Axio observer a1

Manufactured by Zeiss

Sourced in Germany, United States, United Kingdom, Japan, Poland, China

The Axio Observer A1 is a compact and versatile inverted microscope designed for routine observation and imaging. It features a stable frame, adjustable viewing angle, and a range of objectives to accommodate various sample types. The Axio Observer A1 provides a straightforward and efficient solution for basic microscopy applications.

Automatically generated - may contain errors

Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Merck Group (34 mentions) Lsm 710, by Zeiss (30 mentions) Ethd 1, by Thermo Fisher Scientific (18 mentions) Powershot camera, by Canon (16 mentions) Matlab, by MathWorks (15 mentions) Axiovision software, by Zeiss (15 mentions) Axiovision 4, by Zeiss (15 mentions) Live dead cell imaging kit, by Thermo Fisher Scientific (12 mentions) Oil red o, by Merck Group (12 mentions) Axiocam icm1, by Zeiss (12 mentions) Evo ls15, by Zeiss (12 mentions) Matrigel, by BD (12 mentions) Fura 2 am, by Thermo Fisher Scientific (12 mentions) Arrow tl1, by NanoWorld (10 mentions) Axiocam mrm, by Zeiss (10 mentions) Axiocam hrm, by Zeiss (9 mentions) Fluoromount aqueous mounting medium, by Merck Group (9 mentions) Cm1850, by Leica (9 mentions) Axiovision, by Zeiss (9 mentions) Metafluor, by Molecular Devices (9 mentions)

Close spelling variants of this product

Axio-observer A1 fluorescent microscope (6 citations) Axio Observer A1 inverted light microscope (2 citations) Axio Observer A1 inverted microscope (52 citations) Axio Observer A1 fluorescence microscope (27 citations) Axio 154 Observer A1 (5 citations) Axio Observer A1 inverted (13 citations) Axio Observer A1 fluorescence (2 citations) Axio Observer A1—Obj. 20X (2 citations) Axio Observer A1 microscope (144 citations) Axio Observer A1/D1/Z1 (2 citations)

540 protocols using axio observer a1

Fluorescent Microscopic Analysis of Mitochondrial Function and Oxidative Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell were washed with PBS and fixed with 4% paraformaldehyde supplemented with 0.25% Tris-X100 at room temperature for 10 min. After blocking with PBS supplemented with 5% BSA for 2 h at room temperature, cells were incubated with Mito-Tracker Red CMXRos (C1035, Beyotime), NLRP3 (sc-66846, 1:500, Santa Cruz, USA), at 4° C overnight. Cells were incubated with secondary peroxidase conjugated goat anti-rabbit IgG (1:100, Santa Cruz Biotechnology) antibody for 2 h at room temperature, after washing with PBST for 15 min. Cells were stained with DAPI for 15 min at darkness, after washing with PBST for 15 min. Cell samples were observed using fluorescence microscope (Zeiss Axio Observer A1, Germany).
Next, cells were washed with PBS and stained with Mito-Tracker Red CMXRos for 30 min. Cells washed with PBST for 15 min and stained with DCFH-DA for 30 min. Cell samples were observed using fluorescence microscope (Zeiss Axio Observer A1, Germany) after washing with PBST for 15 min.
Cell were washed with PBS and fixed with 4% paraformaldehyde for 15 min. Cells were stained with PI for 15 min at darkness, after washing with PBST for 15 min. Cell samples were observed using fluorescence microscope (Zeiss Axio Observer A1, Germany).

Zhang W., Wang W., Shen C., Wang X., Pu Z, & Yin Q. (2021). Network pharmacology for systematic understanding of Schisandrin B reduces the epithelial cells injury of colitis through regulating pyroptosis by AMPK/Nrf2/NLRP3 inflammasome. Aging (Albany NY), 13(19), 23193-23209.

+ Open protocol

+ Expand

Multilineage Differentiation of Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were seeded in 24-well plates at a concentration of 2 × 10⁴ cells per well and subsequently adipogenic, chondrogenic, and osteogenic differentiations were performed. Stimulation of MSCs was performed using StemPro Adipogenesis, Chondrogenesis, and Osteogenesis Differentiation Kits (Life Technologies, Poland) (StemPro Adipogenesis Differentiation Kit, StemPro Chondrogenesis Differentiation Kit, and StemPro Osteogenesis Differentiation Kit) according to the manufacturers' instruction. Differentiations began 24 h after inoculation and cells were cultured for 14 days. Media were changed every three days. The stage of adipogenic, chondrogenic, and osteogenic differentiation of MSCs was assessed using specific staining: Oil Red O (Sigma-Aldrich, Munich, Germany) for detecting neutral lipid deposits, Safranin O (Sigma-Aldrich, Munich, Germany) for glycosaminoglycans staining, and Alizarin Red (Sigma-Aldrich, Munich, Germany) for calcium deposits. Preparations were analyzed using Axio Observer A1 inverted microscope (Axio Observer A1, Carl Zeiss, Jena, Germany), while the documentation was made using Cannon PowerShot camera.

