Eagle 4k 4k ccd camera

Manufactured by Thermo Fisher Scientific

Sourced in Netherlands, United States, Germany

The Eagle 4k × 4k CCD camera is a scientific imaging device that captures high-resolution digital images. It has a sensor resolution of 4096 × 4096 pixels, providing a large field of view and detailed image capture. The camera is designed for scientific and industrial applications that require high-quality image data.

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47 protocols using eagle 4k 4k ccd camera

Transmission Electron Microscopy Imaging

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Transmission electron microscopy (TEM) was conducted by NanoImaging Inc. (San Diego, CA, USA). Briefly, samples were diluted to working concentrations of ~0.05 mg/mL using a supplied final fill buffer. Each sample was imaged over a layer of continuous carbon supported by nitrocellulose on a 400-mesh copper grid. Grids were prepared by adding 3 µL of sample, blotting away excess with filter paper, and immediately staining with uranyl formate. TEM was performed using an FEI Tecnai T12 electron microscope operating at 120 keV equipped with an FEI Eagle 4k × 4k CCD camera.

Royal J.M., Simpson C.A., McCormick A.A., Phillips A., Hume S., Morton J., Shepherd J., Oh Y., Swope K., DeBeauchamp J.L., Webby R.J., Cross R.W., Borisevich V., Geisbert T.W., Demarco J.K., Bratcher B., Haydon H, & Pogue G.P. (2021). Development of a SARS-CoV-2 Vaccine Candidate Using Plant-Based Manufacturing and a Tobacco Mosaic Virus-Like Nano-Particle. Vaccines, 9(11), 1347.

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Cryo-EM Imaging of Vesicle Samples

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For the preparation of thin vitrified specimens, a 3-μL drop of the sample was transferred to a glow discharged Quantifoil micromachined Holey Carbon (R 2/2) TEM grid (Quantifoil Micro Tools GmbH, Jena, Germany) and held by the Vitrobot mark IV tweezer (FEI, Eindhoven, The Netherlands). The temperature in the Vitrobot chamber was set at room temperature (25 °C) and the humidity to 100%. Excess of the sample was removed by blotting filter papers and the grid was immediately frozen in liquid ethane and transferred into a Tecnai20 LaB6 electron microscope (FEI, Eindhoven, The Netherlands). The specimen’s temperature was held below −165 °C during the whole procedure to prevent ice formation. An Eagle 4k × 4k CCD camera (FEI, Eindhoven, The Netherlands) was used to record micrographs of the vesicles, which was done in Tif format with a nominal under focus of 3 μm. Vesicle diameter was measured using the ImageJ software.

Rontogianni S., Synadaki E., Li B., Liefaard M.C., Lips E.H., Wesseling J., Wu W, & Altelaar M. (2019). Proteomic profiling of extracellular vesicles allows for human breast cancer subtyping. Communications Biology, 2, 325.

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Structural Analysis of OspA-Ferritin Nanoparticles

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OspA-ferritin nanoparticles were diluted 300-fold in TBS and imaged over a layer of continuous carbon supported by nitrocellulose on a 400-mesh copper grid. The grids were prepared by applying 3 µl of sample suspension to a cleaned grid, blotting away with filter paper, and immediately staining with uranyl formate. Electron microscopy was performed using an FEI Tecnai T12 electron microscope equipped with an FEI Eagle 4 k × 4 K CCD camera. High magnification images were acquired at normal magnification of 67,000 (0.16 nm/pixel). The images were acquired at a nominal underfocus of −1.9 µm to −0.8 µm and electron doses of ~30 e⁻/Å². Individual particles in the 67,000x high magnification images were selected using automated picking protocols^{41 (link)}. A reference-free alignment strategy was used based on the XMIPP processing package^{42 (link)}. Algorithms in this package align the selected particles and sort them into self-similar groups or classes.

Kamp H.D., Swanson K.A., Wei R.R., Dhal P.K., Dharanipragada R., Kern A., Sharma B., Sima R., Hajdusek O., Hu L.T., Wei C.J, & Nabel G.J. (2020). Design of a broadly reactive Lyme disease vaccine. NPJ Vaccines, 5, 33.

