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5 m pore size transwells

Manufactured by Corning
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The 5-µm pore size transwells are a versatile and reliable laboratory equipment designed for cell culture applications. These transwells feature a polycarbonate membrane with a pore size of 5 micrometers, which allows for the study of cellular interactions, permeability, and transport processes. The core function of these transwells is to provide a barrier between two compartments, enabling researchers to investigate the movement of cells, molecules, or other substances across this barrier.

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Lab products found in correlation

Cxcl12, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Transwell chambers with 5-µm pore size polycarbonate filters Pore transwells

2 protocols using 5 m pore size transwells

1

Transwell Migration Assay for MM Cells

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Migration assay was performed in triplicates using 5-µm pore size transwells (Costar). The lower compartment was filled with 600 µl of 1% FCS RPMI 1640 medium containing CXCL12 (200 ng/ml) (PeproTech EC), and 5 × 105 MM cells in 100 µl of 1% FCS RPMI1640 medium were applied to the upper compartment. The number of cells migrating within 4 h to the lower compartment was determined by FACS and expressed as a percentage of the input.
Beider K., Voevoda-Dimenshtein V., Zoabi A., Rosenberg E., Magen H., Ostrovsky O., Shimoni A., Weiss L., Abraham M., Peled A, & Nagler A. (2022). CXCL13 chemokine is a novel player in multiple myeloma osteolytic microenvironment, M2 macrophage polarization, and tumor progression. Journal of Hematology & Oncology, 15, 144.
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2

CXCL12-Induced Migration of MM Cells

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Migration of MM cell lines in response to various CXCL12 concentrations (5–500 ng/ml) (PeproTech EC) was evaluated using 5-µm pore size transwells (Costar). The quantity of cells migrating within four hours to the lower compartment was determined by FACS and expressed as a percentage of the input. For TRPV1 inhibition, cells were pre-treated (30 min in 37 °C) with AMG9810 (10 µM) and subjected to migration.
Beider K., Rosenberg E., Dimenshtein-Voevoda V., Sirovsky Y., Vladimirsky J., Magen H., Ostrovsky O., Shimoni A., Bromberg Z., Weiss L., Peled A, & Nagler A. (2020). Blocking of Transient Receptor Potential Vanilloid 1 (TRPV1) promotes terminal mitophagy in multiple myeloma, disturbing calcium homeostasis and targeting ubiquitin pathway and bortezomib-induced unfolded protein response. Journal of Hematology & Oncology, 13, 158.
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