Marędziak M., Śmieszek A., Chrząstek K., Basinska K, & Marycz K. (2015). Physical Activity Increases the Total Number of Bone-Marrow-Derived Mesenchymal Stem Cells, Enhances Their Osteogenic Potential, and Inhibits Their Adipogenic Properties. Stem Cells International, 2015, 379093.

+ Open protocol

+ Expand

Differentiation of Adipose-Derived Stem Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Osteogenic and adipogenic differentiation of ASCs were induced using commercial kits (StemPro, Life Technologies). Cultures under standard and osteo- and adipogenic conditions were maintained in 24-well dishes. Experiments were carried out simultaneously, each in triplicate according to the previous findings [33 (link)]. Osteogenesis was induced during 21-day period, while stimulation toward adipocytes lasted for 14 days. To evaluate results of osteogenic and adipogenic differentiation, two specific staining methods were used, that is, Alizarin Red for determination of calcium deposits and detection of neutral lipid deposits performed using HCS LipidTOX Green neutral lipid stain, and according to the manufacturers' instruction preparations were analyzed using Axio Observer A1 inverted and epifluorescent microscope (Axio Observer A1, Carl Zeiss, Jena, Germany), while the documentation was made using Cannon PowerShot camera.

Śmieszek A., Basińska K., Chrząstek K, & Marycz K. (2015). In Vitro and In Vivo Effects of Metformin on Osteopontin Expression in Mice Adipose-Derived Multipotent Stromal Cells and Adipose Tissue. Journal of Diabetes Research, 2015, 814896.

+ Open protocol

+ Expand

Kidney Tissue Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Kidney tissue samples were fixed in 4% paraformaldehyde, paraffin-embedded, and then sectioned into 5 μM slices for Masson staining, PAS staining, or TUNEL staining. Kidney tissue samples were observed using fluorescence microscope (Zeiss Axio Observer A1, Germany).
Kidney slices were used for collagen IV by immunohistochemistry. Kidney slices are incubated with primary antibodies against collagen IV (CST, USA) at 1 : 200 dilution overnight at 4°C with the appropriate secondary antibodies (1 : 200 Santa Cruz, CA, USA). Kidney tissue samples were observed using fluorescence microscope (Zeiss Axio Observer A1, Germany).

Wang X., Li Q., Sui B., Xu M., Pu Z, & Qiu T. (2022). Schisandrin A from Schisandra chinensis Attenuates Ferroptosis and NLRP3 Inflammasome-Mediated Pyroptosis in Diabetic Nephropathy through Mitochondrial Damage by AdipoR1 Ubiquitination. Oxidative Medicine and Cellular Longevity, 2022, 5411462.

+ Open protocol

+ Expand

Stomatal Morphology Microscopic Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Three to five leaves were randomly sampled from tomato plants from each treatment in order to observe stomatal morphology. Prior to microscopic observation, the abaxial leaf epidermis was removed with tweezers and incubated in distilled water. Stomatal apertures were observed with an Axio Observer A1 inverted fluorescence microscope (Zeiss, Oberkochen, Germany). Approximately six random photos were taken for each leaf.

Yang X., Zhang Y., Liu T., Shi J., Qi M., Liu Y, & Li T. (2022). Integrated Physiological, Transcriptomic, and Proteomic Analyses Reveal the Regulatory Role of Melatonin in Tomato Plants’ Response to Low Night Temperature. Antioxidants, 11(10), 2060.

+ Open protocol

+ Expand

Microfluidic Platelet Formation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Microfluidic flow chamber chips were manufactured from polydimethylsiloxane as described before 27. One chip (length = 17·3 cm) consisted of 16 parallel channels composed of a structured network of pillars. The radius of one pillar is 15 μm, and the distance between neighbouring pillars, from centre to centre, is 85 μm. The pillars are oriented so that the angle between the pillar array and the flow direction of the channel is 10°. Prior to cell perfusion, the chip was coated overnight with 40 μg/ml purified VWF (Wilfactin, LFB Biotechnologies, France). The perfusion experiment was performed according to Blin et al. 27. In brief, the harvested cell suspension on D12 of culture was placed on an orbital shaker and was connected to the perfusion chamber inlet and outlet by tubing. A peristaltic pump (Watson‐Marlow, Falmouth, UK) created continuous perfusion of the cell suspension at a wall shear rate of 1800 s⁻¹ for two hours during which platelets were formed. Adhering cells were visualized by phase contrast microscopy at 100× magnification (Axio observer A1, Carl Zeiss). After perfusion, the suspension was centrifuged at 110 g for 20 min to remove MK. Next, the supernatant was centrifuged at 1240 g for 15 min to pellet the platelets.

