DNA was isolated from blood samples with the Genomic DNA Mini Kit (blood/cultured cell) (Geneaid Biotech Ltd., Taiwan) following the manufacturer’s protocol. The quality of extracted DNAs (A260/A280 and A260/A230 ratios) was checked with a Nanodrop 8000c Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).
Nanodrop 8000c spectrophotometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Nanodrop 8000c Spectrophotometer is a laboratory instrument designed for the quantification and analysis of nucleic acids and proteins. It utilizes microvolume sample loading and UV-Vis spectroscopy to measure the absorbance of small-volume samples in the range of 220-750 nm. The Nanodrop 8000c is capable of handling up to 8 samples simultaneously.
Automatically generated - may contain errors
Lab products found in correlation
Genomic dna mini kit, by Geneaid (1 mentions)
Radioimmunoprecipitation assay buffer, by Boston BioProducts (1 mentions)
Halt proteinase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)
Clarity western ecl blotting substrate, by Bio-Rad (1 mentions)
Anti tubulin, by Merck Group (1 mentions)
Anti gapdh, by Thermo Fisher Scientific (1 mentions)
Hur antibody, by Santa Cruz Biotechnology (1 mentions)
Image lab software, by Bio-Rad (1 mentions)
Chemidoc mp imaging system, by Bio-Rad (1 mentions)
Qiaamp dna stool mini kit, by Qiagen (1 mentions)
3 protocols using nanodrop 8000c spectrophotometer
1
Genomic DNA Isolation from Blood
2
Quantitative Western Blotting Analysis
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For Western blotting, whole‐cell lysates were prepared using radioimmunoprecipitation assay buffer (Boston Bio Products, Ashland, MA) with Halt proteinase inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA). Protein concentration was measured using a BCA Protein Assay kit (Pierce, Rockford, MA) and a Nanodrop 8000c spectrophotometer (Thermo Fisher Scientific, Waltham, MA). Equal amounts (30 μg) of proteins were separated onto 4% to 10% mini‐PROTEAN TGX gels (Biorad, Hercules, CA) under reducing and denaturing conditions and transferred to polyvinylidene difluoride membranes. The membranes were blotted with a 1:200 dilution of HuR antibody (Santa Cruz Biotechnology, Santa Cruz, CA) at 4°C overnight. An anti‐GAPDH (1:5000 dilution; Thermo Fisher Scientific, Waltham, MA) or an anti‐tubulin (Sigma) antibody was used as a loading control for all studies. Goat anti‐rabbit or anti‐mouse horseradish peroxidase antibodies (Santa Cruz Biotechnology) were used at a dilution of 1:5000. Proteins were visualized using Clarity Western ECL Blotting Substrate (Biorad). Band intensities were detected by ChemiDoc MP Imaging System and analyzed by Image Lab software (Biorad).
3
Stool DNA Extraction and Quantification
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DNA was extracted from stool samples using the QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. The concentration (ng/µL) and purity (A260/A280 and A260/A230 ratios) of the DNA samples were determined by Nanodrop 8000c Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).
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