Log In Sign up
The largest database of trusted experimental protocols

Mouse tgf β1 duoset elisa

Manufactured by R&D Systems
Visit Supplier
Sourced in United States

The Mouse TGF-β1 DuoSet ELISA is a laboratory tool used to measure and quantify the levels of transforming growth factor beta 1 (TGF-β1) in mouse biological samples. It is a sandwich enzyme-linked immunosorbent assay (ELISA) that utilizes specific antibodies to capture and detect the target analyte.

Automatically generated - may contain errors

Lab products found in correlation

Fortessa 2, by BD (2 mentions) Cytometric bead array for th1 th2 th17 cytokines, by BD (2 mentions) Sircol assay kit, by Biocolor (2 mentions) Complete protease inhibitor cocktail, by Merck Group (1 mentions) Dc protein assay, by Bio-Rad (1 mentions) Mouse vegf duoset elisa, by R&D Systems (1 mentions) Human mouse total cox 2 duoset ic elisa, by R&D Systems (1 mentions) Mouse il 1 alpha il 1f1 duoset elisa, by R&D Systems (1 mentions) Mouse il 12 p70 duoset elisa, by R&D Systems (1 mentions) Reagent diluent concentrate 2, by R&D Systems (1 mentions) Human duoset elisa kit, by R&D Systems (1 mentions) Duoset elisa ancillary reagent kit 1, by R&D Systems (1 mentions) Sample activation kit 1, by R&D Systems (1 mentions) Image lab software, by Bio-Rad (1 mentions) Mouse cytokine array c1, by RayBiotech (1 mentions) Human tgf β quantikine elisa kit, by R&D Systems (1 mentions) Human il 6 elisaready set go kit, by Thermo Fisher Scientific (1 mentions) Human tgf β1 duoset elisa, by R&D Systems (1 mentions) Human il 1β elisa ready set go kit, by Thermo Fisher Scientific (1 mentions) Anti hsp90 antibody, by Abcam (1 mentions)

Spelling variants of this product

TGF-β1 (1036 citations) DuoSet ELISA (635 citations) ELISAs (144 citations) DuoSets (49 citations) TGF-β1 DuoSet ELISA (5 citations) Mouse TGF-β1 (5 citations) DuoSet Mouse (2 citations)

12 protocols using mouse tgf β1 duoset elisa

1

Autocrine TGF-β induction in ex vivo DCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ex vivo-isolated DCs were cultured in serum-free media for 60 h. Cell-free supernatants were assayed for TGF-β by ELISA using the mouse TGF-β1 DuoSet ELISA (R & D systems). For autocrine induction of TGF-β, ex vivo-isolated DCs were stimulated with or without TGF-β (0.5 ng/mL) in serum-free media for 12 h, extensively washed, and cultured for another 48 h. Cell-free supernatants were assayed for TGF-β by ELISA.
Garo L.P., Ajay A.K., Fujiwara M., Beynon V., Kuhn C., Gabriely G., Sadhukan S., Raheja R., Rubino S., Weiner H.L, & Murugaiyan G. (2019). Smad7 Controls Immunoregulatory PDL2/1-PD1 Signaling in Intestinal Inflammation and Autoimmunity. Cell reports, 28(13), 3353-3366.e5.
+ Open protocol
+ Expand
2

Measuring Cytokine Profiles in ILC1 Co-Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols
TGFβ-1 concentration in supernatant from 4day ILC1 co-cultures and SIO-only controls was measured using the Mouse TGFβ-1 DuoSet ELISA (R&D Systems) with modified manufacturer’s instructions, whereby 100µl supernatant was incubated with the capture antibody overnight on a shaker at 4°C, not for 2h at RT. Optical density was measured in a plate reader (BioRAD) at 450nm, with correction at 540nm. Concentrations were obtained based on a regression equation (multimember regression, order 3) from the standard curve values (calculated in Microsoft Excel, version 16.16.20).
Cytometric Bead Array for Th1/Th2/Th17 cytokines was obtained from BD biosciences and performed on 10µl supernatant after 4day co-culture following the manufacturers’ instructions, using a BD Fortessa2, and analyzed following the manufacturers’ template based on a standard curve for each cytokine.
Jowett G.M., Norman M.D., Yu T.T., Arévalo P.R., Hoogland D., Lust S.T., Read E., Hamrud E., Walters N.J., Niazi U., Chung M.W., Marciano D., Omer O.S., Zabinski T., Danovi D., Lord G.M., Hilborn J., Evans N.D., Dreiss C.A., Bozec L., Oommen O.P., Lorenz C.D., da Silva R.M., Neves J.F, & Gentleman E. (2020). ILC1 drive intestinal epithelial and matrix remodelling. Nature materials, 20(2), 250-259.
+ Open protocol
+ Expand
3

