analogs (ring-DIMs) induce differential mechanisms of survival and death in androgen-dependent and –independent prostate cancer cells. Genes & Cancer, 6(5-6), 265-280.
+ Open protocol
+ Expand
2

Salubrinal and TRAIL-Induced Apoptosis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Salubrinal was purchased from Enzo Life Sciences Inc. (San Diego, CA, USA) and dissolved in DMSO to 10 μM and stored at −20 °C. Recombinant Human TRAIL/Apo2L was purchased from PeproTech Inc. (Rocky Hill, NJ, USA) and prepared by reconstituting in deionized water and by diluting in Dulbecco's Minimal Essential Medium (DMEM) to 50 μg/ml and stored at −20 °C. The caspase inhibitor z-VAD-fmk was purchased from Beyotime Institute of Biotechnology (Nantong, Jiangsu, China). Anti-GRP78, anti-DR5, anti-phosphorylated eIF2α, anti-CHOP, anti-caspase-7 and anti-PARP antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-GADD34 antibody was purchased from GeneTex Inc. (Irvine, CA, USA). Anti-Bim antibody was purchased from Epitomics Inc. (Burlingame, CA,USA). Anti-β-actin antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Teng Y., Gao M., Wang J., Kong Q., Hua H., Luo T, & Jiang Y. (2014). Inhibition of eIF2α dephosphorylation enhances TRAIL-induced apoptosis in hepatoma cells. Cell Death & Disease, 5(2), e1060-.
+ Open protocol
+ Expand
3

Comprehensive Protein Regulation Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cycloheximide was from Santa Cruz Biotechnology. Actinomycin-D was from Sigma. MG132 was from Selleck Chemical. Puromycin was from MP Biomedicals. Kainic acid and Thapsigargin were from Alomone Lab. Pifithrin-α was from Adipogen Corporation. Salubrinal was from Enzo Life Sciences. Dimethyl sulfoxide (DMSO) was from Fisher Scientific. DMSO was used as a vehicle in this study. The antibodies used in this study were purchased from Santa Cruz Biotechnology (anti-Mdm2, anti-Lamin A/C, anti-HSP-90), GenScript Corporation (anti-Gapdh), Millipore (anti-Puromycin), and Cell Signaling (anti-ubiquitin, anti-p-Mdm2, anti-p53, anti-BiP, anti-PDI, anti-PERK, anti-ATF6, anti-XBP1s, anti-eIF2α and anti-p-eIF2α). HRP-conjugated secondary antibodies were from Santa Cruz Biotechnology and Cell Signaling. The lentiviral control non-target shRNA and p53 shRNA were from Santa Cruz Biotechnology.
Liu D.C., Eagleman D.E, & Tsai N.P. (2019). Novel roles of ER stress in repressing neural activity and seizures through Mdm2- and p53-dependent protein translation. PLoS Genetics, 15(9), e1008364.
+ Open protocol
+ Expand
4

Pharmacological Modulation of ER Stress Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tauroursodeoxycholic acid (TUDCA; Sigma T0266), was dissolved in PBS and administered at 250 mg/kg body weight. TUDCA was administered to mice via intraperitoneal injection twice a day for 3 days. On the 4th day, mice were treated in the morning and euthanized 4 hours after the last treatment. Control mice were treated with PBS. Salubrinal (Enzo ALX-270-428) was dissolved in dimethyl sulfoxide (DMSO) at 50 mg/ml and diluted 1:400 in PBS. Mice were treated with 1 mg Salubrinal/kg body weight via intraperitoneal injection once a day for 3 days before being euthanized. Thapsigargin (Sigma T9033), was dissolved in ethanol at 10 mg/ml and then diluted 1:40 in PBS. Thapsigargin (2.5 mg/kg body weight) was administered to mice via intraperitoneal injection once a day for 2 days and mice were euthanized 4 hours after the last treatment. Control mice were treated with ethanol diluted 1:40 in PBS. AA147 (2 mg/kg body weight) was administered to mice via intraperitoneal injection twice a day for 3 days and mice were euthanized 4 hours after the last treatment. IL-22-Fc (Genentech, 100ug per dose) was administered once a day for 4 days.
Naama M., Telpaz S., Awad A., Ben-Simon S., Harshuk-Shabso S., Modilevsky S., Rubin E., Sawaed J., Zelik L., Zigdon M., Asulin N., Turjeman S., Werbner M., Wongkuna S., Feeney R., Schroeder B.O., Nyska A., Nuriel-Ohayon M, & Bel S. (2023). Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stress. Cell Host & Microbe, 31(3), 433-446.e4.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!