Nefa e test

Manufactured by Fujifilm

Sourced in Japan

The NEFA E-test is a laboratory equipment product for the quantitative determination of non-esterified fatty acids (NEFA) in various biological samples. It provides a reliable and accurate method for measuring NEFA levels, which is a key parameter in the assessment of lipid metabolism and related health conditions.

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Lab products found in correlation

Tg e test, by Fujifilm (1 mentions) Dri chem4000, by Fujifilm (1 mentions) Triglyceride e test, by Fujifilm (1 mentions)

3 protocols using nefa e test

Serum Biomarkers for Metabolic Profiling

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Serum levels of free fatty acids (FFA), leptin, TG, total cholesterol (T-CHO), high-density lipoprotein cholesterol (HDL-C), glutamic-oxaloacetic transaminase (GOT), and glutamic-pyruvic transaminase (GPT) were measured using commercially available kits according to the manufacturer’s instructions (FFA: NEFA E-test, Wako Pure Chemical Industries Ltd., Osaka, Japan; leptin: YK050 Rat Leptin ELISA Kit, Yanaihara Institute, Shizuoka, Japan; TG: TG E-test, Wako Pure Chemical Industries Ltd.; T-CHO, HDL-C, GOT, and GPT: Dri-Chem 4000, Fujifilm, Tokyo, Japan).

Kurosaka Y., Shiroya Y., Yamauchi H., Kaneko T., Okubo Y., Shibuya K, & Minato K. (2015). Effects of habitual exercise and dietary restriction on intrahepatic and periepididymal fat accumulation in Zucker fatty rats. BMC Research Notes, 8, 121.

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Quantification of Serum Free Fatty Acids

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Serum levels of FFAs were measured using commercially available kits according to the manufacturer’s instructions (NEFA E-test, Wako Pure Chemical Industries Ltd., Osaka, Japan).

Kurosaka Y., Machida S., Shiroya Y., Yamauchi H, & Minato K. (2021). Protective Effects of Voluntary Exercise on Hepatic Fat Accumulation Induced by Dietary Restriction in Zucker Fatty Rats. International Journal of Molecular Sciences, 22(4), 2014.

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Hepatic Biomarker Determination Protocol

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Serum ALT and aspartate aminotransferase (AST) levels were determined using Transaminase CII-B-test Wako and ALP levels were Alkali-phospha B-test Wako commercial kits (Wako Pure Chemicals, Osaka, Japan). The hepatic samples were prepared as previously described [29 (link)]. Hepatic TG, FFA, and TC were determined using the Triglyceride E-test Wako, NEFA E-test Wako, and Cholesterol E-test Wako kits (Wako Pure Chemicals), respectively.

Miyata M., Matsushita K., Shindo R., Shimokawa Y., Sugiura Y, & Yamashita M. (2020). Selenoneine Ameliorates Hepatocellular Injury and Hepatic Steatosis in a Mouse Model of NAFLD. Nutrients, 12(6), 1898.

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