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Ultrastructural analysis of chloroplasts in leaves

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Ultrastructural analysis of chloroplasts in 10-day-old primary leaves of WT and W1 plants grown under LL and HL was done as described before by Saeid Nia et al. (2022 (link)). Four leaves were analyzed per condition. Segments from the mid part of primary foliage leaves were dissected and fixed with 1% glutaraldehyde in 200 mM Hepes, pH 7.4. Samples were post-fixed with 1% OsO₄ prepared in 1.5% aqueous potassium ferricyanide, contrasted en-bloc with 2% aqueous uranyl acetate, then dehydrated with a graded ethanol series, followed by 100% acetone, next progressively infiltrated with epon resin and then heat polymerised. Ultrathin 80-nm sections were contrasted with saturated aqueous uranyl acetate and lead citrate, and inspected in a Tecnai G2 Spirit BioTWIN transmission electron microscope (FEI, now Thermo Fisher Scientific) equipped with an Eagle 4k × 4k CCD camera and TIA software (both FEI).

Saeid Nia M., Scholz L., Garibay-Hernández A., Mock H.P., Repnik U., Selinski J., Krupinska K, & Bilger W. (2023). How do barley plants with impaired photosynthetic light acclimation survive under high-light stress?. Planta, 258(4), 71.

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Tomographic Imaging of Plastic Samples

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The plastic sections (200–250 nm) were placed in a Fischione 2020 single-tilt holder (PA, USA), and examined in a Tecnai TF20 transmission electron microscope (FEI, The Netherlands) operated at 200 kV (Yale Center for Cellular and Molecular Imaging, Yale University). The single-tilt series were collected at every 1° over a ± 60° range using a FEI Eagle 4K × 4K CCD camera. Images were aligned using gold particles, the final tomograms were reconstructed using R-weighted algorithm of the IMOD software⁷¹.

Shen H., Giordano F., Wu Y., Chan J., Zhu C., Milosevic I., Wu X., Yao K., Chen B., Baumgart T., Sieburth D, & De Camilli P. (2014). Coupling between endocytosis and sphingosine kinase I recruitment. Nature cell biology, 16(7), 652-662.

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Negative Staining and TEM Analysis of Tau Filaments

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Aliquots (5 μl of 0.5 mg/ml protein solution) of sarkosyl-insoluble P3 fractions or CSA samples were loaded onto freshly glow-discharged 400 mesh carbon coated copper grids (Electron Microscopy Sciences) and adsorbed for ~ 1 min. Next, the grids were sequentially washed with 50 μl each of 0.1 M and 0.01 M ammonium acetate and negatively stained with 2 × 50 μl of freshly filtered 2% uranyl acetate. After drying, the grids were examined with a Tecnai G20 transmission electron microscope (FEI Company) using an acceleration voltage of 200 kV. Electron micrographs were recorded with an Eagle 4 k × 4 k CCD camera (FEI Company). Morphologies of individual tau filaments were classified into "straight filaments", "coiled filaments", and "twisted ribbon-like filaments" as described previously [29 (link)].

Daude N., Kim C., Kang S.G., Eskandari-Sedighi G., Haldiman T., Yang J., Fleck S.C., Gomez-Cardona E., Han Z.Z., Borrego-Ecija S., Wohlgemuth S., Julien O., Wille H., Molina-Porcel L., Gelpi E., Safar J.G, & Westaway D. (2020). Diverse, evolving conformer populations drive distinct phenotypes in frontotemporal lobar degeneration caused by the same MAPT-P301L mutation. Acta Neuropathologica, 139(6), 1045-1070.

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Negative Stain and Cryo-EM Imaging of Liposomes

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For negative stain EM, liposomes were applied for 3 minutes onto a glow discharged carbon-coated 400-Cu mesh grids (Electron Microscopy Sciences, Hatfield, PA). Excess sample was removed and the grids were immediately placed on a droplet of 2% phosphotungstic acid solution (pH 6.9) for 2 minutes. Excess stain was removed and the grids were allowed to dry thoroughly. Grids were examined on a Philips CM100 electron microscope (FEI, Hillsbrough OR) at 80 kV, and images were acquired with a Megaview III charge-coupled device (CCD) camera (Olympus Soft Imaging Solutions Germany). For cryo EM, samples were preserved undiluted in vitrified ice supported by holey carbon films on 400-mesh copper grids. Samples were prepared by applying 3 μl drop of sample suspension to a clean grid blotting away excess with filter paper and immediately proceeded with vitrification in liquid ethane. Grids were stored under liquid nitrogen until transferred to the electron microscope for imaging. Electron microscopy was performed using an FEI Tecnai T12 electron microscope operating at 120keV equipped with an FEI Eagle 4k × 4k CCD camera. Vitreous ice grids were transferred into the electron microscope using a cryostage that maintains the grids at a temperature below −170°C.

