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Vetscan 2

Manufactured by Abaxis
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Sourced in United States

The Vetscan 2 is a compact, fully automated, in-house laboratory analyzer designed for veterinary use. It provides rapid and accurate diagnostic results for a wide range of common tests, including chemistry, electrolyte, and immunoassay panels. The Vetscan 2 is intended to assist veterinary professionals in making informed clinical decisions.

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Lab products found in correlation

Preventive care profile disks, by Abaxis (4 mentions) Procyte dx, by IDEXX (4 mentions) Human albumin elisa quantitation set, by Fortis Life Sciences (2 mentions) Fluoview fv1000mpe, by Olympus (1 mentions) Comprehensive diagnostic profile rotor, by Abaxis (1 mentions) Mammalian liver profile, by Abaxis (1 mentions)

19 protocols using vetscan 2

1

Liver Function Monitoring in Rat Transplant

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The tail vein was phlebotomized prior to transplantation, 48–72 hours after transplantation, and at time of sacrifice. Concentration of serum alanine transaminase (ALT) in whole blood was measured using VetScan 2.0 (Abaxis, Union City, CA). The presence of human albumin in the rat serum was evaluated using a Human Albumin ELISA Quantitation Set that was non-cross reactive with rat albumin (Bethyl Laboratories).
Pettinato G., Ramanathan R., Fisher R.A., Mangino M.J., Zhang N, & Wen X. (2016). Scalable Differentiation of Human iPSCs in a Multicellular Spheroid-based 3D Culture into Hepatocyte-like Cells through Direct Wnt/β-catenin Pathway Inhibition. Scientific Reports, 6, 32888.
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2

Liver Function Monitoring Post-Transplant

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The tail vein was phlebotomized prior to transplantation, 48–72 hours after transplantation, and at time of sacrifice. We measured of serum alanine transaminase (ALT) concentration in whole blood using VetScan 2.0 (Abaxis, Union City, CA). Presence of human albumin in the rat serum was then evaluated using a Human Albumin ELISA Quantitation Set that was non-cross reactive with rat albumin (Bethyl Laboratories).
Pettinato G., Lehoux S., Ramanathan R., Salem M.M., He L.X., Muse O., Flaumenhaft R., Thompson M.T., Rouse E.A., Cummings R.D., Wen X, & Fisher R.A. (2019). Generation of fully functional hepatocyte-like organoids from human induced pluripotent stem cells mixed with Endothelial Cells. Scientific Reports, 9, 8920.
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3

Quantification of Liver Lipids and Collagen

Cited 1 time
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Serum ALT levels were measured using a commercially available veterinary scanner (Vet Scan 2, Abaxis). Cholesterol levels were measured using a commercially available assay (Cholesterol/Cholesteryl Ester Quantification Kit II, K623-100, BioVision). Cholesterol concentrations in High Density Lipoprotein (HDL) and Low Density Lipoprotein/Very Low Density Lipoprotein (LDL/VLDL) were measured using a commercially available assay (EnzyChrom™ AF HDL and LDL/VLDL Assay Kit, E2HL-100) by colorimetric procedure. Coherent Anti-stokes Raman Scattering (CARS) and Second Harmonic Generation (SHG) microscopy were performed on label-free liver cryo sections (7 μm) imaged on a two photon confocal microscope FluoView FV1000 MPE (Olympus America, Center Valley, PA) using the CARS and SHG applications as previously described by us26 (link). Images were obtained using XLPlanN 25×/1.05w MP objective lens. For quantification, the areas of the CARS (neutral lipid) or SHG (collagen) signal having intensity above the threshold value were calculated using ImageJ (NIH).
Bandla H., Dasgupta D., Mauer A.S., Nozickova B., Kumar S., Hirsova P., Graham R.P, & Malhi H. (2018). Deletion of endoplasmic reticulum stress-responsive co-chaperone p58IPK protects mice from diet-induced steatohepatitis. Hepatology research : the official journal of the Japan Society of Hepatology, 48(6), 479-494.
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4

Plasma Biochemical Analysis of Liver Function

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Plasma concentration of liver enzymes and bilirubin were determined using a commercial Preventive Care Profile Plus test and Vetscan 2 device (Abaxis, United States), as previously (Igreja Sa et al., 2020 (link)). Analysis of endogenous BA content in plasma, bile, and feces was performed by liquid chromatography coupled with mass spectrometry (LC-MS) as described previously (Dolezelova et al., 2019 (link); Igreja Sa et al., 2020 (link)).
Cristina Igreja Sá I., Tripska K., Alaei Faradonbeh F., Hroch M., Lastuvkova H., Schreiberova J., Kacerovsky M., Pericacho M., Nachtigal P, & Micuda S. (2023). Labetalol and soluble endoglin aggravate bile acid retention in mice with ethinylestradiol-induced cholestasis. Frontiers in Pharmacology, 14, 1116422.
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5

