AD293 cells (Agilent, RRID: CVCL_9804) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Lonza) supplemented with 10% fetal bovine serum (FBS, Gibco), 4mM L-glutamine, 4 mM sodium pyruvate (Lonza) and 100 U/ml penicillin, 100 µg/ml streptomycin and 0.25 µg/ml amphotericin B (Lonza) at 37 °C and 5% CO2. Human lung epithelial cells (A549, kind gift from Prof. Yoshiharu Matsuura, Osaka University, Japan, RRID: CVCL_0023) were grown in DMEM (Sigma-Aldrich) supplemented with 10% fetal bovine serum, 4 mM L-glutamine and 100 U/ml penicillin, 100 µg/ml streptomycin and 0.25 µg/ml amphotericin B at 37 °C and 5% CO2. Escherichia coli BJ5183 cells (Agilent) and E. coli DH5⍺ cells were cultivated in Luria–Bertani medium (1% tryptone, 0.5% yeast extract, 1% NaCl) containing suitable antibiotics (100 µg/ml ampicillin for E. coli BJ5183; 50 µg/ml kanamycin for E. coli DH5⍺-pShuttle-CMV and E. coli DH5⍺-pAdeasy_S).
Kanamycin
Manufactured by Agilent Technologies
Kanamycin is a broad-spectrum antibiotic used in molecular biology and microbiology laboratories. It is an aminoglycoside antibiotic that inhibits bacterial protein synthesis by binding to the 30S subunit of the bacterial ribosome. Kanamycin is commonly used as a selective agent in bacterial transformation and cloning experiments.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions)
Puromycin, by Thermo Fisher Scientific (2 mentions)
Hep3b cells, by American Type Culture Collection (2 mentions)
Expi293f cells, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by American Type Culture Collection (2 mentions)
Expi293 expression media, by Thermo Fisher Scientific (2 mentions)
Bl21 gold de3 cells, by Agilent Technologies (2 mentions)
Hygromycin b, by Thermo Fisher Scientific (2 mentions)
Ad293 cells, by Agilent Technologies (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Amphotericin b, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Lonza (1 mentions)
Amphotericin b, by Lonza (1 mentions)
Penicillin, by Lonza (1 mentions)
Streptomycin, by Lonza (1 mentions)
Sodium pyruvate, by Lonza (1 mentions)
3 protocols using kanamycin
1
Cell Culture of Diverse Cell Lines
2
Cell Culture Conditions for Protein Expression
3
Cell Culture Conditions for Protein Expression
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BL21-Gold(DE3) cells (Agilent Technologies) transformed with recombinant pET-28a(+) or pET-29b(+) plasmids were grown in LB media supplemented with 100 μg/mL kanamycin at 37°C. Expi293F cells (Thermo Fisher Scientific) were maintained in a humidified incubator with 8% CO2 at 37°C with Expi293 expression media (Thermo Fisher Scientific). L6 cells stably co-expressing FGFR1c with KLB (L6R1cKLB) or FGFR4 with KLB (L6R4KLB) were maintained in a humidified incubator with 5% CO2 at 37°C with Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific), 100 U/mL Penicillin-Streptomycin (Thermo Fisher Scientific), 0.1 mg/mL hygromycin B (Thermo Fisher Scientific), and 1 μg/mL puromycin (Thermo Fisher Scientific). L6 cells stably expressing FGFR1b (L6R1b) were maintained in DMEM supplemented with 10% FBS, 100 U/mL Penicillin-Streptomycin, and 1 μg/mL puromycin. HEP3B cells (ATCC) were maintained in a humidified incubator with 5% CO2 at 37°C with Eagle’s Minimum Essential Medium (EMEM, ATCC) supplemented with 10% FBS and 100 U/mL Penicillin-Streptomycin.
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