Quantification of Tumor Cytokine Profiles

Check if the same lab product or an alternative is used in the 5 most similar protocols
The analysis of cytokine expression in tumors of the treatment groups at the protein level was done using different ELISA kits. Mouse Th1/Th2 Uncoated ELISA (Invitrogen) was used to determine the protein levels of IFN-γ, IL-2 and IL-4. IL-1α, IL-12, TGF-β, PDGF-BB, VEGF and COX-2 were measured with the Mouse IL-1 alpha/IL-1F1 DuoSet ELISA, Mouse IL-12 p70 DuoSet ELISA, Mouse TGF-β1 DuoSet ELISA, Mouse/Rat PDGF-BB DuoSet ELISA, Mouse VEGF DuoSet ELISA and Human/Mouse Total COX-2 DuoSet IC ELISA, respectively (all from R&D Systems). TARC was determined with the Mouse CCL17/TARC Quantikine ELISA Kit (R&D Systems). For protein extraction, various sections (of 50 μm thickness) from each tumor were collected and protein lysates were prepared using RIPA buffer (20 mM Tris–HCl (pH 7.2), 150 mM NaCl, 2% (w/v) NP-40, 0.1% (w/v) SDS, 0.5% (w/v) sodium deoxycholate) and cOmplete™ Protease Inhibitor Cocktail (Sigma-Aldrich) [28] (link). The total protein concentration of each tumor sample was determined using the DC protein assay (Bio-Rad Laboratories GmbH). Equal amounts of total protein (50 μg) were diluted with ELISA-Buffer (ELISA/ELISPOT Diluent, Invitrogen), or Reagent Diluent Concentrate 2 (R&D Systems), respectively. Cytokine amounts were determined according to the manufacturers' instructions.
Fiegle E., Doleschel D., Koletnik S., Rix A., Weiskirchen R., Borkham-Kamphorst E., Kiessling F, & Lederle W. (2019). Dual CTLA-4 and PD-L1 Blockade Inhibits Tumor Growth and Liver Metastasis in a Highly Aggressive Orthotopic Mouse Model of Colon Cancer. Neoplasia (New York, N.Y.), 21(9), 932-944.
+ Open protocol
+ Expand
4

Autocrine TGF-β induction in ex vivo DCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ex vivo-isolated DCs were cultured in serum-free media for 60 h. Cell-free supernatants were assayed for TGF-β by ELISA using the mouse TGF-β1 DuoSet ELISA (R & D systems). For autocrine induction of TGF-β, ex vivo-isolated DCs were stimulated with or without TGF-β (0.5 ng/mL) in serum-free media for 12 h, extensively washed, and cultured for another 48 h. Cell-free supernatants were assayed for TGF-β by ELISA.
Garo L.P., Ajay A.K., Fujiwara M., Beynon V., Kuhn C., Gabriely G., Sadhukan S., Raheja R., Rubino S., Weiner H.L, & Murugaiyan G. (2019). Smad7 Controls Immunoregulatory PDL2/1-PD1 Signaling in Intestinal Inflammation and Autoimmunity. Cell reports, 28(13), 3353-3366.e5.
+ Open protocol
+ Expand
5

Measuring Cytokine Profiles in ILC1 Co-Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols
TGFβ-1 concentration in supernatant from 4day ILC1 co-cultures and SIO-only controls was measured using the Mouse TGFβ-1 DuoSet ELISA (R&D Systems) with modified manufacturer’s instructions, whereby 100µl supernatant was incubated with the capture antibody overnight on a shaker at 4°C, not for 2h at RT. Optical density was measured in a plate reader (BioRAD) at 450nm, with correction at 540nm. Concentrations were obtained based on a regression equation (multimember regression, order 3) from the standard curve values (calculated in Microsoft Excel, version 16.16.20).
Cytometric Bead Array for Th1/Th2/Th17 cytokines was obtained from BD biosciences and performed on 10µl supernatant after 4day co-culture following the manufacturers’ instructions, using a BD Fortessa2, and analyzed following the manufacturers’ template based on a standard curve for each cytokine.
Jowett G.M., Norman M.D., Yu T.T., Arévalo P.R., Hoogland D., Lust S.T., Read E., Hamrud E., Walters N.J., Niazi U., Chung M.W., Marciano D., Omer O.S., Zabinski T., Danovi D., Lord G.M., Hilborn J., Evans N.D., Dreiss C.A., Bozec L., Oommen O.P., Lorenz C.D., da Silva R.M., Neves J.F, & Gentleman E. (2020). ILC1 drive intestinal epithelial and matrix remodelling. Nature materials, 20(2), 250-259.
+ Open protocol
+ Expand
6

Quantification of TGFβ Secretion in Murine Pancreatic Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols
The amount of TGFβ secreted by mouse pancreatic tumor cells was quantified using the Mouse TGFβ1 DuoSet ELISA (#DY1679–05, R&D systems), complemented with Sample Activation Kit 1 (#DY010, R&D systems) and DuoSet ELISA Ancillary Reagent Kit 1 (#DY007, R&D systems) according to the manufacturer’s instructions. Conditioned media for TGFβ secretion quantification was collected from KPC and KPCN tumor cells growing in DMEM supplemented with 1% v/v FBS and 2mM L-glutamine with 100μg/ml Penicillin/Streptomycin for 48 hours. Protein concentration of lysates, prepared from remaining cell pellets, was used for data normalization. Human TGFβ1 in serum samples from patients was measured by human DuoSet ELISA kit (#DY240, R&D systems).
Gabitova-Cornell L., Surumbayeva A., Peri S., Franco-Barraza J., Restifo D., Weitz N., Ogier C., Goldman A.R., Hartman T.R., Francescone R., Tan Y.F., Nicolas E., Shah N., Handorf E.A., Cai K.Q., O’Reilly A.M., Sloma I., Chiaverelli R., Moffitt R.A., Khazak V., Fang C.Y., Golemis E.A., Cukierman E, & Astsaturov I. (2020). Cholesterol pathway inhibition induces TGFβ signaling to promote basal differentiation in pancreatic cancer.. Cancer cell, 38(4), 567-583.e11.
+ Open protocol
+ Expand
7

