Polypropylene conical tube

Manufactured by BD

Sourced in United States, Mexico

Polypropylene conical tubes are laboratory equipment designed for various sample collection, storage, and processing applications. They are made of a durable polypropylene material and feature a conical shape with a rounded bottom. These tubes are commonly used in scientific research, diagnostic testing, and other laboratory settings.

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14 protocols using polypropylene conical tube

LSC Instrumentation and Techniques

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For LSC analysis, we used Tri-Carb 3110 #1 and #2, Tri-Carb 5110, and a Quantulus GCT6220 instrument, and 20-mL LSC plastic vials (all from PerkinElmer). The Quantulus GCT6220, a newer version of the Tri-Carb, has a guard compensation technology (GCT) option that can decrease background interference for alpha and beta nuclides. We used a high-precision analytical balance with an accuracy of 0.0001 gm (Mettler-Toledo, LLC). We also used 15 mL and 50 mL conical polypropylene tubes (Becton Dickinson) for different solution preparation, a Brinkman bottletop dispenser with capacity from 5 mL to 25 mL (Brinkman Instruments, Inc.) for cocktail dispensing, and four electronic pipettes with total volume range from 5 μL to 5 mL (Eppendorf, Inc).

Piraner O, & Jones R.L. (2021). Urine gross alpha/beta bioassay method development using liquid scintillation counting techniques. Journal of radioanalytical and nuclear chemistry, 327(1), 513-523.

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Ultra-Low-Level Liquid Scintillation Spectrometry

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For this study, we used three ultralow level liquid scintillation spectrometers Quantulus1220 (Q#1,2,3) equipped with PSA and MCA (PerkinElmer); 20-mL LSC plastic vials (PerkinElmer) for LSC analysis; a high-precision analytical balance capable of accurately weighing 0.0001 gm (Mettler-Toledo, LLC) for quench curves and urine spikes preparation; 15-mL and 50-mL conical polypropylene tubes (Beckton Dickinson) for solutions preparation; Brinkman bottle top dispenser with capacity from 5 mL to 25 mL (Brinkman Instruments, Inc.) for cocktail dispensing; and four electronic pipettes with a total volume range from 5 μL to 5 mL (Eppendorf, Inc).

Piraner O, & Jones R.L. (2021). Universal use of alpha/beta mode in liquid scintillation counting analysis for both alpha/beta and single nuclide determination. Journal of radioanalytical and nuclear chemistry, 327, 975-983.

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Chondrogenic Differentiation of Canine Cells

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Chondrogenic differentiation of canine chondrocytes and SDSCs at P2 was evaluated in a 28-day micropellet culture study [13] (link). Briefly, 0.5×10⁶ cells were centrifuged (300×g for 7 min) in 15 mL conical polypropylene tubes (Becton Dickinson) and the resulting pellet was cultured in 0.5 mL of chondrogenic medium (hgDMEM, 1% (v/v) PSAM, 1% (v/v) ITS™+Premix (BD Biosciences), 100 µg/mL sodium pyruvate (Sigma), 50 µg/mL L-proline (Sigma-Aldrich), 0.1 µM dexamethasone (Sigma-Aldrich), with 50 µg/mL ascorbate-2-phosphate (Sigma-Aldrich) and 10 ng/mL TGF-ß3 (R&D) added fresh during each media change. SDSC pellets were additionally treated with 500 ng/mL bone morphogenetic protein-2 (BMP-2, GenScript). All chondrocyte and SDSC pellets were fed twice per week.

Alegre-Aguarón E., Sampat S.R., Xiong J.C., Colligan R.M., Bulinski J.C., Cook J.L., Ateshian G.A., Brown L.M, & Hung C.T. (2014). Growth Factor Priming Differentially Modulates Components of the Extracellular Matrix Proteome in Chondrocytes and Synovium-Derived Stem Cells. PLoS ONE, 9(2), e88053.

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Fixation and Staining of LCM Slides

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Fixation and staining of LCM slides was conducted under RNAse-free conditions in 50 ml conical polypropylene tubes (Falcon, Franklin Lakes, NJ, USA) containing 45 ml of solution, as previously described [11 (link)]. ProtectRNA™RNAse inhibitor (1:500) (Sigma, Saint Louis, MO, USA) was incorporated in the LCM staining protocol (Table 1, S3 Fig) to protect RNA from degradation during water containing steps performed at room temperature (RT) [15 (link)].
LCM slides were moved on dry ice from a slide box into fixative that was prechilled on dry ice for 1 hour (fixative reached a temperature of -20°C). In the OCT removal step (Table 1), the solution was applied to sections twice, and the slides were drained on Kimwipes between applications. “One-step Cresyl Violet Acetate / Eosin Y” stain [11 (link)], was modified as follows: 75 μl of cresyl violet stock solution, 25 μl of eosin Y, 250 μl of RNAse-free water and 250 μl of 100% ethanol.

Castro N.P., Merchant A.S., Saylor K.L., Anver M.R., Salomon D.S, & Golubeva Y.G. (2016). Adaptation of Laser Microdissection Technique for the Study of a Spontaneous Metastatic Mammary Carcinoma Mouse Model by NanoString Technologies. PLoS ONE, 11(4), e0153270.

