Nutlin-3 (rel-4-[[(4R,5S)-4,5-bis(4-chlorophenyl)-4,5-dihydro-2-[4-methoxy-2-(1-methylethoxy)phenyl]-1H-imidazol-1-yl]carbonyl]-2-piperazinone) was purchased from Cayman Chemicals (Ann Arbor, MI, USA). Nutlin-3a ((-)-4-(4,5-bis(4-chlorophenyl)-2-(2-isopropoxy-4-methoxyphenyl)-4,5-dihydro-1H-imidazole-1-carbonyl) piperazin-2-one, NUT, 98% ee) was synthesized following the procedure reported in the literature.14 (link),27 (link) The soybean lecithin PC (94% phosphatidylcholine) was Epikuron 200 from Lucas Meyer (Hamburg, Germany). Polytetrafluoroethylene (PTFE) membranes (pore size 0.22 μm), and 5(6)-Carboxy-X-rhodamine (Rho) were purchased from Merck-Sigma Aldrich (Milan, Italy). Solvents were of HPLC grade, and all other chemicals were of analytical grade.
Polytetrafluoroethylene ptfe membranes
Manufactured by Merck Group
Sourced in Italy, United States, Germany
Polytetrafluoroethylene (PTFE) membranes are a type of laboratory equipment used for various filtration and separation applications. PTFE, also known as Teflon, is a chemically inert and thermally stable material that is highly resistant to a wide range of chemicals and solvents. PTFE membranes are designed to provide efficient and reliable filtration, separation, and purification of liquids and gases.
Automatically generated - may contain errors
Lab products found in correlation
Nutlin 3, by Cayman Chemical (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Polysorbate 80, by Merck Group (1 mentions)
Polysorbate 20, by Merck Group (1 mentions)
Sorbitan monolaurate, by Merck Group (1 mentions)
Polyoxyethylene sorbitan monooleate, by Merck Group (1 mentions)
Polyoxyethylenesorbitan monolaurate, by Merck Group (1 mentions)
Congo red, by Merck Group (1 mentions)
Sodium cholate, by Merck Group (1 mentions)
Mas 100 eco aeroscope, by Merck Group (1 mentions)
4 protocols using polytetrafluoroethylene ptfe membranes
1
Nutlin-3 Synthesis and Characterization
2
Mangiferin-Based Nanoformulations for DPPH Assay
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Mangiferin, Mangifera indica, 1,3,6,7-Tetrahydroxyxanthone C2-β-D-glucoside (MG), polyoxyethylenesorbitan monolaurate, polysorbate 20 (TW20), polyoxyethylenesorbitan monooleate, polysorbate 80 (TW80), sorbitan monolaurate (SP20), sorbitane monooleate (SP80), dimethyldidodecylammonium bromide (DDAB), 2,2-Diphenyl-1-picrylhydrazyl (DPPH), nylon, mixed cellulose esters (MCE), and polytetrafluoroethylene (PTFE) membranes (diam. 25 mm, pore size 0.2 μm) were purchased from Merck, Sigma-Aldrich (Santa Louis, MO, USA). The soybean lecithin (90% phosphatidylcholine) (PC) was Epikuron 200 from Lucas Meyer, Hamburg, Germany. Solvents were HPLC grade and all other chemicals were analytical grade.
3
Purification of Single-Wall Carbon Nanotubes
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Single-wall carbon nanotubes (SWNTs, purity >90%, length 700 nm, diameter 0.7–0.9 nm), Congo red (CR, 96% purity) and sodium cholate were obtained from Sigma. Polytetrafluoroethylene (PTFE) membranes (0.2 µm pore size) were purchased from MERCK Millipore. All other reagents used were of analytical grade.
4
Standardized Sampling of Audio-Visual Materials
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Preparation for sampling and the sampling process were the same as described in our previous study (10 (link)). Before sampling, instruments such as tweezers, scalpels, and scissors were exposed to gamma radiation for sterilization. Similarly, 0.8% saline solution from water for molecular microbiology was also exposed to gamma radiation. Polytetrafluoroethylene (PTFE) membranes with a porosity of 0.22 μm and a diameter of 90 mm (Merck Millipore, Germany) were sterilized using an autoclave and subsequently illuminated with a UV lamp.
Proportional swabbing of each audio-visual material was carried out to ensure consistency in the sampling approach. The method for sampling the materials was chosen to be as gentle to the material as possible. Thus, sterile polyurethane sponges (World Bio-Products, Woodinville, WA, USA) were used. Air sampling was conducted at a height of approximately 1 m within the shelves, precisely where the audio-visual materials were stored. The sampling locations varied, ranging from the central area of the room to shelves positioned near the wall. An active intake of 3,000 liters of air onto PTFE Fluoropore membranes using a MAS-100 Eco aeroscope (Merck Millipore, Germany) was used for air sampling. The swabs and the PTFE membranes were stored at −20°C for further analysis.
Proportional swabbing of each audio-visual material was carried out to ensure consistency in the sampling approach. The method for sampling the materials was chosen to be as gentle to the material as possible. Thus, sterile polyurethane sponges (World Bio-Products, Woodinville, WA, USA) were used. Air sampling was conducted at a height of approximately 1 m within the shelves, precisely where the audio-visual materials were stored. The sampling locations varied, ranging from the central area of the room to shelves positioned near the wall. An active intake of 3,000 liters of air onto PTFE Fluoropore membranes using a MAS-100 Eco aeroscope (Merck Millipore, Germany) was used for air sampling. The swabs and the PTFE membranes were stored at −20°C for further analysis.
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