In vitro toxicology assay kit sulforhodamine b

Cell growth was measured with the In vitro Toxicology Assay kit (Sulforhodamine B, Sigma Aldrich, Saint Quentin Fallavier, France) according to manufacturer’s instructions. Cells were plated at a density of 2000 cells per well in 96-well plates in triplicates. Absorbance was measured at 565 nm using the CLARIOstar multiplate reader (BMG Labtech, Champigny-sur-Marne, France) at indicated time points.

Cells were plated at a density of 0.03 × 10⁵ cells/well in a 96-well plate 24 h before treating cells with the desired drugs. Cells were fixed with 10% trichloroacetic acid (TCA) for 1 h, and dried overnight at room temperature followed by staining with SRB for 15 min, washed twice with 1% acetic acid, and air dried for at least 1 h. The incorporated SRB staining was dissolved in 10 mM Tris pH 8.8 solution and then plates were analyzed using a calorimetric microplate reader (Thermo Labsystem Multiskan Ascent) (Akribis Scientific, UK) at a wavelength of 540 nm and 690 nm, according to In Vitro Toxicology Assay Kit Sulforhodamine B based protocol (Sigma, UK).