Mouse Blood Analysis Protocol

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Mouse blood samples were collected via cheek bleeds or post mortem from the heart in lithium-heparin coated tubes. Blood samples were analyzed using an Abaxis Vetscan2 instrument and the preventative care profile plus rotor. Complete blood count (CBC) measurements were obtained via whole blood using a scil Vet ABC Instrument. These instruments provided the normal ranges of laboratory values (Tables 1–4). Creatinine kinase and lactase dehydrogenase levels were measured using colorimetric kits purchased from BioAssay systems (cat# D2Dh-100 and ECPK-100).
Haney S.L., Chhonker Y.S., Varney M.L., Talmon G., Murry D.J, & Holstein S.A. (2018). Preclinical Investigation of a Potent Geranylgeranyl Diphosphate Synthase Inhibitor. Investigational new drugs, 36(5), 810-818.
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6

Hematology and Serum Chemistry Analysis

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Hematology was evaluated on EDTA-treated whole-blood using a Procyte DX (IDEXX Laboratories) and serum chemistries were completed on a Vetscan 2 (Abaxis) using Preventive care profile disks (Abaxis). Indicated normal ranges for blood parameters were obtained from published resources 49 ,50 (link).
Hawman D.W., Ahlén G., Appelberg K.S., Meade-White K., Hanley P.W., Scott D., Monteil V., Devignot S., Okumura A., Weber F., Feldmann H., Sällberg M, & Mirazimi A. (2020). A DNA-based vaccine protects against Crimean-Congo hemorrhagic fever virus disease in a Cynomolgus macaque model. Nature microbiology, 6(2), 187-195.
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7

Hematology and Serum Chemistry Analysis

Cited 1 time
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Hematology was evaluated on EDTA-treated whole-blood using a Procyte DX (IDEXX Laboratories) and serum chemistries were completed on a Vetscan 2 (Abaxis) using Preventive care profile disks (Abaxis). Indicated normal ranges for blood parameters were obtained from published resources 49 ,50 (link).
Hawman D.W., Ahlén G., Appelberg K.S., Meade-White K., Hanley P.W., Scott D., Monteil V., Devignot S., Okumura A., Weber F., Feldmann H., Sällberg M, & Mirazimi A. (2020). A DNA-based vaccine protects against Crimean-Congo hemorrhagic fever virus disease in a Cynomolgus macaque model. Nature microbiology, 6(2), 187-195.
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8

Histopathological Evaluation of NAFLD

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We stained 5‐μm sections of formalin‐fixed paraffin‐embedded liver tissues with hematoxylin and eosin using standard techniques; these were used for histologic grading according to the NAFLD activity score (NAS) by a blinded expert pathologist.29 Fibrosis was assessed by sirius red staining of 5‐µm liver sections, as described by us in detail, and also graded in a blinded fashion by an expert pathologist.30 The percentage area of sirius red staining was quantified using National Institutes of Health ImageJ software. Plasma ALT levels were measured using a commercial veterinary chemistry analyzer (VetScan 2; Abaxis).
Li J., Liu H., Mauer A.S., Lucien F., Raiter A., Bandla H., Mounajjed T., Yin Z., Glaser K.J., Yin M, & Malhi H. (2019). Characterization of Cellular Sources and Circulating Levels of Extracellular Vesicles in a Dietary Murine Model of Nonalcoholic Steatohepatitis. Hepatology Communications, 3(9), 1235-1249.
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9

Biochemical Analysis of Bovine Blood

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At the end of the methane measurement trial (38th day), the blood samples (5 ml) were collected from the jugular vein in heparinized tubes. Total protein, albumin, globulin, minerals (Ca, P, Mg) and enzymes (alkaline phosphatase, alanine aminotransferase, glutamyl transferase, and creatine kinase) were analyzed in an automatic blood analyzer (VetScan 2, Abaxis, Germany) following the manufacturer’s instructions.
Malik P.K., Trivedi S., Kolte A.P., Mohapatra A., Bhatta R, & Rahman H. (2022). Effect of an anti-methanogenic supplement on enteric methane emission, fermentation, and whole rumen metagenome in sheep. Frontiers in Microbiology, 13, 1048288.
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10

Blood Cell and Biochemistry Analysis

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Blood samples were collected in tubes containing EDTA where the total cell counts were determined via Idexx ProCyte DX analyzer (Idexx Laboratories, Westbrook, ME). Serum biochemistry was analysed on a Vetscan 2 using Preventive care profile disks (Abaxis, Union City, CA).
Fletcher P., O’Donnell K.L., Doratt B.M., Malherbe D.C., Clancy C.S., Rhoderick J.F., Feldmann F., Hanley P.W., Ksiazek T.G., Geisbert T.W., Messaoudi I, & Marzi A. (2023). Single-dose VSV-based vaccine protects cynomolgus macaques from disease after Taï Forest virus infection. Emerging Microbes & Infections, 12(2), 2239950.
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