Anti-LGR5 (ab75850) and anti-phospho-Smad2 (Ser465/467) (ab3849) antibodies were purchased from Abcam, Cambridge, MA, USA. Anti-cleaved PARP (5625), anti-cleaved caspase 9 (9502), anti-Smad2/3 (8685), anti-Smad4 (9515), anti-TGFβ RII (11888) and anti-actin (4970) antibodies were obtained from Cell Signaling Technology, Danvers, MA, USA. Anti-TGFβ RI (sc-398) was from Santa Cruz, Dallas, Texas, USA. Anti-Flag (F1804) and anti-HA (MMS-101P) antibodies were purchased from Sigma and Biolegend respectively. Recombinant human TGFβ1 and RSPO1 were purchased from R&D Systems, Minneapolis, MN, USA and Origene, Rockville, MD, USA. The TGFβ RI kinase inhibitor (SB525334) and the human RSPO1 neutralizing antibody (AF4645), which is antigen affinity-purified and displays less than 1% cross-reactivity with other human RSPOs, were purchased from Sigma and R&D Systems respectively.
Anti ha mms 101p
Manufactured by Merck Group
The Anti-HA (MMS-101P) is a laboratory reagent used for the detection and purification of proteins tagged with the hemagglutinin (HA) epitope. It is a monoclonal antibody that specifically binds to the HA tag, allowing for the identification and isolation of HA-tagged proteins from complex samples.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (2 mentions)
Sb 525334, by Merck Group (1 mentions)
Anti actin 4970, by Cell Signaling Technology (1 mentions)
Anti flag f1804, by Merck Group (1 mentions)
Anti gst, by Proteintech (1 mentions)
Ecl chemiluminescence reagent, by Thermo Fisher Scientific (1 mentions)
Biotin 16 utp, by Roche (1 mentions)
Pierce rna 3 end biotinylation kit, by Thermo Fisher Scientific (1 mentions)
Anti dgcr8, by Proteintech (1 mentions)
Scriptmax thermo t7 transcription kit, by Toyobo (1 mentions)
Normal mouse igg, by Santa Cruz Biotechnology (1 mentions)
Chemiluminescent nucleic acid detection module, by Thermo Fisher Scientific (1 mentions)
Dynabeads myone streptavidin c1, by Thermo Fisher Scientific (1 mentions)
Glutathione sepharose 4b, by GE Healthcare (1 mentions)
Flag peptide, by Merck Group (1 mentions)
Polybrene, by Merck Group (1 mentions)
Puromycin, by Yeasen (1 mentions)
2 protocols using anti ha mms 101p
1
TGFβ/RSPO1 Signaling Pathway Inhibition
2
Antibody-based Identification of RNA Modifications
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibody against anti-ac4C (#ab252215) was from Abcam. Antibodies against anti-NAT10 (#sc-271770) and normal mouse IgG (#sc-2025) were from Santa Cruz Biotechnology. Antibodies against anti-Flag M2 (#F1804) and anti-HA (#MMS-101 P) were from Sigma-Aldrich. Antibodies against anti-GAPDH (60004-1-Ig), anti-Tubulin (#66031-1-Ig), anti-DGCR8 (#60084-1-Ig), anti-DROSHA (27958-1-AP), normal rabbit IgG (#30000-0-AP) and anti-GST (#66001-2-Ig) were from ProteinTech Group. Puromycin (#58-58-2) was from YEASEN. 3 × Flag peptide (#F4799) and Polybrene (#H9268) were from Sigma-Aldrich. ECL chemiluminescence reagent (#32106), Lipofectamine 2000 Transfection Reagent (#11668019), Chemiluminescent Nucleic Acid Detection Module (#89880), Pierce™ RNA 3' End Biotinylation Kit (#20160) and were from ThermoFisher Scientific. Protein G Plus/Protein A agarose suspension (#IP05) was from Calbiochem. Glutathione Sepharose 4B (#17-0756-01) was from GE Healthcare Life Sciences. Dynabeads MyOne Streptavidin C1 (#65001) was from Invitrogen. N4-acetylcytidine triphosphate (#HY-111815) were from MedChemExpress. ScriptMAX™ Thermo T7 Transcription Kit (#TSK-101) was from TOYOBO. Biotin-16-UTP (#11388908910) was from Roche. Primers and probes tagged with biotin were from Sangon Biotech and listed in Supplementary Table S1 .
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