Ingale J., Stano A., Guenaga J., Sharma S.K., Nemazee D., Zwick M.B, & Wyatt R.T. (2016). High-density array of well-ordered HIV-1 spikes on synthetic liposomal nanoparticles efficiently activate B cells. Cell reports, 15(9), 1986-1999.

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Structural Analysis of 60S-Sdo1p Complexes

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The 60S subunit (100 nmol/L) was incubated with 20-fold or 10-fold excess of full-length Sdo1p at 30°C for 15 min. The mixture was diluted to a final concentration of 60 nmol/L for the 60S complex in binding buffer. 4-µL aliquots of samples were applied to 300-mesh 2/2 glow-discharged Quantifoil grids (Quantifoil Micro Tools) which were pre-coated with a thin layer of carbon. The grids were blotted and plunged into liquid ethane with an FEI Mark IV Vitrobot operated at 4°C. For the sample of dimeric 60S-Sdo1p complexes (20-fold excess of Sdo1p), data collection was performed with an FEI F20 at 80,000× magnification with a Gatan UltraScan 4000 CCD camera. For the sample of monomeric 60S-Sdo1p complexes, data collection was done with an FEI Titan Krios equipped with an FEI Eagle 4 K × 4 K CCD camera at 75,000× magnification. All the images were recorded under low-dose conditions (~20 e-/Å²) with AutoEMation package (Lei and Frank, 2005 (link)).

Ma C., Yan K., Tan D., Li N., Zhang Y., Yuan Y., Li Z., Dong M.Q., Lei J, & Gao N. (2016). Structural dynamics of the yeast Shwachman-Diamond syndrome protein (Sdo1) on the ribosome and its implication in the 60S subunit maturation. Protein & Cell, 7(3), 187-200.

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Tau Filament Morphology Analysis

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Aliquots (5 μL) of sarkosyl-insoluble P3 fractions were loaded onto freshly glow-discharged 400 mesh carbon coated copper grids (Electron Microscopy Sciences) and adsorbed for ~1 min. Next, the grids were washed with 50 μL each of 0.1 M and 0.01 M ammonium acetate respectively and negatively stained with 2 × 50 μL of freshly filtered 2% uranyl acetate. After drying, the grids were examined with a Tecnai G20 transmission electron microscope (FEI Company) using an acceleration voltage of 200 kV. Electron micrographs were recorded with an Eagle 4 k × 4 k CCD camera (FEI Company). The morphology of individual Tau filaments was readily visible and classified into “straight filaments”, “coiled filaments”, and “twisted ribbon-like filaments”.

Eskandari-Sedighi G., Daude N., Gapeshina H., Sanders D.W., Kamali-Jamil R., Yang J., Shi B., Wille H., Ghetti B., Diamond M.I., Janus C, & Westaway D. (2017). The CNS in inbred transgenic models of 4-repeat Tauopathy develops consistent tau seeding capacity yet focal and diverse patterns of protein deposition. Molecular Neurodegeneration, 12, 72.

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Quantifying Mesophyll Cell Cytoplasm

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Ultra-thin, 80-nm epon sections were transferred onto formvar coated slot grids, and contrasted with saturated aqueous uranyl acetate for 10 min, followed by 0.2% lead citrate for 3 min. Sections were inspected in a Tecnai G2 Spirit BioTWIN transmission electron microscope (FEI, now Thermo Fisher Scientific), operated at 80 kV, and equipped with a LaB6 filament, an Eagle 4 k × 4 k CCD camera and a TIA software (both FEI, now Thermo Fisher Scientific).
The volume fraction of cytoplasm in mesophyll cells was determined by a stereological analysis of electron micrographs. Images were collected at 890 × magnification by systematic uniform random (SUR) sampling. In the Fiji software (Schindelin et al. 2012 (link)), 15 and 30 μm² square test grids were used for point counting to estimate the area of the cytoplasm and of cell profiles, respectively. For each leaf, between 15 and 23 electron micrographs were analysed. The cytoplasm to cell volume fraction was calculated as a ratio between the total count of points over the cytoplasm and the total count of points over the reference area of cells. For each sample group, at least three leaves were analysed to obtain three estimates.

Saeid Nia M., Repnik U., Krupinska K, & Bilger W. (2022). The plastid-nucleus localized DNA-binding protein WHIRLY1 is required for acclimation of barley leaves to high light. Planta, 255(4), 84.

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