Anti tubulin sc 23948

Immunoblotting was performed as we have previously described [50 (link)]. Anti-PPP1R1A (ab40877) was purchased from Abcam Inc. (Cambridge, MA, USA). Anti-tubulin (sc-23948) was from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Cell cycle regulation antibody sampler kit (9932) and Rb antibody sampler kit (9969) were from Cell Signaling Technology (Danvers, MA, USA). Quantification of band intensity was performed by ImageJ (https://imagej.nih.gov/ij).

Cells were lysed with RIPA lysis buffer (1 × SDS, 1 × phosphatase inhibitor cocktail, 1 × protease inhibitors) to obtain protein samples. Then, the samples were separated with SDS-PAGE and transferred to the PVDF membrane. The blots were visualized by ECL after incubating with the primary antibodies and secondary antibodies. Anti-GAPDH (sc-47724; 1:500), anti-β-actin (sc-47778; 1:500) and anti-Tubulin (sc-23948; 1:500) were purchased from Santa Cruz Biotechnology, while anti-Flag was purchased from MBL (M185-3L, Tokyo, Japan; 1:1000). Antibodies against NAT10 (ab194297; 1:1000), LANA (ab4103; 1:1000), IFI16 (ab169788; 1:1000) and ac⁴C (ab252215; 1:1000) were from Abcam. Anti-pro Caspase-1+p10+p12 (ab179515; 1:1000) was also obtained from Abcam. IL-1β (3A6) mouse mAb (12242; 1:1000) and cleaved Caspase-1 (Asp296) (E2G2I) rabbit mAb (89332; 1:1000) were from Cell Signaling Technology. The monoclonal rabbit anti-vIL-6 antibody (1:500) was kindly provided by Dr. Robert Yarchoan from the Center for Cancer Research, National Cancer Institute (Bethesda, Maryland, USA)^{53 (link),54 (link)}, and polyclonal rabbit anti-vIRF1 antibody (1:300) was from Dr. Gary Hayward from Viral Oncology Program, The Johns Hopkins School of Medicine (Baltimore, Maryland, USA)^{55 (link)–58 (link)}.

Anti-SETD2 (LS-B12660-50) was from LSBIO (Seattle, WA); anti-H3K36me1 (ab9048), anti-H3K36me2 (ab9049), and H3K36me3 (ab9050) were from Abcam (Cambridge, UK); rabbit-anti-Perilipin A/B (P1873) was from Sigma; rabbit-anti-Collagen I (600-400-103) was from Rockland (PA, USA); anti-Lbp (11836-1AP) and donkey-anti-OPN (AF808) were from R&D Systems (Minneapolis, MN); anit-Osx (sc-133871) and anti-tubulin (sc-23948) were from Santa Cruz Biotechnology (Texas, USA); donkey-anti-goat cy3 (705-165-147) was from Jackson ImmunoResearch (PA, USA); and donkey-anti-rabbit Alexa Fluor 488 (A21206) was from Molecular Probes.