Six K.R., Sicot G., Devloo R., Feys H.B., Baruch D, & Compernolle V. (2019). A comparison of haematopoietic stem cells from umbilical cord blood and peripheral blood for platelet production in a microfluidic device. Vox Sanguinis, 114(4), 330-339.

+ Open protocol

+ Expand

Imaging cells with phase-contrast microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols

Phase-contrast microscopy was performed using a Zeiss Axio Observer A1 inverted microscope (Zeiss, Germany) equipped with Q-Capture software (QImaging, Surrey, Canada).

Ali M., Kabir F., Thomson J.J., Ma Y., Qiu C., Delannoy M., Khan S.Y, & Riazuddin S.A. (2019). Comparative transcriptome analysis of hESC- and iPSC-derived lentoid bodies. Scientific Reports, 9, 18552.

+ Open protocol

+ Expand

Quantifying Cellular Senescence via SA-β-Gal

Check if the same lab product or an alternative is used in the 5 most similar protocols

SA-β-gal activity is a widely used biomarker of cellular senescence [26 (link)] and was thus applied to identify KGN cell and mouse GC senescence. KGN cells and mGCs were seeded on 12-well plates at a density of 5^∗10⁵ cells/ml, followed by being washed with PBS and fixed and stained with X-gal solution (C0602, Beyotime Biotechnology) overnight at 37°C. Cells were imaged and photographed using an inverted microscope (Axio Observer A1, Zeiss, Germany).

Lin N., Lin J., Plosch T., Sun P, & Zhou X. (2022). An Oxidative Stress-Related Gene Signature in Granulosa Cells Is Associated with Ovarian Aging. Oxidative Medicine and Cellular Longevity, 2022, 1070968.

+ Open protocol

+ Expand

Quantifying Vascular Nitric Oxide

Check if the same lab product or an alternative is used in the 5 most similar protocols

4-Amino-5-Methylamino-2′,7′-Difluorofluorescein (DAF-FM) diacetate reagent was used to detect and quantify low concentrations of vascular nitric oxide (NO). Briefly, isolated aortae were embedded in OCT compound (Sakura Finetik, AJ Alphen aan den Rijn, Netherlands) and kept frozen. The frozen segments were cut into cryostat sections of 10 μm thick. The cut sections were then incubated with 5 μM DAF-FM diacetate (Invitrogen, Carlsbad, CA, USA) for 15 min in normal physiological saline solution [NPSS (mM): NaCl 140, KCl 5, CaCl₂ 1, MgCl₂ 1, glucose 10 and HEPES 5] at 37 °C. The fluorescence dye was then washed and the sections were visualized using a fluorescence microscope (Zeiss Axio Observer A1) with excitation at 495 nm and emission at 515 nm. The fluorescence intensity was measured using the Zen Lite software version 2.3. The NO level was compared to that of the control.

Mohd Sabri N.A., Lee S.K., Murugan D.D, & Ling W.C. (2022). Epigallocatechin gallate (EGCG) alleviates vascular dysfunction in angiotensin II-infused hypertensive mice by modulating oxidative stress and eNOS. Scientific Reports, 12, 17633.

+ Open protocol

+ Expand

Imaging Mouse Brain Ependymal Cilia Motility

Check if the same lab product or an alternative is used in the 5 most similar protocols

For preparation of mouse brain ependymal cells, mouse were anesthetized and perfused using Isoflurane (Hana pharm, Gyeounggi) and PBS respectively. Brain is extracted from the mouse and placed in ice-cold PBS. The brain in PBS is cut into coronal sections with 100 μm using vibratome (Leica, Germany) and transferred into confocal plate (SPL Life Science; Pocheon, Korea, #100,351). For preparation of imaging organoids, basal oragnoids embedded in matrigel were laid on the confocal plate with transwell inserts. Motility of cilia was examined (100 frames/second) with a Axio Observer A1 (Carl Zeiss, Germany) and motion pattern of cilia was traced with NIS-Elements software (Nikon, Japan).

Kim D.Y., Sub Y.J., Kim H.Y., Cho K.J., Choi W.I., Choi Y.J., Lee M.G., Hildebrandt F, & Gee H.Y. (2023). LRRC6 regulates biogenesis of motile cilia by aiding FOXJ1 translocation into the nucleus. Cell Communication and Signaling : CCS, 21, 142.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!