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Acetyl foxo1

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Acetyl-FoxO1 is a laboratory reagent produced by Santa Cruz Biotechnology. It is a post-translational modification of the FoxO1 protein, specifically the acetylation of FoxO1. FoxO1 is a transcription factor involved in various cellular processes. The Acetyl-FoxO1 product provides a modified version of the FoxO1 protein for use in research applications.

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Lab products found in correlation

Foxo1, by Cell Signaling Technology (3 mentions) Acetyl p53, by Cell Signaling Technology (2 mentions) Sirt1, by Cell Signaling Technology (2 mentions) Sirt6, by Cell Signaling Technology (2 mentions) Sirt1, by Merck Group (1 mentions) Gapdh, by Merck Group (1 mentions) Enhanced chemiluminescence kit, by Merck Group (1 mentions) Hydroxylated hif 1α, by Cell Signaling Technology (1 mentions) Quantione imaging software, by Bio-Rad (1 mentions) β actin, by Abcam (1 mentions) Endothelial cell growth supplement (ecgs), by Merck Group (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Sapk jnk, by Cell Signaling Technology (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions) Sirt3, by Cell Signaling Technology (1 mentions) 2 7 dichlorofluorescein diacetate dcfh2 da, by Merck Group (1 mentions) P16ink4a, by Cell Signaling Technology (1 mentions) P21cip1, by Cell Signaling Technology (1 mentions) Smp30, by Santa Cruz Biotechnology (1 mentions)

4 protocols using acetyl foxo1

1

Western Blot Analysis of Acetylated Proteins

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Cell extracts were prepared using RIPA buffer, resolved on 7.5 or 12.5% polyacrylamide gels, transferred to nitrocellulose, and hybridized using antibodies against acetyl‐FOXO1 (sc‐49437, 1:200; Santa Cruz Biotechnology, Santa Cruz, CA, USA), acetyl‐p53 (#2570, 1:1000; Cell Signaling Technology, Danvers, MA, USA), actin (sc‐1616, 1:3000; Santa Cruz Biotechnology), FOXO1 (#9454, 1:1000; Cell Signaling Technology), Gapdh (G9545, 1:10000; Sigma‐Aldrich), p16 (sc‐1207, 1:200; Santa Cruz Biotechnology), p21 (sc‐6246, 1:50; Santa Cruz Biotechnology), p53 (#2524, 1:200; Cell Signaling Technology), SIRT1 (07–131, 1:200; Millipore Corporation, Billerica, MA, USA), and TERT (ab104588, 1:200; Abcam, Cambridge, UK).
Kondo H., Kim H.W., Wang L., Okada M., Paul C., Millard R.W, & Wang Y. (2015). Blockade of senescence‐associated microRNA‐195 in aged skeletal muscle cells facilitates reprogramming to produce induced pluripotent stem cells.. Aging Cell, 15(1), 56-66.
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2

Protein Expression Analysis in Gastrocnemius and HUVECs

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Gastrocnemius tissue and HUVECs were lysed to extract proteins. Protein lysates (50 μg) were loaded and separated on the SDS‐PAGE gel. Then, proteins were transferred onto nitrocellulose membranes. Membranes were incubated with the corresponding primary antibodies against cleaved caspase‐3 (Abcam, ab49822), caspase‐3 (Abcam, ab13847), Bcl‐2 (Abcam, ab182858), BAX (Abcam, ab32503), LXRα (Abcam, ab176323), LXRβ (Abcam, ab28479), NOX4 (Abcam, ab109225), 3‐Nitrotyrosine (Abcam, ab52309), SIRT1 (Cell Signaling, #9475), hydroxylated HIF‐1α (Cell Signaling, #3434), FoxO1 (Cell Signaling, #2880), acetyl‐FoxO1 (Santa Cruz, sc‐49437), p53 (Cell Signaling, #2524), acetyl‐p53 (Cell Signaling, #2570) and β‐actin (Abcam, ab8227). Membranes were treated with an enhanced chemiluminescence kit (Millipore) and were imaged with UVP Bio‐Imaging Systems. QuantiOne imaging software (Bio‐Rad) was used for quantitative analysis by evaluating the integrated optical density.
Fan W., Zhang R., Han D., Jiang Z., Li S., Zhang J., Li Y., Wang Y, & Cao F. (2020). Reduced Sirtuin1 signalling exacerbates diabetic mice hindlimb ischaemia injury and inhibits the protective effect of a liver X receptor agonist. Journal of Cellular and Molecular Medicine, 24(10), 5476-5490.
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3

Histological Analysis of Murine Paw Inflammation

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Paraffin sections of murine paws were stained with H&E, Safranin-O, and tartrate-resistant acid phosphatase (TRAP) for evaluation of inflammation and joint destruction. After deparaffinization, tissue sections were immunostained with antibodies against CD68 (Santa Cruz Biotechnology, Dallas, TX, USA), Ly6G (Abcam, Cambridge, UK), Sirt6 (Cell Signaling Technology, Beverly, MA, USA), FoxO1 (Cell Signaling Technology) or acetyl-FoxO1 (Santa Cruz Biotechnology).
Woo S.J., Noh H.S., Lee N.Y., Cheon Y.H., Yi S.M., Jeon H.M., Bae E.J., Lee S.I, & Park B.H. (2018). Myeloid sirtuin 6 deficiency accelerates experimental rheumatoid arthritis by enhancing macrophage activation and infiltration into synovium. EBioMedicine, 38, 228-237.
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4

Antcins Isolation and Characterization

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Antcin A, antcin B, antcin C, antcin H, antcin K and antcin M were isolated from the fruiting bodies of A. cinnamomea and A. salmonea as described previously [17 (link)]. The purity of the antcins was above 99% as confirmed by HPLC and FT-NMR analysis. Minimum essential medium (MEM), Medium 199 (M-199), fetal bovine serum (FBS), sodium pyruvate, penicillin and streptomycin were obtained from Invitrogen (Carlsbad, CA). Heparin sodium salt, endothelial cell growth supplement (ECGS), N-acetylcysteine, 2′, 7′-dichlorofluorescein diacetate (DCFH2-DA), 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and D-Glucose were purchased from Sigma-Aldrich (St Louis, CA). Antibodies against cyclin D1, cyclin B1, cyclin E, CDK2, CDK4, CDK6, Cdc2, phos-pRb, p16INK4A, p21CIP1, acety-p52, phos-p53, phos-FoxO1, FoxO1, phos-JNK/SAPK, JNK/SAPK, phos-p38 MAPK, p38 MAPK, phos-ERK1/2, ERK1/2, Phos-AKT, AKT, histone H3, phos-SIRT-1, SIRT-1, SIRT-3, SIRT-6 and Keap-1 were obtained from Cell Signaling Technology, Danvers, MA. Antibodies against p53, SMP30 and acetyl-FoxO1 were purchased from Santa Cruz Biotechnology, Dallas, TX. Antibodies against HO-1 and NQO-1 were obtained from Abcam, Cambridge, UK. All other chemicals were reagent grade or HPLC grade and supplied by either Merck (Darmstadt, Germany) or Sigma-Aldrich.
Senthil K.K., Gokila V.M., Mau J.L., Lin C.C., Chu F.H., Wei C.C., Liao V.H, & Wang S.Y. (2016). A steroid like phytochemical Antcin M is an anti-aging reagent that eliminates hyperglycemia-accelerated premature senescence in dermal fibroblasts by direct activation of Nrf2 and SIRT-1. Oncotarget, 7(39), 62836-62861.
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