Super q water system

Manufactured by Merck Group

Sourced in Morocco

The Super-Q water system is a laboratory equipment designed to produce high-purity deionized water. It utilizes a multi-stage filtration process to remove contaminants, resulting in water that meets the stringent purity requirements for various laboratory applications.

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Lab products found in correlation

Chloroform, by Avantor (4 mentions) Acetonitrile, by Avantor (4 mentions) Methanol, by Avantor (4 mentions) Anhydrous 1 4 dioxane, by Avantor (3 mentions) Uric acid, by Merck Group (2 mentions) Chitosan, by Merck Group (2 mentions) Diethyl ether, by Avantor (2 mentions) Nipaam, by Merck Group (2 mentions) Dimethyl γ butyrolactone acrylate dba glycidyl methacrylate gma acrylic acid 2 2 azobis 2 methylpropionitrile azobisisobutyronitrile aibn n n methylenebisacrylamide mba piperazine pip glycine, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Cambrex (2 mentions) Live dead viability cytotoxicity kit, by Thermo Fisher Scientific (2 mentions) Glutaraldehyde, by Merck Group (2 mentions) Low glucose dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (2 mentions) Antibiotic antimycotic, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Gemini Bio (2 mentions) K2ircl6 4, by Merck Group (2 mentions) Ascorbic acid, by Merck Group (2 mentions) Clozapine, by Merck Group (1 mentions) Ferrocyanide, by Merck Group (1 mentions) Ferricyanide, by Merck Group (1 mentions)

14 protocols using super q water system

Chitosan-based Bioconjugate Preparation

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Chitosan, clozapine, norclozapine, and uric acid were purchased from Sigma-Aldrich. Graphene (N002-PDR) was purchased from Angstron Materials. Deionized water (>18 MΩ) was obtained from Super-Q water system (Millipore). Chitosan solution (1.1%; pH 5.3) was prepared by dissolving Chitosan flakes in deionized water and the pH was adjusted using an HCl solution. A stock solution of clozapine (5 mM) was prepared in methanol and stored at −80 °C. Standard clozapine solutions were prepared by diluting this stock solution with serum of healthy volunteers.

Kang M., Kim E., Winkler T.E., Banis G., Liu Y., Kitchen C.A., Kelly D.L., Ghodssi R, & Payne G.F. (2017). Reliable Clinical Serum Analysis with Reusable Electrochemical Sensor: Toward Point-of-Care Measurement of the Antipsychotic Medication Clozapine. Biosensors & bioelectronics, 95, 55-59.

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Osteogenic Hydrogel Scaffold Synthesis

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NiPAAm, dimethyl-γ-butyrolactone acrylate (DBA), glycidyl methacrylate (GMA), acrylic acid (AA), 2,2’-azobis(2-methylpropionitrile) (azobisisobutyronitrile, AIBN), N,N’-methylenebisacrylamide (MBA), piperazine (PiP), glycine, and glutaraldehyde were purchased from Sigma Aldrich (Sigma, St. Louis, MO) and used as received. Anhydrous 1,4-dioxane, diethyl ether, and acetone in analytical grade; and water, acetonitrile, chloroform, and methanol in HPLC-grade were purchased from VWR (Radnor, PA) and used as received. PBS (powder, pH 7.4) was obtained from Gibco Life, Grand Island, NY. Ultrapure water was obtained from a Millipore Super-Q water system (Millipore, Billerica, MA). Acidic gelatin (IEP=5.0) was obtained from Nitta Gelatin (Osaka, Japan). Complete osteogenic medium (COM) was made from minimal essential medium α modification (αMEM) (Gibco Life, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS) (Cambrex BioScience, Walkersville, MD), 10⁻⁸ M dexamethasone, 10 mM β-glycerol 2-phosphate, 50 mg/L ascorbic acid, and 10 mL/L antibiotic-antimycotic solution (Gibco, Life, Grand Island, NY). Live/Dead viability/cytotoxicity kit was purchased from Molecular Probes (Eugene, OR). The calcium assay was purchased from Genzyme Diagnostics (Cambridge, MA).

Vo T.N., Shah S.R., Lu S., Tatara A.M., Lee E.J., Roh T.T., Tabata Y, & Mikos A.G. (2015). Injectable Dual-Gelling Cell-Laden Composite Hydrogels for Bone Tissue Engineering. Biomaterials, 83, 1-11.

