Anti cd80 pe 16 10a1

The specific surface markers of imDCs and mDCs were characterized by flow cytometry (Cytoflex, Beckman Coulter; Supplementary Figure S2). The upregulated expression levels of co-stimulatory molecules, CD86, CD80, and CD40, antigen-presenting molecule MHC class II (I-A/I-E), and the chemokine receptor CCR7 were evaluated as DC maturation markers, and CD11c and CD11b were analyzed as dendritic cell markers. The following antibodies were used in flow cytometry experiments: anti-CD86-FITC (GL1, 1:200), anti-CD40-PE (1C10, 1:200), anti-MHC class II (I-A/I-E)-FITC (M5/114.15.2, 1:200), and anti-CCR7-PE (4B12, 1:200) were purchased from Thermo Fisher Scientific, and anti-CD11b-APC (M1/70, 1:200), anti-CD11c-APC (HL3, 1:200), and anti-CD80-PE (16-10A1, 1:200) were purchased from BD Biosciences. The acquired data were analyzed using the FlowJo software (BD).

The following antibodies were purchased from BD Biosciences: anti-CD4-V450 (RM4-5), anti-CD45R-V450 (RA3-6B2), anti-CD45R-APC-Cy7 (RA3-6B2), anti-CD45R-A647 (RA3-6B2), anti-CD138-BV421 (281-2), anti-CD19-BV711 (1D3), anti-CD95-PE-Cy7 (Jo2), anti-Bcl6-A647 (K112-91), anti-IgG1-APC (A85-1), anti-CD62L-A700 (MEL-14), anti-CD35-biotin (8C12), anti-CD49D (R1-2), anti-CD62P (RB40.34), anti-IgM-APC (II/41), and anti-CD80-PE (16-10A1). The following antibodies were purchased from BioLegend: anti-CD4-A647 (RM4-5), anti-CD3e-FITC (145-2C11), and anti-rabbit DyLight 649 (Poly4064). The following antibodies were purchased from eBioscience: anti-CD4-PE-Cy5 (RM4-5), anti-PNAd-A488 (MECA-79), anti-CD38-PE (90), anti-IgM-PE-Cy5 (II/41), anti-IgD-APC (11-26c), anti-IgD eF450 (11-26c), anti-CD279-Biotin (RMP1-30), anti-CD273-Biotin (TY25), anti-F4/80-Biotin (BM8), Streptavidin-APC-eF780, and Streptavidin-APC. Anti-Ki-67 (SP6) unconjugated was purchased from Thermo Scientific. FluoroMyelin Red for myelin staining was purchased from Invitrogen.