Ispinesib
Manufactured by MedChemExpress
Sourced in United States
Ispinesib is a small molecule inhibitor that targets the mitotic kinesin Eg5. It functions by inhibiting the ATPase activity of Eg5, which is essential for the formation and maintenance of the mitotic spindle during cell division.
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Lab products found in correlation
Epothilone b, by MedChemExpress (2 mentions)
Filanesib, by MedChemExpress (1 mentions)
Paclitaxel, by MedChemExpress (1 mentions)
Cabazitaxel, by MedChemExpress (1 mentions)
Cytarabine, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Carl Roth (1 mentions)
Gilteritinib, by MedChemExpress (1 mentions)
Quizartinib, by Cayman Chemical (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Ponatinib, by Cayman Chemical (1 mentions)
Cabozantinib, by Selleck Chemicals (1 mentions)
Venetoclax, by MedChemExpress (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Bortezomib, by Selleck Chemicals (1 mentions)
4 protocols using ispinesib
1
Evaluating Small Molecule Inhibitors
2
Cell Viability Assay with Cytotoxic Agents
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Cell viability was determined by CCK8 cytotoxicity assay. Briefly, cells were seeded in 96-well plates at 5000 cells/well and treated with at 37°C with Ispinesib (0, 30, 60, 120 or 240 nM, MedChem Express, Monmouth Junction, NJ, USA, Cat No.HY-50759), Paclitaxel (0, 10, 20, 40 or 80 nM, MedChem Express, Monmouth Junction, NJ, USA, Cat No. HY-B0015) and Epothilone-b (0, 40, 80, 160 or 320 nM, MedChem Express, Monmouth Junction, NJ, USA, Cat No. HY-17029) for 24 h, respectively. Cells were incubated with CCK8 (10 μl/well) for 2 h at 37°C and then spectrophotometrically quantified at 450 nm.
3
Evaluating Cell Viability and Sensitivity
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We performed a CCK-8 assay (Cellcook, Guangzhou, China, Cat No. CT01A) using the manufacturer’s guidelines to determine the viability of cells transfected with DDIT4-siRNA and siRNA-NC. We seeded these cells in the logarithmic growth phase into 96-well plates. In order to evaluate the effect on cell proliferation capacity, 10 μl CCK-8 reagent was added in all wells and incubated for 2 hours at 37°C at 0, 24, 48, 72 h after culturing. For the sensitivity of cells to the drug, cells were treated at 37˚C with Ispinesib (0, 20, 40, 80 or 100 nM, MedChem Express, Monmouth Junction, NJ, USA, Cat No.HY-50759), Cabazitaxel (0, 10, 20, 40 or 80 nM, MedChem Express, Monmouth Junction, NJ, USA, Cat No. HY-15459) and Epothilone-b (0, 40, 80, 160 or 320 nM, MedChem Express, Monmouth Junction, NJ, USA, Cat No. HY-17029) for 24 h, respectively. 10 μl CCK-8 reagent was added in all wells and incubated for 2 hours at 37°C. Finally, we measured the absorbance of each well at 450 nm using a microplate reader.
4
Cell Culture and Drug Preparation
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All cell lines were purchased from DSMZ (Braunschweig, Germany) or ATCC (Manassas, VA, USA). The cell lines were regularly tested to exclude mycoplasma contamination and authenticated. All cell lines were grown at 37 °C and 5% CO2. A549 cells were cultured in DMEM medium (Gibco™, Thermo Fisher Scientific, Waltham, MA, USA). All other cell lines were cultured in RPMI 1640 medium (Gibco™, Thermo Fisher Scientific). Both media were supplemented with 10% fetal bovine serum (FBS), 10 U/mL penicillin, 10 µg/mL streptomycin and 2 mM L-glutamine (all Gibco™, Thermo Fisher Scientific). Bortezomib (S1013; Selleck Chemicals, Houston, TX, USA), cabozantinib (S1119; Selleck Chemicals), cytarabine (PHR1787; Millipore Sigma, Burlington, MA, USA), gilteritinib (HY-12432; MedChemExpress, Monmouth Junction, NJ, USA), ispinesib (HY-50759; MedChemExpress), midostaurin (M1323; Millipore Sigma), ponatinib (11494; Cayman Chemical, Ann Arbor, MI, USA), quizartinib (17986; Cayman Chemical), venetoclax (HY-15531; MedChemExpress) and WS6 (S7442; Selleck Chemicals) were dissolved in dimethyl sulfoxide (DMSO; Carl Roth, Karlsruhe, Germany) and diluted further in culture medium immediately before use.
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