Quantifying Collagen and TGF-β in BALF

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total acid soluble collagen content in cell free BALF was determined using the Sircol assay kit (Biocolor, Ltd; Carrickfergus, UK) according to the manufacturer’s instructions. TGFβ concentration in the cell free BALF was calculated using Mouse TGFβ 1 DuoSet Elisa (R&D Systems Cat DY1679–05) according to the manufacturer’s instructions.
Rodriguez L.R., Tang S.Y., Barboza W.R., Murthy A., Tomer Y., Cai T.Q., Iyer S., Chavez K., Das U.S., Ghosh S., Dimopoulos T., Babu A., Connelly C., FitzGerald G.A, & Beers M.F. (2023). Disruption of Prostaglandin F2α Receptor Signaling Attenuates Fibrotic Remodeling and Alters Fibroblast Population Dynamics in A Preclinical Murine Model of Idiopathic Pulmonary Fibrosis. bioRxiv.
+ Open protocol
+ Expand
8

Quantifying BALF Collagen and TGF-β

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total acid soluble collagen content in cell-free BALF was determined using the Sircol assay kit (Biocolor) according to the manufacturer’s instructions. Total TGF-β concentration (latent and active) in the cell-free BALF was calculated using Mouse TGF-β1 DuoSet Elisa (R&D Systems, DY1679-05) according to the manufacturer’s instructions.
Rodriguez L.R., Tang S.Y., Roque Barboza W., Murthy A., Tomer Y., Cai T.Q., Iyer S., Chavez K., Das U.S., Ghosh S., Cooper C.H., Dimopoulos T.T., Babu A., Connelly C., FitzGerald G.A, & Beers M.F. (2023). PGF2α signaling drives fibrotic remodeling and fibroblast population dynamics in mice. JCI Insight, 8(24), e172977.
+ Open protocol
+ Expand
9

Cytokine and Chemokine Profiling in NTHi-Infected Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cytokine and chemokine levels were measured in middle ear fluids collected from NTHi-infected Junbo mice (n = 3) by using a mouse cytokine antibody array (mouse cytokine array C1; RayBiotech, Inc.) according to the manufacturer’s protocol. The following cytokines and chemokines were analyzed: G-CSF, GM-CSF, and IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40/p70, IL-12p70, IL-13, and IL-17, as well as IFN-γ, monocyte chemoattractant proteins 1 and 5 (MCP-1 and MCP-5), RANTES (T cell expressed and secreted), stem cell factor (SCF), soluble TNF receptor type I (sTNFRI), TNF-α, TPO, and VEGF. The signal intensities were quantified using the Image Lab software (Bio-Rad). Changes in middle ear fluid TGF-β levels were analyzed by Mouse TGF-β 1 DuoSet ELISA (R&D Systems) according to the manufacturer’s instruction. The color change was measured at 450 nm on an Epoch BioTek plate reader, and the TGF-β concentration in middle ear fluid was calculated by using a standard curve.
Vikhe P.P., Purnell T., Brown S.D, & Hood D.W. (2019). Cellular Immune Response against Nontypeable Haemophilus influenzae Infecting the Preinflamed Middle Ear of the Junbo Mouse. Infection and Immunity, 87(12), e00689-19.
+ Open protocol
+ Expand
10

TGFβ1 Measurement in Mammosphere Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols
TGFβ1 was measured in the conditioned medium of E‐cadherin‐RFP/Py2T cells using human TGFβ Quantikine ELISA kit and mouse TGFβ1 Duoset ELISA (R&D Systems Inc., Minneapolis, MN, USA, Cat# DB100B and Cat# DY1679‐05, respectively), according to the manufacturer’s instructions. Conditioned medium was collected from approximately 80 3D mammospheres and after 1 : 10 dilution was analyzed by ELISA. For human TGFβ1 ELISA, stem cell or 2D cell culture media were used as negative controls. For mouse TGFβ1 ELISA, 2 ng·mL−1 human TGFβ1 was used as negative control.
Tsubakihara Y., Ohata Y., Okita Y., Younis S., Eriksson J., Sellin M.E., Ren J., ten Dijke P., Miyazono K., Hikita A., Imamura T., Kato M., Heldin C, & Moustakas A. (2022). TGFβ selects for pro‐stemness over pro‐invasive phenotypes during cancer cell epithelial–mesenchymal transition. Molecular Oncology, 16(12), 2330-2354.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!