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Electrochemical Characterization of Microelectrodes

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The following materials have been used without further purification: OLZ (catalog number: 1478301, Merck), Dihydrogen hexachloroplatinate (IV) hexahydrate (chloroplatinic acid; catalog number: 011051, Alfa aesar), 99% Lead(II) acetate trihydrate (lead acetate; catalog number; A11746, Alfa Aesar), di-sodium hydrogen phosphate dihydrate (catalog number: 1.06580.1000, Merck), sodium dihydrogen phosphate dihydrate (catalog number: 1.06342.0250, Merck), sodium chloride (catalog number: 1.06404.1000, Merck), potassium hexacyanoferrate(II) trihydrate (‘Ferrocyanide’, catalog number: 1.04984.0100, Merck), potassium hexacyanoferrate(III) (‘Ferricyanide’, catalog number: 1.04973.0100, Merck), acetone Find Bio-Lab, Ltd.), 2-propanol (catalog number: 001626052100, Bio-Lab, Ltd.) and hydrochloric acid 32% (catalog number: 000846050100, Bio-Lab, Ltd.). Ultra-pure water (> 18 MΩ) was obtained from a Super Q water system (Millipore). All prepared solutions were diluted in PBS (10 mM, pH 7.4) solution. For all the electrochemical tests, a three-electrode electrochemical cell, consisting of an Ag/AgCl reference electrode (a metal wire coated with Ag/AgCl ink; catalogue number: 011464, BAS, Inc.), a ring platinum counter electrode (catalogue number: 012961, ALS Co., Ltd.), and microfabricated microelectrodes as working electrodes were used.

Shukla R.P., Belmaker R.H., Bersudsky Y, & Ben-Yoav H. (2020). A platinum black-modified microelectrode for in situ olanzapine detection in microliter volumes of undiluted serum. Journal of Neural Transmission, 127(2), 291-299.

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Rabbit Tissue Preparation Protocol

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Fetal bovine serum (FBS) was purchased from Gemini Bio-Products (Sacramento, CA). Low glucose Dulbecco’s modified eagle medium (DMEM) and antibiotic-antimycotic were purchased from ThermoFisher Scientific (Waltham, MA). Phosphate buffered saline (PBS) and 10% neutral buffered formalin were purchased from MilliporeSigma (St. Louis, MO). New Zealand White (NZW) rabbits were purchased from Charles River Laboratories (Wilmington, MA). Lactated Ringer’s Solution (LRS), ethylenediaminetetraacetic acid (EDTA) solution, and all other described surgical supplies and substances were purchased from Patterson Veterinary Supply (Devens, MA). Ultrapure water was obtained from a Millipore Super-Q water system (Billerica, MA).

Guo J.L., Kim Y.S., Koons G.L., Lam J., Navara A.M., Barrios S., Xie V.Y., Watson E., Smith B.T., Pearce H.A., Orchard E.A., van den Beucken J.J., Jansen J.A., Wong M.E, & Mikos A.G. (2021). Bilayered, Peptide-Biofunctionalized Hydrogels for In Vivo Osteochondral Tissue Repair. Acta biomaterialia, 128, 120-129.

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Synthesis of Stimuli-Responsive Hydrogels

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N-Isopropylacrylamide (NiPAAm), glycidyl methacrylate (GMA), 2,2′-azobis(2-methylpropionitrile) (azobisisobutyronitrile, AIBN), N,N′-methylenebisacrylamide (MBA), and piperazine (PiP) were purchased from Sigma-Aldrich (Sigma, St. Louis, MO) and used as received. The solvents−tetrahydrofuran (THF), dimethylformamide (DMF), diethyl ether, and acetone in analytical grade and water, acetonitrile, chloroform, and methanol in HPLC grade–were obtained from VWR (Radnor, PA) and used as received. Phosphate buffered saline (PBS) solution was mixed from powder (pH 7.4, Gibco Life, Grand Island, NY), and ultrapure water was obtained from a Millipore Super-Q water system (Millipore, Billerica, MA).

Tzouanas S.N., Ekenseair A.K., Kasper F.K, & Mikos A.G. (2014). Mesenchymal Stem Cell and Gelatin Microparticle Encapsulation in Thermally and Chemically Gelling Injectable Hydrogels for Tissue Engineering. Journal of biomedical materials research. Part A, 102(5), 1222-1230.

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Thermo-Responsive Copolymer Synthesis and Characterization

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N-isopropylacrylamide (NiPAAm), dimethyl-γ-butyrolactone acrylate (DBA), glycidyl methacrylate (GMA), acrylic acid (AA), 2,2′-azobis(2-methylpropionitrile) (azobisisobutyronitrile, AIBN), N,N’-methylenebisacrylamide (MBA), and piperazine (PiP) were purchased from Sigma Aldrich (Sigma, St. Louis, MO) and used as received. Anhydrous 1,4-dioxane, dimethylformamide, diethyl ether, and acetone in analytical grade; water, acetonitrile, chloroform, and methanol in HPLC-grade; and 1 N sodium hydroxide were purchased from VWR (Radnor, PA) and used as received. Phosphate buffered saline (PBS) solution was obtained from Gibco Life, Grand Island, NY (powder, pH 7.4). Ultrapure water was obtained from a Millipore Super-Q water system (Millipore, Billerica, MA).

