The lncRNA and mRNA microarray expression profiling was performed in articular cartilage samples from OA patients of Han nationality and Tibetans. Briefly, total RNA (100 ng) was labeled with the mRNA Complete Labeling and Hyb Kit (Agilent Technologies) and hybridized on the SurePrint G3 Human Gene Expression 8x60K v2 Microarray (Agilent Technologies). The microarray contains 50,599 probes for 32,776 human mRNA and 17,438 human lncRNAs, which are derived from authoritative databases, including RefSeq, Ensemble, GenBank, and the Broad Institute. After hybridization and washing, processed slides were scanned with the Agilent G2505C microarray scanner (Agilent Technologies). Raw data were extracted using Feature Extraction (version10.7.1.1; Agilent Technologies). Next, quantile normalization and subsequent data processing were done through the Genespring software (version 12.0; Agilent Technologies). The microarray profiling was conducted in Beijing Capitalbio Technology company.
Mrna complete labeling and hyb kit
Manufactured by Agilent Technologies
The mRNA Complete Labeling and Hyb Kit is a laboratory product designed for the labeling and hybridization of mRNA samples. It provides the necessary reagents and materials to facilitate the preparation of labeled mRNA for use in various molecular biology applications.
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Lab products found in correlation
2 protocols using mrna complete labeling and hyb kit
1
Profiling lncRNA and mRNA in Osteoarthritis
2
Microarray Analysis of Liver Hydatid Disease
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Microarray expression profiling was conducted in liver tissue from healthy
controls (n = 6) and in patients with liver hydatid disease (n = 6). Briefly,
total RNA (100 ng) was labeled with the mRNA Complete Labeling and Hyb Kit
(Agilent Technologies) and hybridized on the SurePrint G3 Human Gene Expression
8x60K v2 microarray (Agilent Technologies). The microarray contains 50,599
probes for 32,776 human mRNAs originating from authoritative databases,
including RefSeq, Ensemble, GenBank, and the Broad Institute. After
hybridization and washing, processed slides were scanned with the Agilent G2505C
microarray scanner (Agilent Technologies).
controls (n = 6) and in patients with liver hydatid disease (n = 6). Briefly,
total RNA (100 ng) was labeled with the mRNA Complete Labeling and Hyb Kit
(Agilent Technologies) and hybridized on the SurePrint G3 Human Gene Expression
8x60K v2 microarray (Agilent Technologies). The microarray contains 50,599
probes for 32,776 human mRNAs originating from authoritative databases,
including RefSeq, Ensemble, GenBank, and the Broad Institute. After
hybridization and washing, processed slides were scanned with the Agilent G2505C
microarray scanner (Agilent Technologies).
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