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9 protocols using sodium sulfite

1

Enzymatic Assay of L-DOPA Synthesis

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l‐DOPA standard and pyruvate sodium were purchased from Aladdin BioChem Technology (Shanghai, China). Catechol, ammonium acetate, sodium sulfite, and ethylenediamine tetraacetic acid disodium salt were purchased from Sinopharm Chemical Reagent (Shanghai, China). The cozyme pyridoxal 5′‐phosphate (PLP) was purchased from Bide Pharmatech (Shanghai, China). Medium components such as tryptone and yeast extract were purchased Oxoid (Basingstoke, England). Antibiotic kanamycin and isopropyl 1‐β‐d‐thiogalactopyranoside (IPTG) were bought from Sangon Biotech (Shanghai, China). All other chemicals were analytic grade and purchased from commercial sources.
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2

Synthesis of Plasmonic Nanostructures

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Bismuth(III) nitrate pentahydrate (Bi(NO3)3·5H2O), potassium bromide (KBr), titanium(IV) oxide (TiO2), tetrachloroauric(III) acid tetrahydrate (HAuCl4·4H2O), sulfuric acid (H2SO4), and sodium sulfite (Na2SO3) were of analytical grade and supplied by Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). An ultrapure water purification system (Eco‐S15Q, Hitech Instruments Co. Ltd, Shanghai, China) was used to produce 18.2 MΩ cm−1 deionized water (DI water) in all the experiments.
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3

Biologically Synthesized Selenium Nanoparticles

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Sodium SeO32–, sodium hydroxide (NaOH), dipotassium phosphate (K2HPO4), monopotassium phosphate (KH2PO4), potassium persulfate (K2S2O8), sodium sulfite (Na2SO3), n-caprylic alcohol, acetic acid, and hydrochloric acid were obtained from Sinopharm (Shanghai, China). SeNPs were synthesized and purified using a recently described method (Shakibaie et al., 2010 (link)). In brief, the SeNPs were biologically reduced from SeO32– by microorganisms. The reduction product was washed and disrupted in liquid nitrogen. The suspension was then liquid–liquid extracted by n-caprylic alcohol. The purified SeNPs were collected from water phase. More details of synthesis purification and SEM observation were shown in Supplementary Text 1.
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4

Analytical Procedures for DMP Characterization

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Analytical-grade
DMP, hydrogen peroxide (30% w/w), magnesium chloride, sodium hydroxide,
sodium bicarbonate, potassium chloride, sodium nitrate, sodium sulfite,
calcium chloride, sulfuric acid, sodium sulfate, hydrochloric acid,
acetic acid, sodium acetate, hydroxylamine hydrochloride, and ferrous
ammonium sulfate were purchased from Sinopharm Chemical Reagents (Shanghai,
China). Solid potassium ferrate (K2FeVIO4) and potassium bromide (spectrography) were purchased from
MACKLIN Reagent (Shanghai, China). Methanol and acetonitrile (HPLC
grade) were purchased from Merck (Darmstadt, Germany). Methyl tert-butyl ether (GC grade), the GC derivatization reagent,
hexamethyldisilazane, and trimethylchlorosilane were purchased from
Aladdin Reagent (Shanghai, China). Ultrapure water obtained from a
Milli-Q water purification system was used in the experiment. The
DMP stock solution (100 mg·L–1) was prepared
in ultrapure water.
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5

Synthesis of Diquat-Functionalized Silica Particles

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Sodium sulfite (Na2SO3, 97%), sodium bisulfite (NaHSO3, 99%), and cetyltrimethylammonium bromide (CTAB, 99%) were obtained from Sinopharm Chemical Reagent Co, Ltd. (Beijing, China). Diquat dibromide (DQ, 41.5%) was purchased from Shandong LvFeng Pesticide Co. Ltd. (Qingzhou, Shandong province, China). 20% DQ aqueous solution was obtained from Shandong Luba Chemical Co., Ltd. (Jinan, China). Tetraethyl orthosilicate (TEOS) with the purity of 99% was obtained from Fluorochem Ltd. (Hadfield, UK). 3-Glycidoxypropyltrimethoxysilane (GPTMS, 97%) was purchased from J&K Scientific Ltd. (Beijing, China). All other chemicals were commercially available and used as received. Deionized water used for reactions and the treatment process were attained from a Milli-Q water system (Millipore Corporation, Bedford, MA, USA).
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6