Vo T.N., Ekenseair A.K., Spicer P.P., Watson B.M., Tzouanas S.N., Roh T.T, & Mikos A.G. (2014). In Vitro and In Vivo Evaluation of Self-Mineralization and Biocompatibility of Injectable, Dual-Gelling Hydrogels for Bone Tissue Engineering. Journal of controlled release : official journal of the Controlled Release Society, 205, 25-34.

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Hemostatic Alginate-Chitosan Microparticles

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Sodium alginate, zeolite (particle size < 45 μm), calcium chloride, acetic acid, and phosphate buffered saline (PBS, 1×) were purchased from Sigma-Aldrich (St. Louis, MO). Chitosan (MW 100–300 kDa) was purchased from Acros Organics (Fair Lawn, NJ). Ultra-pure deionized (DI) water was obtained from a Millipore Super-Q water system (Billerica, MA). Citrated sheep blood was purchased from Hemostat Laboratories (Dixon, CA) and was received and stored away from direct light at 3–88C following manufacturer’s instructions. Blood was used within one week after arrival. Plastic-capped glass vials (5 mL) used in the study were purchased from VWR Scientific (West Chester, PA). Whole human blood for scanning electron microscopy (SEM) imaging was collected in several 2.7 mL citrate tubes at the University of Maryland Health Center in concordance with the University of Maryland Institutional Review Board [400316–3] titled “zeolite Loaded Chitosan and Alginate Microparticles Designed for Hemostatic Use.” MTS CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, Madison, WI) and Live/Dead Cell Imaging Kit (Life Technologies, Carlsbad, CA), were used in cell viability testing. All materials were used as they were received unless otherwise described.

Fathi P., Sikorski M., Christodoulides K., Langan K., Choi Y.S., Titcomb M., Ghodasara A., Wonodi O., Thaker H., Vural M., Behrens A, & Kofinas P. (2017). Zeolite-loaded alginate-chitosan hydrogel beads as a topical hemostat. Journal of biomedical materials research. Part B, Applied biomaterials, 106(5), 1662-1671.

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Biopolymer-Based Microparticle Synthesis

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The following materials were purchased from
Sigma-Aldrich; alginic acid sodium salt from brown algae (medium viscosity),
gelatin from porcine skin (type A and type B), S-adenosylhomocysteine
(SAH), phosphate buffered saline (PBS) tablets, and calcium chloride
anhydrous beads. Microbial transglutaminase (mTG; Activa TI; 100 U/g
as reported by the manufacturer) was obtained from Ajinomoto (Japan).
Fluorescent FITC labeled polystyrene microparticles (0.84 μm,
0.1% w/v) were purchased from Spherotech Inc. (Lake Forest, IL). Water
was deionized (DI) with Millipore SUPER-Q water system until final
resistivity >18 MΩ·cm was reached. Buffer and calcium
chloride
solutions were filtered with a Fisherbrand sterilized syringe filter
(0.22 μm) before use.

Liu Y., Wu H.C., Chhuan M., Terrell J.L., Tsao C.Y., Bentley W.E, & Payne G.F. (2015). Functionalizing Soft Matter for Molecular Communication. ACS Biomaterials Science & Engineering, 1(5), 320-328.

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Preparation of Iridium Oxide Stock Solution

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The following were purchased from Sigma-Aldrich: K₂IrCl₆ (IV), and phosphate buffered saline (PBS, pH 7.4). The water (>18 MΩ) used in this study was obtained from a Super Q water system (Millipore). A stock solution of 10 mM K₂IrCl₆ (Ir^OX) was prepared in PBS (pH 7.4) and its aliquot was stored in −80 °C freezer.

Kim E., Zhao Z., Rzasa J.R., Glassman M., Bentley W.E., Chen S., Kelly D.L, & Payne G.F. (2021). Association of acute psychosocial stress with oxidative stress: Evidence from serum analysis. Redox Biology, 47, 102138.

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Synthesis and Characterization of Melanin

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The following were purchased from Sigma-Aldrich: chitosan, 1,1′-ferrocenedimethanol (Fc), Ru(NH₃)₆Cl₃ (Ru³⁺), and K₃IrCl₆ (Ir³⁺). The water (>18 MΩ) used in this study was obtained from a Super Q water system (Millipore). chitosan solutions (1%, pH 5.5) were prepared by dissolving chitosan flakes in HCl to achieve a final pH of 5–6. The solutions of mediator were prepared in phosphate buffer (0.1 M; pH 7.0). Previously described methods were used to prepare the synthetic eumelanin62 (link) and pheomelanin35 (link). The natural melanin samples were purified from human hairs as previously reported9 (link).

Kim E., Panzella L., Micillo R., Bentley W.E., Napolitano A, & Payne G.F. (2015). Reverse Engineering Applied to Red Human Hair Pheomelanin Reveals Redox-Buffering as a Pro-Oxidant Mechanism. Scientific Reports, 5, 18447.

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