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Ab56400

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Ab56400 is a laboratory equipment product. It is designed for general laboratory use.

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Lab products found in correlation

Gapdh d16h11, by Cell Signaling Technology (1 mentions) Active β catenin clone 8e7, by Merck Group (1 mentions) Ab16048, by Abcam (1 mentions) Anti stat3 124h6, by Cell Signaling Technology (1 mentions) Anti ki 67, by Lab Vision (1 mentions) Ab111691, by Abcam (1 mentions) Ab205606, by Abcam (1 mentions) Ab54217, by Abcam (1 mentions) Ab270264, by Abcam (1 mentions) Ab231036, by Abcam (1 mentions) Ab17147, by Abcam (1 mentions) Ab92381, by Abcam (1 mentions) Ab236632, by Abcam (1 mentions) Ab213204, by Abcam (1 mentions) Ab210797, by Abcam (1 mentions) Ab217344, by Abcam (1 mentions)

3 protocols using ab56400

1

Quantitative Western Blot Analysis of Apoptosis and Wnt Signaling

Cited 1 time
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Western blot of whole protein lysates or nuclear proteins from frozen tumor tissues or cells was performed as previously described (24 (link)). Antibodies for cleaved caspase 3 (Asp175) (5A1E), cleaved PARP (Asp214) (D64E10), cyclin D1 (92G2), β-catenin (6B3), active β-catenin (D13A1), TCF4 (C48H11), axin 1 (C95H11), tubulin (DM1A) and GAPDH (D16H11) were purchased from Cell Signaling Technology. Other antibodies included lamin B1 (ab16048, Abcam), COP1 (ab56400, Abcam), tankyrase1/2 (H-350, Santa Cruz), and active β-catenin (Clone 8E7, Millipore). ImageJ software was used to measure the relative density for signaling expression.
Zeng S., Seifert A.M., Zhang J.Q., Cavnar M.J., Kim T.S., Balachandran V.P., Santamaria-Barria J.A., Cohen N.A., Beckman M.J., Medina B., Rossi F., Crawley M.H., Loo J.K., Maltbaek J.H., Besmer P., Antonescu C.R, & DeMatteo R.P. (2017). Wnt/β-catenin signaling contributes to tumor malignancy and is targetable in gastrointestinal stromal tumor. Molecular cancer therapeutics, 16(9), 1954-1966.
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2

Immunohistochemical Analysis of Signaling Proteins

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IHC was performed as previously described14 (link) using the following Ab: anti-STAT3 (124H6; Cell Signaling; catalog 9139); anti-p-STAT3 Tyr705 (D3A7; Cell Signaling; catalog 9145); anti-COP1 (ab56400, Abcam); anti-c-Jun (E254, ab32137); anti-EZH2 (D2C9, CST#5246); anti-Ki67 (Lab Vision Corp.; ready-to-use RT-9106-R7). The specificity of all antibodies was previously confirmed by Western blot analysis. Cell nuclei were counterstained with hematoxylin solution. Slides were evaluated by at least two investigators in a blinded manner.
Albino D., Falcione M., Uboldi V., Temilola D.O., Sandrini G., Merulla J., Civenni G., Kokanovic A., Stürchler A., Shinde D., Garofalo M., Mestre R.P., Constâncio V., Wium M., Burrello J., Baranzini N., Grimaldi A., Theurillat J.P., Bossi D., Barile L., Henrique R.M., Jeronimo C., Zerbini L.F., Catapano C.V, & Carbone G.M. (2021). Circulating extracellular vesicles release oncogenic miR-424 in experimental models and patients with aggressive prostate cancer. Communications Biology, 4, 119.
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3

Comprehensive Immunophenotyping Assay

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The following primary antibodies were used: anti-tubulin (Abcam, ab210797), anti-KMT5A (Abcam, ab111691), anti-CD8 (Abcam, ab17147), anti-CD8 (Abcam, ab217344), anti-CD3 (Abcam, ab16669), anti-ubiquitin (CST, #43124), anti-CD69 (Abcam, ab54217), anti-FLAG (Abcam, ab205606), anti-HA (Abcam, ab236632), anti-6X His (Abcam, ab213204), anti-CD73 (Abcam, ab54217), anti-CD73 (Abcam, ab54217), anti-COP1 (Abcam, ab56400), anti-MKRN1 (Abcam, ab72054), anti-MDM2 (BOSTER, BA3612–2), and anti-Ki-67 (CST, #12202), anti-CD69 (BOSTER, A00529–2), anti-IFN-γ (Abcam, ab231036), anti-IL-2 (Abcam, ab92381), anti-TNF-α (Abcam, ab270264), anti-perforin (Abcam, ab47225), anti-Granzyme B (BOSTER, A00353), anti-perforin (Abcam, ab16074). The following secondary antibodies were used: goat anti-mouse (CST) and goat anti-rabbit (CST). MG132 and cycloheximide (CHX) were purchased from CST (USA). The CMV peptide pool stimulating CD8+ T cells was obtained from Mabtech (Sweden).
Xu Y., Liu Z., Lv L., Li P., Xiu B., Qian W, & Liang A. (2020). MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8+ T lymphocytes in human diffuse large B cell lymphoma. Journal of Experimental & Clinical Cancer Research : CR, 39, 238.
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