Rapamycin (Rapa) was purchased from Tocris Bioscience (Bristol, UK). Cycloheximide (CHX), tunicamycin (TUN), 6-diazo-5-oxo-L-norleucine (DON), reduced L-glutathione (GSH), reduced glutathione ethyl ester (GSHee) were from Sigma-Aldrich (St. Louis, MO, USA). OU749, buthionine sulfoximine (BSO), and ferrostatin-1 (Fer-1) were from Cayman Chemicals (Ann Arbor, MI, USA). Antibodies specific for p70S6 kinase, phospho-p70S6 kinase (Thr 389), S6 ribosomal protein, phospho-S6 ribosomal protein (Ser 235/236), eIF2α, phospho-eIF2α (Ser 51), PERK, Bip and horseradish peroxidase (HRP)-linked secondary antibodies were from Cell Signaling Technology (Danvers, MA, USA). Antibody specific for phospho-GCN2 (Thr 899) was from Abcam (Cambridge, UK). ATF4 antibody was from Santa Cruz Biotechnology (Dallas, TX, USA).
Ferrostatin 1 fer 1
Manufactured by Cayman Chemical
Sourced in United States
Ferrostatin-1 (Fer-1) is a chemical compound used in research applications. It functions as a potent and selective inhibitor of ferroptosis, a form of regulated cell death. Fer-1 is utilized in experimental studies to investigate cellular processes and mechanisms related to ferroptosis.
Automatically generated - may contain errors
Lab products found in correlation
Reduced glutathione ethyl ester, by Merck Group (1 mentions)
Horseradish peroxidase hrp linked secondary antibody, by Cell Signaling Technology (1 mentions)
Eif2α, by Cell Signaling Technology (1 mentions)
L glutathione reduced, by Merck Group (1 mentions)
Ou749, by Cayman Chemical (1 mentions)
Buthionine sulfoximine bso, by Cayman Chemical (1 mentions)
6 diazo 5 oxo l norleucine, by Merck Group (1 mentions)
Phospho s6 ribosomal protein ser235 236, by Cell Signaling Technology (1 mentions)
Erastin, by Selleck Chemicals (1 mentions)
Dako envision system peroxidase kit, by Agilent Technologies (1 mentions)
Mouse monoclonal antibodies against β actin, by Merck Group (1 mentions)
3 protocols using ferrostatin 1 fer 1
1
Modulation of mTOR and eIF2α Pathways
2
Cytotoxicity Evaluation of Baicalein
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Baicalein (purity ≥ 98%) was purchased from Aladdin Reagent Co., Ltd. (Shanghai, China). Phenylmethylsulfonyl fluoride (PDMSF) was purchased from Sigma (St. Louis, MO, USA). CCl4 was purchased from Kaixing Chemical Industry Co., Ltd. (Tianjin, China). Ferrostatin-1 (Fer-1) was purchased from Cayman Chemicals (Ann Arbor, MI, USA). Erastin was purchased from Selleck Chemicals (Shanghai, China). All other reagents were of the highest analytical reagent grade available.
3
Antibody and Reagent Procurement for Cell Analysis
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PQ dichloride (1,1-dimethyl-4,4-dipyridinium dichloride) and MTX were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Rabbit antibodies against ACSL4 (FACL4: ab155282) were purchased from Abcam (Cambridge, MA, USA). Mouse monoclonal antibodies against β-actin were purchased from Sigma Aldrich (St. Louis, MO, USA). Rat monoclonal antibodies against mouse Ly6G (clone 1A8) were purchased from Bio X cell (Lebanon, NH, USA). The DAKO Envision+ system peroxidase kit was purchased from DAKO (Carpinteria, CA, USA). Anti-mouse CD11b-FITC (11-0112-82), anti-mouse Ly-6G/6C-PE (12-5931-82), and anti-mouse F4/80-PE-Cy5 (15-4801-82) antibodies were purchased from eBioscience (San Diego, CA, USA). Ferrostatin-1 (Fer-1) was purchased from Cayman Chemical (Ann Arbor, MI, USA).
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