Gibco freestyle 293 expression medium
GIBCO™ FreeStyle™ 293 Expression Medium is a serum-free, chemically defined media designed for the transient transfection and expression of recombinant proteins in 293 cell lines.
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19 protocols using gibco freestyle 293 expression medium
Purification of Recombinant Human FcRn
Purification of Porcine CCL Proteins
Characterization of Engineered Mouse Cell Lines
Efficient Production of Recombinant rIL4-10 Fusion Protein
pcDNA3.1-neo expression vector (Invitrogen, Carlsbad, CA, USA) containing a
dual CMV (cytomegalovirus) promoter. The vector contained 2 transgenes: cDNA
coding for rIL4-10 and cDNA coding β-galactosidealpha-2,3-sialyltransferase
to optimize glycan capping with sialic acid. To enable purification, a
hexa-histidine affinity tag was cloned on the N-terminus of rIL4-10 FP.
Cells were cultured in GIBCO FreeStyle 293 Expression Medium (Invitrogen).
The medium contained no serum or antibiotics. Cells were grown in flasks on
a shaker platform in humidified, 5% CO2 cell culture incubator at
37 °C. Cells were split 3 to 4 times prior to transfection and they were
transfected at the cell viability of 90% and 1 million cells/mL cell
density. The transfection reagent used was 293fectin (Invitrogen). Culture
supernatant was harvested 72 hours after transfection.
Transient Transfection of Recombinant Proteins
Cell Culture Protocols for Various Cell Lines
Crystallization of Lipid-Binding Proteins
Cell Culture Protocols for HEK293-F, HEK293-T, SCC47, and U87MG
HEK 293-T cells [American Type Culture Collection (ATCC), Manassas, VA, USA] and head and neck SCC4738 (link) cells (kindly supplied by Reidar Grénman, Department of Otorhinolaryngology—Head & Neck Surgery, Turku University, Finland) were grown in Dulbecco’s modified Eagle’s medium (Biological Industries, Beit Haemek, Israel). Glioblastoma U87MG cells (ATCC) were grown in MEM medium (Biological Industries). These two media were supplemented with 10% FBS, 1%
Cultivation of Mammalian Cell Lines for Viral Assays
Detailed Protocol for Protein Expression and Purification
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