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Dynamic Palate Culture with HA Degradation

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Dynamic palate culture was performed as described previously (Snyder-Warwick et al., 2010 (link)). Briefly, the palate explant including the palate shelves and the attached maxilla was prepared by removing the tongue, mandible, upper cranial and brain tissues from freshly dissected embryonic heads. Palatal explants were placed in 50-mL conical polypropylene tubes (Falcon) filled with 10 mL of BGJb medium containing L-glutamine (Gibco). Penicillin (50 unit/ml) and streptomycin (50 μg/ml) were added into the culture medium but serum was not added. The culture medium was flushed for three minutes with oxygen and the culture tubes were immediately sealed airtight with size 6 solid rubber stoppers and parafilm to maintain the oxygen concentration in the culture tubes. Palate explants were cultured at 37°C with slow rotation (5 rpm) in a hybridization incubator (Robbins Scientific, Model 400) for 48 hours. Palatal shelf morphology was examined after 24-hour and 48-hour culture.
To enhance HA degradation during culture, norchlorcyclizine (NORCHLR) (Sigma) was dissolved in DMSO and added into the culture medium to a final concentration of 100 μg/ml. In the control group, only DMSO was added into the culture medium. The final concentration of DMSO in the culture medium was 0.1%, which did not affect shelf behaviors during culture by comparing with those without DMSO.

Yonemitsu M.A., Lin T.Y, & Yu K. (2019). Hyaluronic acid is required for palatal shelf movement and its interaction with the tongue during palatal shelf elevation. Developmental biology, 457(1), 57-68.

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Synthetic Amyloid Fibril Generation

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To prepare synthetic amyloid fibrils from rVλ6Wil, a 1 mL-volume containing ~1 mg/mL of monomer in phosphate-buffered saline (PBS), 0.01% w/v NaN₃, pH7.5, was filtered through 0.2 µm pore-sized filter, added to a 15 mL conical polypropylene tube (BD BioSciences, Bedford, MA) and shaken at a 45° angle at 225 rpm for 3–5 d at 37 °C until the reaction mixture became opaque [19] (link). For Aβ(1−40) and IAPP fibrils, filtered 1 mL volumes of 0.2 mg/mL peptide in PBS with 0.01% w/v NaN₃, pH7.5, were placed at 37 °C without shaking for 5−10 d. The presence of amyloid fibrils was confirmed by using a thioflavin T (ThT; Sigma-Aldrich, St. Louis, MO) fluorescence emission assay. Briefly, a suspension of fibrils at 50 µg/ mL was prepared and 100 µL (5 µg) of the fibril suspension was added to each of three wells on a 96-well microplate. Thirty µL of PBS was added to each of the wells prior to the addition of 10 µL of 300 µM ThT (Sigma-Aldrich, St. Louis, MO). A set of triplicate wells containing PBS and ThT only was used as a background control. The ThT fluorescence emission (490 nm, excitation at 450 nm) was measured using a fluorescence plate reader (Victor 1420 multilabel counter, Perkin Elmer, Wellesley, MA) and corrected by subtraction of mean background fluorescence. Fibril preparations were aliquoted into single use volumes and stored at −80 °C.

Wall J.S., Williams A., Wooliver C., Martin E.B., Cheng X., Heidel R.E, & Kennel S.J. (2016). Secondary structure propensity and chirality of the amyloidophilic peptide p5 and its analogues impacts ligand binding - In vitro characterization. Biochemistry and Biophysics Reports, 8, 89-99.

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Amyloid Fibril Preparation Protocol

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To prepare synthetic amyloid fibrils from rVλ6Wil, a 1 mL-volume
containing ~ 1 mg/mL of monomer in phosphate-buffered saline (PBS), 0.01% w/v
NaN₃, pH7.5, was filtered through 0.2 μm pore-sized
filter, added to a 15 mL conical polypropylene tube (BD BioSciences, Bedford,
MA) and shaken at a 45° angle at 225 rpm for 3 – 5 d at 37°
C until the reaction mixture became opaque [19 (link)]. For Aβ(1-40) and IAPP fibrils, filtered 1 mL volumes of
0.2 mg/mL peptide in PBS with 0.01% w/v NaN₃, pH7.5, were placed at
37°C without shaking for 5 - 10 d. The presence of amyloid fibrils was
confirmed by using a thioflavin T (ThT; Sigma-Aldrich, St. Louis, MO)
fluorescence emission assay. Briefly, a suspension of fibrils at 50 μg/
mL was prepared and 100 μL (5 μg) of the fibril suspension was
added to each of three wells on a 96-well microplate. Thirty μL of PBS
was added to each of the wells prior to the addition of 10 μL of 300
μM ThT (Sigma-Aldrich, St. Louis, MO). A set of triplicate wells
containing PBS and ThT only was used as a background control. The ThT
fluorescence emission (490 nm, excitation at 450 nm) was measured using a
fluorescence plate reader (Victor 1420 multilabel counter, Perkin Elmer,
Wellesly, MA) and corrected by subtraction of mean background fluorescence.
Fibril preparations were aliquoted into single use volumes and stored at
−80°C.

