Ten milliliters of fasting blood were drawn from all subjects, and serum was separated by centrifugation at 3,000 rpm for 15 minutes and stored in a freezer at -70°C until analysis. The serum samples were analyzed for TALP (Autoanalyzer, Toshiba, Japan), OC (Osteo-RIACT, CIS bio international, France), and 1CTP (UniQ ICTP RIA, Orion Diagnostica, Finland).
Autoanalyzer
Manufactured by Toshiba
Sourced in Japan
The Autoanalyzer is an automated analytical instrument used for the quantitative determination of various analytes in fluid samples, such as blood, urine, or other biological fluids. The core function of the Autoanalyzer is to perform automated chemical analyses, providing accurate and consistent results.
Automatically generated - may contain errors
8 protocols using autoanalyzer
1
Fasting Serum Biomarker Analysis
2
Serum Enzyme Analysis Workflow
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Collected blood was centrifuged at 1,000 × g for 15 min at 4°C to obtain serum that was analyzed for quantification of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) using commercial kits (cat. no. ALT000A, AST000S and LDH000S for ALT, AST and LDH, respectively; from Beijing Autobio Co. Ltd.) following the manufacturer's protocol on Auto-Analyzer (TBA-120FR; TOSHIBA).
3
Cardiometabolic Biomarker Measurements
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Systolic and diastolic blood pressures were measured on the left upper arm after 5 minutes of rest in a sitting position using an automatic blood pressure monitor (HEM-907-E; OMROM, Tokyo, Japan). Abdominal circumference was measured in a standing position at the midway between the lower costal margin and the iliac crest. After fasting for 8 hours or more, blood was drawn from the antecubital vein of each participant. The samples were properly processed, refrigerated at 2℃ to 8℃, and analyzed within 24 hours of transportation. Fasting glucose was measured using a Hitachi Automatic Analyzer 7600 (Hitachi, Tokyo, Japan). High-performance liquid chromatography-723G7 (Tosoh, Tokyo, Japan) was used to check HbA1c. Insulin concentrations were estimated using an electrochemiluminescence method (COBAS E 411; Roche Diagnostics, Mannheim, Germany). The intra-assay and interassay coefficients of variation for the biochemical assays ranged between 3.1% and 7.6%. The blood levels of total cholesterol, triglycerides, high density lipoprotein, and low density lipoprotein cholesterol levels were measured using a Toshiba 200FR Autoanalyser (Toshiba Medical Systems Co. Ltd., Tokyo, Japan). High-sensitivity C-reactive protein was measured with an immunoturbidimetric method (CRP II Latex X2; Denka Seiken Co. Ltd., Tokyo, Japan) using an autoanalyzer (Toshiba).
4
Metabolic Profiling of Fasting Plasma
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FPG (fasting plasma glucose, FPG) testing was performed, and fasting plasma insulin concentrations were measured via an electrochemical luminescence immunoassay. Serum total cholesterol (TC), high-density lipoprotein cholesterol (HDLC), triacylglycerols (TG), and low-density lipoprotein cholesterol (LDLC) were each measured using an autoanalyzer (Toshiba, Japan). Serum creatinine (Scr) was measured using overnight fasting venous blood samples, via Jaffe’s kinetic method. Albuminuria was measured using immunoturbidimetric tests.
5
Biochemical Data Acquisition Protocol
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Biochemical data acquiring Protocols was also described in detail in our previous publication [15 (link)]. Appointments were scheduled for blood collection. Fasting venous blood draws were performed at The People’s Hospital of Zhengzhou. All blood samples were sent to the central laboratory of The People’s Hospital of Zhengzhou. The blood samples were either disposed of within 3 h or stored at 4 °C for as long as 2 days. FPG (fasting plasma glucose, FPG) testing was performed via an electrochemical luminescence immunoassay. Serum total cholesterol (TC), HDLC, TG, and low-density lipoprotein cholesterol (LDLC) were each measured using an autoanalyzer (Toshiba, Japan). Serum creatinine (Scr) was measured using overnight fasting venous blood samples, via Jaffe’s kinetic method. Uric acid was detected by uricase-based spectrophotometry.
6
Measuring High-sensitivity C-Reactive Protein
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High-sensitivity CRP was measured by an immunoturbidimetric method (CRP II Latex X2, Denka Seiken Co. Ltd., Tokyo, Japan) using an autoanalyzer (Toshiba, Tokyo, Japan). Measurement range of this assay is 0.01 to 32 mg/dL.
7
Fasting Rat Blood Analysis
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At the end of the experimental periods, rats were fasted for at least 8 h and then anesthetized with 10% chloral hydrate. Blood samples were obtained from the right carotid artery after 4 h at room temperature and centrifuged at 3500 rpm for 15 min at room temperature. Serum was separated and uric acid and lipid levels were determined using an autoanalyzer (Toshiba, Japan).
8
Comprehensive Metabolic and Inflammatory Panel
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Serum glucose, total cholesterol, triglycerides, high density–lipoprotein cholesterol, and low density–lipoprotein cholesterol were determined by enzymatic methods using commercially available kits according to instructions. Serum CRP was measured with an autoanalyzer (Toshiba, Tokyo, Japan) and immunoturbidimetric analysis (CRP II Latex X2; Denka Seiken, Tokyo, Japan). Serum-ferritin levels were evaluated using an ELISA kit (RCD012R, BioVendor). Estimation of serum LDH was performed using an LDH-activity assay kit, (7D69-20, Abbott Diagnostics). Plasma D-dimer was measured with an ELISA kit (NB 110–8376, Novus Biologicals). Lymphocyte count were performed with an autohematology analyzer (Sysmex BC 3000).
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