Synthesis of Bismuth-Based Thin Films

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Fuorine-doped tin oxide (FTO) coated glass substrates (~30 Ω) were purchased from Pilkington. Bismuth nitrate pentahydrate (Bi(NO3)3·5H2O, >98%), molybdenum diacetylacetonate dioxide (MoO2(acac)2), tri-block copolymer Pluronic® F-108, ethylene glycol (EG), tungsten(VI) oxide nanopowders (WO3, ~100 nm), and Lanthanum(III) nitrate hydrate (La(NO3)3, 99.9%) were obtained from Sigma-Aldrich and used as received. Vanadium acetylacetone oxygen (VO(acac)2, >99%) and glacial acetic acid (>99.7%) were purchased from Acros Organics and Fisher Scientific, respectively. Potassium phosphate monobasic (KH2PO4, >98%), sodium sulfite (Na2SO3, >97%), barium dichloride (BaCl2, >99.5%), tin(II) chloride (SnCl2, >98%), hydrogen peroxide aqueous solution (H2O2), ammonium hydroxide aqueous solution (NH4OH), and sodium hydroxide (NaOH) were purchased from Sinopharm Chemical Reagent Co., Ltd. Au plate was obtained from Zhongnuo Advanced Material (Beijing) Co., Ltd. China. Deionized water was used with a resistivity of 18.25 MΩ·cm.
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7

Macroporous PS-co-DVB Resin Synthesis

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Macroporous PS-co-DVB resin was supplied by Jinkai Resin Chemical Co., Ltd (Jiangsu, China), it had cross-linking degree of 6%, total pore volume (Vtotal) of 0.039 cm3 g−1, average pore size (DP) of 24.3 nm and BET surface area (SBET) of 19.5 m2 g−1. Anhydrous FeCl3, nitric acid, sulfuric acid, hydrochloric acid, acetic anhydride, sodium sulfite, 1,2-dichloroethane (DCE), DCM, aniline (AN) and p-nitroaniline (PNA) were obtained from Sinopharm Chemical Reagent Co., Ltd. SnCl2·6H2O was supplied from Xilong Scientific Co., Ltd (Shantou, China). Methylene blue (MB) was purchased from Macklin Biochemical Co., Ltd (Shanghai, China).
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8

Antioxidant Activity Evaluation of Green Coffee

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Hydroxyhydroquinone (99%), L-cysteine, 2-furfurylthiol (98%), 1,2dichlorobenzene (99%) and ethanol were purchased from Sigma-Aldrich Co. Ltd (Shanghai, China). Disodium hydrogen phosphate, sodium dihydrogen phosphate, sodium sulfite, iron (Ⅲ) chloride hexahydrate (FeCl3•6H2O), iron (Ⅱ) sulfate monohydrate (FeSO4•H2O), hydrogen peroxide (H2O2, 30%), egg albumin, ethylenediaminetetraacetic acid (EDTA, disodium salt), ethyl acetate, ascorbic acid, methionine and glutathione (99%) were purchased from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). Millipore water was made by Ultrapure Water Maker Type 1 (Weston, USA). Green Robusta coffee beans (sun drying, crop years 2016/2017) from Vietnam was purchased from Royal Coffee Co. Ltd (Shanghai, China).
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9

Purification and Analysis of Enzymes

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NaCl, yeast extract, Tryptone, agar, imidazole, d‐glucose, Nicotinamide adenine dinucleotide (NAD+), 3,5‐dinitrosalicylic acid (DNS), sodium hydroxide, potassium sodium tartrate, phenol, sodium sulfite, and sodium nitrate hexahydrate were provided by Sinopharm (Shanghai, China). Kanamycin, isopropyl‐β‐d‐thiogalactoside (IPTG), and dopamine hydrochloride were all purchased from Sigma (St. Louis, MO, USA). The Ni‐NTA resin was supplied by Nano‐Micro Co. Ltd (Suzhou, China). Unless otherwise stated, all chemicals and reagents were commercial analytical‐ or biological‐grade.
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