Originpro 8 sr4

Statistical analyses were performed using OriginPro 8 SR4 (v8.0951, Northampton, MA, USA). To compare various rates between groups (oviposition, hatching, molting, etc.), Fisher’s exact test or the chi-square test was used. For other data, the significance of differences between groups was estimated according to the Mann–Whitney U test (for 2 groups) and the Kruskal–Wallis H test (for 3 or more groups). For multiple comparisons, Bonferroni correction was applied.

Pooled prevalence was calculated using a maximum likelihood estimator with the assumption of 100% test sensitivity and specificity (Williams and Moffitt, 2001 (link)). The calculations were performed on the EPITOOLS web platform (Sergeant, 2018 ). In cases, when all the analyzed pools were positive, then the LaPlace adjustment method was used (confidence interval calculator for a completion rate). Statistical analyses were performed using OriginPro 8 SR4 (v8.0951, Northampton, MA, USA). To compare TBEV prevalence between groups, Fisher’s exact test or chi-square test were used. For other data, the significance of differences between groups was estimated according to the Mann–Whitney U-test (for two groups) and the Kruskal–Wallis H-test (for three groups). For multiple comparisons correction, Bonferroni correction was applied.

Quantitative data are expressed as the mean ± standard deviation of at least three independent experiments. For statistical analysis, one-way analysis of variance with Bonferroni correction was performed by using OriginPro 8 SR4 software (version 8.0951; OriginLab Corporation, Northampton, MA, USA). Data normality was tested by Shapiro-Wilk test. A P value of less than 0.05 was considered statistically significant.

Quantitative data were expressed as the means ± SD of at least three independent experiments. For statistical analysis, one-way ANOVA with Bonferroni correction was performed using OriginPro 8 SR4 software (version 8.0951; OriginLab Corporation, Northampton, MA, USA) if there were more than three groups. p < 0.05 was considered statistically significant.
Additional methods are available in Additional file 1.

Quantitative data were expressed as the mean ± S.E.M (standard error of measurement) of at least 3 independent experiments. For statistical analysis, one-way ANOVA with Bonferroni correction was performed using the OriginPro 8 SR4 software (ver. 8.0951, OriginLab Corporation, Northampton, MA, USA). A p value of less than 0.05 was considered to be statistically significant.

All of the luminescence measurements were carried out at room temperature. We used a Fluorolog FL3-22 spectrofluorimeter (Horiba Jobin Yvon, Longjumeau, France) with double-grating excitation and emission monochromators for luminescence measurements over 400 to 700 nm wavelength range with a 0.1 nm step. PL spectra deconvolutions were carried out with OriginPro 8 SR4 (OriginLab Corp., Northampton, MA, USA) software using the Fit Multiple Peak procedure. The luminescence decay kinetics were studied by the excitation of a pulsed diode laser (λ = 377 nm, Δτ = 1.5 ns) and a Xenon 450W Ushio UXL-450S/O lamp (355 nm). Processing of the luminescence decay curves was carried out using the Fit Exponential procedure of an OriginPro 8 SR4 software. All of the decay curves were described by two exponentials (criterion Adj. R-Square > 0.998). The final data were averaged over 5 measurements.