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Multi-Residue Analysis of Veterinary Drugs

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CAP (99.8% purity, CAS No.: 56-75-7), TAP (99.9% purity, CAS No.: 15318-45-3), FF (99% purity, CAS No.: 73231-34-2), FFA (99.3% purity, CAS No.: 76639-93-5), acetic acid (99.5% purity), ammonium hydroxide solution (NH₄OH), and ethylenediaminetetraacetic acid disodium salt (EDTA) solution (0.5 M in H₂O) were acquired from Sigma-Aldrich Corporation (St. Louis, MO, USA). HPLC-grade methanol (MeOH; 99.9% purity) and acetonitrile (ACN; 100% purity) were purchased from Pharmaco-Aaper (Brookfield, CT, USA) and JT Baker (Phillipsburg, NJ, USA). QuEChERS dSPE kits (containing 150 mg of primary-secondary amine (PSA) and 900 mg of MgSO₄) were obtained from Phenomenex (Torrance, CA, USA). Cellulose acetate membrane filters (0.45 μm) were supplied by MILLEX (Merck Millipore Ltd, Co. Cork, Ireland), and 0.2 μm PTFE syringe filters were sourced from Pall Corporation (Michigan, USA). The polypropylene conical tubes (15 and 50 mL) used throughout the entire experiment were acquired from FALCON (Tamaulipas, Mexico). Ultrapure water (resistivity of 18.2 MΩ.cm at 25°C) was supplied by a Milli-Q water purification system (Millipore, Bedford, MA, USA). All matrices (beef, pork, chicken, shrimp, eel, and flatfish) were procured from local markets in Seoul, Republic of Korea.

Jung H.N., Park D.H., Choi Y.J., Kang S.H., Cho H.J., Choi J.M., Shim J.H., Zaky A.A., Abd El-Aty A.M, & Shin H.C. (2022). Simultaneous Quantification of Chloramphenicol, Thiamphenicol, Florfenicol, and Florfenicol Amine in Animal and Aquaculture Products Using Liquid Chromatography-Tandem Mass Spectrometry. Frontiers in Nutrition, 8, 812803.

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MAIT Cell Activation Assay

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Freshly drawn blood was distributed in 5-mL polypropylene conical tubes (BD Falcon). One milliliter of blood was activated with 5-OP-RU (10 μg/mL) or E. coli at the indicated MOI in the presence or absence of DB28 (100 μg/mL). After overnight stimulation, cells were stained in Brilliant violet buffer (BD) with the following antibodies: BUV661 CD3 (UCHT1; BD), PE/Dazzle CD137 (VI C-7, Biolegend), antigen-presenting cell CD161 (HP-3G10, Biolegend), BV605 Vα7.2 (3C10, Biolegend), and BV510 CD19 (HIB19, Biolegend). Samples were acquired on a ×50 BD Symphony machine and analyzed with FlowJo 10. Viability was assessed with live/dead Aqua staining, according to the manufacturer’s instructions (Thermo Fisher). E. coli (DH5a; Thermo Fisher) was grown overnight in Luria broth medium and after extensive washing in PBS, OD₆₀₀ was measured. An OD₆₀₀ = 1 was considered equivalent to 5 × 10⁸ bacteria/mL

Salio M., Awad W., Veerapen N., Gonzalez-Lopez C., Kulicke C., Waithe D., Martens A.W., Lewinsohn D.M., Hobrath J.V., Cox L.R., Rossjohn J., Besra G.S, & Cerundolo V. (2020). Ligand-dependent downregulation of MR1 cell surface expression. Proceedings of the National Academy of Sciences of the United States of America, 117(19), 10465-10475.

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Stability of Silica Digestates for ICP-OES

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While it is preferable to measure freshly prepared samples, knowing the maximum amount of time a sample can be stored without a significant decrease in Si recovery can help to simplify the planning of laboratory work. We therefore investigated the stability of digestates prepared according to method KOH0.1 over an extended period (2 months). The digestates were stored at RT (21 ± 1 °C) in 50 mL polypropylene conical tubes (Falcon^®, Corning, NY, USA) and measured 1, 14, and 61 days after the digestion. The samples contained Si reference standard solution at 35.6 mmol L⁻¹ (TraceCERT^®, 1000 ± 2 mg L⁻¹ Si in NaOH, Sigma-Aldrich, Switzerland). For the analysis by ICP-OES, the digestates were diluted in an acidic BgS to an expected final Si concentration that fell into the range of the Si calibration (Fig. 5). These diluted digestates were spiked with 500 µg L⁻¹ Y and analyzed within 24 h by ICP-OES.

Bossert D., Urban D.A., Maceroni M., Ackermann-Hirschi L., Haeni L., Yajan P., Spuch-Calvar M., Rothen-Rutishauser B., Rodriguez-Lorenzo L., Petri-Fink A, & Schwab F. (2019). A hydrofluoric acid-free method to dissolve and quantify silica nanoparticles in aqueous and solid matrices. Scientific Reports, 9, 7938.

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